Twenty years ago, Addgene co-founder Melina Fan, PhD, couldn’t get the plasmids she needed for her research, so she helped build a better way to share them. 🧬 On Aug. 1, Melina stepped into a new role as Chief Scientific Advisor. A thank-you and a look at what’s next:

blog.addgene.org

Thank You, Melina: A New Chapter After 20 Years

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Great pick for #CellLineOfTheWeek — H2B fluorescently-tagged iPS cells and plasmids from @alleninstitute.org. Proud to help get these into more labs! 🧬

allen institute@alleninstitute.org · 5d ago

Our #CellLineOfTheWeek is Histone H2B! 🧬 Our human iPS cell lines & plasmids – fluorescently-tagged to illuminate structures – are available for just the cost of shipping to accelerate scientific discovery. 📦 Distributed by @coriellinstitute.bsky.social & @addgene.bsky.social

Great to have @allostericsite.bsky.social out at #USCCN this year, meeting the folks keeping small-scale collections going strong.🧫

Eric J. Perkins, PhD (he/him)@allostericsite.bsky.social · 5d ago

Enjoying seeing some familiar faces while representing @addgene.bsky.social at the 2026 United States Culture Collection Network! #USCCN

New from @ipiproteins.bsky.social: a recombinant anti-ROBO2 antibody (IPI-ROBO2.47) targeting a key receptor in Slit-mediated axon guidance. Now available at Addgene, validated for FC and ICC in mouse and human: https://twp.ai/4ht1Pw

Missed our CRISPR pooled libraries webinar? It's on demand. 🎬 Addgene + Broad Institute break down knockout library design, how Julianna & Jacquere were optimized, and how to access them as ready-to-use lentivirus. Watch here:

www.labroots.com

CRISPR Pooled Libraries: Optimizing Knockout Library Design

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Not all adhesion molecules are created equal 🤔. NLGN1 is a key synaptic organizer, and you need an antibody that won't cross-react with other neuroligins and muddy your results. @ipiproteins.bsky.social's new recombinant anti-NLGN1 antibody (IPI-NLGN1.15) is now available: https://twp.ai/4ht1Pn

Modeling isn't just for humans — organisms have been modeling for scientists for decades! But not all models are true "model organisms." Learn more about a subset of organisms, called non-models, which can offer unexpected answers to unique questions:

blog.addgene.org

Out of the Box: The Secrets of Non-Model Organisms

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Tomorrow! Planning a CRISPR knockout screen? Addgene + Broad Institute's Genetic Perturbation Platform discuss pooled library design — and the newly optimized Julianna & Jacquere libraries for mouse & human. 📅 Jul 22 | 12 PM ET Still time to register: https://twp.ai/4htd2V

Mice! 🐁 Fruit flies! 🪰 Nematodes! 🪱 Naked mole-rats...? What do they have in common? They are models! Or, in the case of the naked mole-rat, a non-model organism. Learn more about non-models in our latest blog post:

blog.addgene.org

Out of the Box: The Secrets of Non-Model Organisms

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Did you miss our webinar? It's now on demand.🎬 Addgene and plasmidsaurus break down whole-plasmid long-read sequencing — what it catches that Sanger and short-read NGS can miss, and how to pick the right approach for your workflow. Watch it here:

www.labroots.com

How Well Do You Actually Know Your Plasmid? Long-Read Sequencing and What It Changes for Researchers

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On this day in 2012, Addgene received our first CRISPR deposit, from the Doudna lab (Doudna Lab)! pMJ806 became the first plasmid in our CRISPR collection, now a community of 1,400+ labs and 20,000+ plasmids 🧬 Find the plasmid on Addgene:

What separates a good CRISPR screen from a noisy one? Often, the guide design. Next week: Addgene + Broad Institute on the Julianna & Jacquere knockout libraries, built to balance on- and off-target effects. 📅 Jul 22 | 12 PM ET Save your spot: https://twp.ai/4htd26

Tomorrow! Don't miss our free webinar with plasmidsaurus How Well Do You Actually Know Your Plasmid? 📅 July 15 | 12 PM ET Long-read sequencing is changing construct verification — find out what you might be missing. Still time to register: https://twp.ai/4htGfw

Now available: two new plasmid kits from Mark Youles The Sainsbury Laboratory with MoClo parts for tags, promoters, terminators, and more! 🌱 Build expression constructs for your favorite plant system:

🧬🩵Plasmids for all! Thank you @kamounlab.bsky.social and colleagues. Check out all the depositing labs from @thesainsburylab.bsky.social with plasmids at Addgene: www.addgene.org/browse/organ...

