Excited to see this study, in collaboration with the De Camilli group @pdc-lab.bsky.social, published in its final format! We resolved the structure of VPS13C and found that it adopts a lipid-transfer-incompetent conformation. We also show that CaM binds VPS13s and may help regulate their function.

Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin

VPS13C, whose dysfunction causes Parkinson’s disease, adopts a lipid-transfer-inactive conformation in the cytoplasm and relies on calmodulin for proper folding and localization to membrane surfaces.

cell.com

This amazing work by @danialv.bsky.social and Bodan Hu is now out in peer-reviewed form at @cp-cell.bsky.social with a lot of new simulations! Check it out if interested in lipid transport!

Vanni Lab at UNIFR, Switzerland@labvanni.bsky.social · 8mo ago

Happy to finally share the amazing results of our long-term collaboration with Karin Reinisch’s lab on how bridge lipid-transfer proteins (BLTPs) cooperate with partner proteins to orchestrate lipid delivery. A quick thread (1/7) www.biorxiv.org/content/10.6...

Reinisch's lab work with the Vanni's lab is online in Cell!! Combining cryoEM and MD simulations, we reveal how the bridge-like lipid-transfer protein VPS13A works with the scramblase XK to deliver lipids to plasma membrane and redistribute them across the bilayer. www.cell.com/cell/fulltex...

Mechanism of lipid transfer by bridge-like protein VPS13A and the scramblase XK

The cryo-electron microscopy structure of the bridge-like lipid-transfer protein VPS13A in complex with the scramblase XK—coupled with molecular dynamics simulations—suggests that VPS13A-transported l...

cell.com

Lipid scramblases and BLTPs are key to regulate cellular lipid dynamics. Justin Korfhage and I described in-vitro methods to study lipid scrambling by protein insertases and ATG2-mediated lipid transfer on artificial lipid droplets. www.sciencedirect.com/science/chap...