Derek Severi Lundberg

@derekseveri.bsky.social

Plant / microbe biologist. PhD UNC-CH (USA), postdoc MPI for Biology (Germany), group leader SLU Uppsala (Sweden) www.LundbergLand.org

the sensitivity of "tagIMseq" will be broadly useful for CRISPR-associated transposon work. Ruling out off-target insertions in resuspended colonies with minimal effort and cost investment is critical because targeting isn't always perfect.

Derek Severi Lundberg@derekseveri.bsky.social · 4mo ago

We targeted the rpoZ 3’ UTR in a Sphingomonad. We developed a rapid transposon mapping method, “tagIMseq”, leveraging tagmentation and @plasmidsaurus.bsky.social. It even works without a DNA prep on a resuspended colony! Most colonies were good, but many were not, so screening was important.

A new paper from @dorruss12.bsky.social and Jeff Dangl on two flagellins in plant-associated Sphingomonas used for motility or host colonization. www.biorxiv.org/content/10.6...

Specialization of independently acquired flagellar FliC proteins in plant-associated Sphingomonas balances swimming and immunogenicity

Plants monitor their environment for microbial invaders using pattern-recognition receptors that detect microbe-associated molecular patterns (MAMPs). Flagellin, the main component of bacterial flagellum, contains the flg22 epitope recognized by the plant immune receptor FLS2. Immune recognition can create an evolutionary conflict, requiring bacteria to balance flagellar function and immune evasion. Here, we show that plant-associated Sphingomonads resolve this constraint by partitioning two flagellar functions, motility and colonization, across two divergent and independently acquired flagellin genes. Comparative genomics revealed widespread coexistence of FliC proteins expressing either an immunogenic variant (FliC-H) or a non-immunogenic variant (FliC-L). The non-immunogenic FliC-L is necessary and sufficient for full directional swimming, whereas FliC-H is dispensable for swimming, but sufficient for full attachment and colonization. Flagellin expression patterns mirror these functions. Thus, FLS2 recognizes the flagellar variant required for colonization rather than motility, potentially restricting colonizing bacteria from entering internal leaf and root tissues. ### Competing Interest Statement The authors have declared no competing interest. European Molecular Biology Organization, https://ror.org/04wfr2810, ALTF 743-2019 U.S. National Science Foundation, https://ror.org/021nxhr62, IOS-2416244 Howard Hughes Medical Institute, https://ror.org/006w34k90, Jefferey L. Dangl Natural Sciences and Engineering Research Council, https://ror.org/01h531d29, 532852-2019 Knut and Alice Wallenberg Foundation, https://ror.org/004hzzk67, 2021.0102

biorxiv.org

What if plant immunity doesn’t stop bacteria from arriving - but from staying? Excited to share our new Dangl Lab paper (doi.org/10.1073/pnas.2535583123), asking a fundamental co-evolutionary question: which flagellar function is targeted by plant immunity? #Coevolution #Plant-Microbiome #PNAS 🧵1/8

PNAS

Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

doi.org

When organisms look similar, you can use a tag to tell them apart. We wanted to tag our collection of diverse Sphingomonas bacteria with DNA barcodes to distinguish them. We planned to use Tn7, until we saw new reports about CRISPR-associated transposons (CASTs).

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In my experience, conference speakers are generally people who know stuff worth hearing, and not people who are great at public speaking. Listening to the content while ignoring whatever annoys you about the delivery is a good skill to cultivate, and you owe it to your intellect.

The “problem” with vaccines? They so effective at preventing deaths that they create generations of people that question whether disease was a problem in the first place because they have never experienced the horrors of a world without vaccines.

reviewing for a major international journal and 1 in 6 refs are papers written in Chinese. Titles, journal names, and abstracts are English, so you don't realize it from the ref. list.("Journal of Plant Nutrition and Fertilizers".) I can't judge these.. Journal updating policy on ref language.

1/2 What's best: a field-first or lab-first approach? No easy answers but differences between lab and field should not be seen as failure but motivate further inquiry and allow complementary discovery. Read our thoughts on this here: www.sciencedirect.com/science/arti...

Lab to field: Challenges and opportunities for plant biology

Plant-microbe research offers many choices of model and strain and whether a field-first or lab-first approach is best. However, differences between l…

sciencedirect.com