5-years in the making this paper has been a real journey! Also, a fun collaboration with @frimanscience.bsky.social @jofoth.bsky.social Edze Westra, Simon Lovell, Aras Kadioglu #mevosky #phagesky
@jofoth.bsky.social
Phage DisCo! Our targeted phage discovery method in press today: journals.asm.org/doi/10.1128/... Perfectly timed with our four summer undergrad's first successful phage hunt... disco lights in the lab to mark the occasion 🕺
New phage therapy publication!🦠 #phagesky We analysed an MRSA phage therapy case study, assessing how predictions made in vitro differed when using one (standard practice) or multiple bacteria isolates from the patient. Collaborative work: Exeter and Queen Astrid hospital doi.org/10.1093/jamb...
Predicting clinical phage therapy outcomes in vitro: results using mixed versus single isolates from an MRSA case study
AbstractAims. In phage therapy case studies, 1–3 bacteria isolates are typically tested against phages (phagogram). However, as bacteria populations differ
doi.org
New Consensus Statement in Nature Microbiology! Working with low-biomass microbiome samples? From deep subsurface rocks to human lungs, contamination is a major challenge. We offer field-to-data analysis guidelines to help keep your results clean. 📖 doi.org/10.1038/s415...
Guidelines for preventing and reporting contamination in low-biomass microbiome studies - Nature Microbiology
In this Consensus Statement, the authors outline strategies for processing, analysing and interpreting low-biomass microbiome samples, and provide recommendations to minimize contaminants.
doi.org
Whoa, F-pilus dependent phage MS2 whips off it's capsid before entry into the cell?? www.sciencedirect.com/science/arti... "Extracellular uncoating of bacteriophage MS2" #phagesky
Extracellular uncoating of bacteriophage MS2
In the early stages of infection of its host, Escherichia coli, bacteriophage MS2 sheds its icosahedral protein capsid, after which the single-strande…
sciencedirect.com
We have been working on ways to find #phage with long shelf-life and this one is a winner. 12 months freeze-thawing as much as you like, no loss of viability #phageSky #microSky rdcu.be/ewnzT
Highly stable bacteriophages PIN1 and PIN2 have hallmarks of flagellotropic phages but infect immotile bacteria
npj Viruses - Bacteriophages (phages) are viruses that kill bacteria, with potential as antibacterial agents in industrial settings, agriculture, and human health. Here, we identified two phages,...
rdcu.be
#phagesky www.biorxiv.org/content/10.1...
Temperate phages enhance host fitness via RNA-guided flagellar remodeling
Bacterial flagella drive motility and chemotaxis while also playing critical roles in host-pathogen interactions, as their oligomeric subunit, flagellin, is specifically recognized by the mammalian im...
biorxiv.org
Cell Host & Microbe@cp-cellhostmicrobe.bsky.social · last yr.Characterizing phage gene essentiality with PhageMaP: • High-throughput approach to generate genome-wide loss-of-function phage libraries. • Interrogates gene essentiality w/insights into phage genome organization, host antiviral defense & gene function www.cell.com/cell-host-mi...
#phage #phagesky www.biorxiv.org/content/10.1...
PRISM: A platform for illuminating viral dark matter
The exploration of bacteriophage diversity remains constrained by reliance on conventional plaque assays, which bias discovery toward phages that form visible plaques and propagate efficiently. The di...
biorxiv.org
💊 Antibiotics damage the gut barrier via impaired mucus production independently of the microbiota. A consideration for those using antibiotic induced depletion of the microbiome in mice https://www.science.org/doi/10.1126/sciadv.adp4119
We're advertising a fully funded PhD to explore the PK-PD of Phage therapy being developed for the treatment of Cystic Fibrosis lung infections. The post is in the Department of Pharmacology and Therapuetics at the University of Liverpool. @livuni-ismib.bsky.social @jofoth.bsky.social
Exploring the PK-PD of Phage therapy being developed for the treatment of Cystic Fibrosis lung infections at University of Liverpool on FindAPhD.com
PhD Project - Exploring the PK-PD of Phage therapy being developed for the treatment of Cystic Fibrosis lung infections at University of Liverpool, listed on FindAPhD.com
findaphd.com
Phage DisCo can be further multiplexed with three bacterial strains in co-culture, and, when tested with characterized phages, the fluorescent signal shined! Maybe you can see where the disco ball comparison comes in here. 🪩 4/
a triangle 🔺 it’s a basic shape, but no bacteria is a triangle a clothes hanger, a slice of pizza, a dorito—all triangles just not bacteria
Please sign up, it will be fun! Open for all with an interest in AMR and genomics (in the UK and elsewhere). www.targetamr.org.uk (and please give @target-amr.bsky.social a follow!)
TargetAMR – Reducing AMR through Genomics
targetamr.org.uk
TARGetAMR@target-amr.bsky.social · 2y agoWe've officially launched the #TARGetAMR network! Join us on our mission to create a #Transdisciplinary network to explore how #Genomics can combat #AMR. Together we can make a difference: Sign up now: targetamr.org.uk #AntimicrobialResistance #GlobalHealth #SaveLives