Sophien Kamoun@kamounlab.bsky.social · 4w ago

Just published: Community Spirit: Plasmids for All How a decade of plasmid-wrangling at @thesainsburylab.bsky.social grew into a movement — and the unexpected friends now helping us open the doors wider. medium.com/p/community-...

Last day of #FENS2026 tomorrow! Excited for two great posters from Addgene collaborators and Ambassadors — details below

Every CRISPR screen starts with one question: which genes actually matter? A knockout pooled library helps answer it, but library design shapes what you can trust. Join us and Broad Institute for a webinar on optimizing library design. 📅 Jul 22 | 12 PM ET Register 👇

www.labroots.com

CRISPR Pooled Libraries: Optimizing Knockout Library Design

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Academia, industry, or something in between? Our latest post breaks down how to navigate the PhD job market — translating your skills, avoiding the "apply everywhere" trap, and positioning yourself for the right role. 🧬 Read the full post:

blog.addgene.org

Navigating the PhD Job Market: Choosing Between Academia, Industry, and Everything in Between

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Tomorrow at #FENS2026, don't miss Addgene Ambassador Adam Cohen presenting "Imaging Voltage Across Scales" in S34: All-Optical Voltage Imaging for Neuronal Circuit Dissection, Hall B, starting at 9:45am — along with several other talks on voltage sensors.

Plasmid errors are more common than expected, and not every method catches them all. Find the right approach in our webinar with plasmidsaurus 📅 July 15 | 12 PM ET ✅ Sanger, short + long-read ✅ Interpret quality metrics ✅ Pick the right approach Register: https://twp.ai/4htXC1

🧪 Recombinant anti-NRP1 antibodies from @ipiproteins.bsky.social are now available at Addgene. Researchers can use IPI-mNRP1.17 and IPI-mNRP1.23 for FC, IHC, and ICC in mouse and human — with no cross-reactivity to NRP2. 🎯 Check out the antibodies here:

Don't miss Addgene's own poster tomorrow at #FENS2026, July 8! PS04-08PM-680, 2:30-5pm: "Addgene Data Hub: A Platform for Sharing AAV and Antibody Characterization Data" Plus talks and posters from our Ambassadors discussing work done with amazing tools available at Addgene!

Not sure where your PhD fits outside the lab? Whether you're considering academia, industry, nonprofits, or science communication, a focused job search beats applying everywhere. Explore strategies for navigating the PhD job market on our blog:

blog.addgene.org

Navigating the PhD Job Market: Choosing Between Academia, Industry, and Everything in Between

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🎉 What a first day at #FENS2026! 8,000+ attendees and a very exciting opening ceremony featuring flamenco dancers, drummers, and hip hop dancers! 🥁💃 Tomorrow: catch Addgene Ambassadors presenting posters, and stop by our booth! Details below 🧵

Your plasmid might have a whole second career you don’t know about. 🧬 What happens when a tool developed in an academic lab finds its way into industry? We asked four Addgenies to share their perspectives. Read their insights in the full story:

www.linkedin.com

Your Research Tools Have a Wider Audience Than You Think

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🧠 Day 1 of #FENS2026 kicks off tomorrow! Excited for two great talks from Addgene Ambassadors featuring Addgene tools — details below 🧬🧵

Researchers studying axon guidance have new tools from @ipiproteins.bsky.social at Addgene: recombinant antibodies to SEMA6A and SEMA7A, validated for FC and ICC in mouse and human. The anti-SEMA7A is also validated for IHC. 🔬 Check out the antibodies here:

How well do you actually know your plasmid?🧬 Join Addgene and plasmidsaurus for a free webinar on whole-plasmid sequencing — what it catches, and why it's becoming the standard. 📅 July 15 | 12 PM ET Register here👇 https://twp.ai/4hrxVj

The Addgene Data Hub is a community platform for sharing real validation data on AAVs and antibodies — and we're featuring it at #FENS2026.

🧠⚡Electrical synapses, engineered. Kafui Dzirasa @dukemedschool.bsky.social and colleagues built connexin-based tools that enable stable, long-term modulation of neural circuits. Find the plasmids here! https://twp.ai/4hrojK