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Clàudia Morros Bernaus

@claudiamb98.bsky.social

PhD Student University of Exeter. CRISPR-Cas9 - AMR - E. coli ST131. Cornwall-Barcelona

6 Posts64 Follower96 Folgt
David Sünderhauf@davvi36.bsky.social · 4w ago

Have a look at Clàudia's first paper below! 👇🏻🥳 She realised some things weren't adding up when amplifying IS26-containing regions, and far too many PCRs later here we are.... DNA containing IS26 actually recombines in vitro, in the PCR tube! 🤯

Clàudia Morros Bernaus@claudiamb98.bsky.social · 4w ago

Very happy to share the publication of the first manuscript from my PhD! 🥳 Here, we show that PCR amplification of repetitive DNA sequences, such as AMR genes flanked by insertion sequences, can lead to the generation of artefact products that need to be interpreted cautiously. (See comments 👇)

Clàudia Morros Bernaus@claudiamb98.bsky.social · 4w ago

Very happy to share the publication of the first manuscript from my PhD! 🥳 Here, we show that PCR amplification of repetitive DNA sequences, such as AMR genes flanked by insertion sequences, can lead to the generation of artefact products that need to be interpreted cautiously. (See comments 👇)

PCR analysis of insertion sequences leads to the generation of artefact amplicons

Insertion sequences (ISs) are small, self-mobilizing DNA elements widespread across prokaryotic genomes, including chromosomes and plasmids. IS elements frequently co-localize with antimicrobial resis...

microbiologyresearch.org

Joe Weaver@joeeweaver.bsky.social · 2y ago

I will not post spoilers Emily Stevenson’s results, but I will applaud her use of animations to lead the audience through interpreting a full-screen heat map instead of just showing us a giant tapestry “as you can see…” #Microbio25