📄 Our latest review is now out in Current Opinion in Chemical Biology! We discuss a growing class of oligonucleotide-directed proximity labeling strategies that use programmable DNA hybridization to target RNAs and genomic loci. This was a fun one to write—I hope you enjoy it! bit.ly/4h6KaRV

🔥PREPRINT ALERT🔥 We often think of mitochondria as one interconnected network. But are all mitos the same? Our new preprint-a fantastic collaboration with @yasesancak.bsky.social -reveals that organelle contacts establish specialized mitochondrial populations. Check out Yasemin’s awesome thread 👇

Yasemin Sancak@yasesancak.bsky.social · 2mo ago

Excited to share our new preprint! biorxiv.org/content/10.6... We know that mitochondria make contacts with organelles, but determining whether these contacts establish functionally distinct mitochondrial populations has been challenging.

AWESOME CONFERENCE ALERT!! I had an absolute blast at the Fusion “Genome Regulation Through RNA” meeting back in 2024, and psyched to see its upcoming return! Let’s geek out about all things Chromatin and RNA together, in Cancun! Talk abstract deadline: 10/17 Details👇

I’m a bit late to the game here, but wanted to highlight this very clever technique from @paraspeckle.bsky.social’s group! Congrats to all authors!!

Miha Modic@paraspeckle.bsky.social · last yr.

New method drop: HCR-Proxy, a modular proximity labelling approach to profile local proteome composition around RNA at nanoscale, subcompartmental resolution. Thereby we resolved nested nucleolar layers and uncovered the grammar of spatial protein partitioning. www.biorxiv.org/content/10.1...

A day late but STILL IMPORTANT

Woohoo! So psyched to see this awesome work from the Jovanovic lab out in the world! Congrats to all the authors on this tour-de-force! www.cell.com/cell/fulltex...

SPIDR enables multiplexed mapping of RNA-protein interactions and uncovers a mechanism for selective translational suppression upon cell stress

SPIDR, a massively multiplexed method that simultaneously maps dozens of RNA-binding proteins to their RNA targets at single-nucleotide resolution, uncovers new RNA-protein interactions and provides c...

cell.com

Hello! Ever wonder what's "talking to" your favorite transcript, but were too scared to ask? In our review in @cp-cellreports.bsky.social, @mardakheh.bsky.social and I highlight new RNA-focused tools for discovering RNA interactions across organizational scales. Checkit! tinyurl.com/ydn6e3ac

FUN FACT: In Europe, they celebrate #PiDay on the 3rd day of the 14th month.

TOP FIVE METABOLIC PATHWAYS …Based on whether they sound like the name of an old-school anime: 5. The Pentose-Phosphate Shunt 4. Peptidoglycan Degradation 3. Fatty Acid Beta-Oxidation 2. The Dark Reactions of the Calvin Cycle 1. Gluconeogenesis

Such awesome work from @nandangokhale.bsky.social, @ramlabuw.bsky.social, and colleagues! Fantastic seeing this paradigm-changing study out in the wild. Congrats, everyone!

Nandan Gokhale@nandangokhale.bsky.social · 2y ago

Our work on cellular RNA interactions with MAVS that promote antiviral signaling is finally live. So grateful to our indispensable collaborators as well as reviewers who helped to strengthen our work. #RNA #innateimmunity #virology @ramlabuw.bsky.social www.science.org/doi/10.1126/...

Aw, yeah! It’s en-dash time, people!

Hallo! Does anyone have a robust protocol for coupling DNA oligos to proteins? The oligo will be fairly long, and so protocols that require lower mass inputs are preferred. We have free-rein over the oligo chemistry, but the protein is from a commercial source. Feel free to PM. Thanks in advance! 🙏

<Mind legitimately blown>. *Amazing* work from @aliceyting.bsky.social and colleagues, building programmable, synthetic GPCR’s. Congrats to all authors!!

Alice Ting@aliceyting.bsky.social · 2y ago

Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from gene expression and real-time fluorescence to modulation..

Just updated my phone OS, and apparently the calendar doubles as a karyotype now?

Oh, it’s ON now. Welcome, welcome, @lippilab.bsky.social!

🤯🤯🤯

Luciano A. Masullo@lumasullo.bsky.social · 2y ago

ÅNGSTRÖM-RESOLUTION FLUORESCENCE MICROSCOPY. Can we do structural biology with an optical microscope? Yes! Check out how we resolved *single bases of DNA* and much more using fluorescence microscopy, out on 24.05.2023 in Nature! @natureportfolio.bsky.social [re-post from Twitter]

In a world where you can be anything, be Reviewer #1.

Also, today I learned that the colloquial Turkish term for puberty is, “Delikanlık,” which literally translates to, “Crazy Blood-ness.” I assert once again that Turkish is the perfect language and none of you will be able to convince me otherwise.

It’s national Teachers’ Appreciation Day in Türkiye, and to all the Turkish teachers out there, Öğretmenler Günü Çok Kutlu olsun!!!

It's my great pleasure to present the next big preprint from SheqLab! An exciting application of our O-MAP platform that I hope will transform the study of nuclear architecture. If you've ever wanted to dissect the subnuclear "neighborhood" around an individual locus, read on! (1/30)

Woohooo! Fantastic seeing this out in print. Congrats to all the authors—it was an honor to play a small part in this exciting work!

Brian Beliveau@oligopain.bsky.social · 2y ago

The pSABER paper is now online! pSABER is a new method for amplifying in situ hybridization signals This work was driven by Sahar Attar and co-led by Ram Akliesh ([at] podocytes on the other app) with help from @shechnerlab.bsky.social @dschweppe.bsky.social doi.org/10.1038/s415... 🧵 1/12

This is an outrage, I tell you! AN OUTRAGE!!

Life goal attained. 🥹

Woot! Welcome to the party, @yasesancak.bsky.social !!

Woot! Welcome to the party, @aliceyting.bsky.social!

Carolyn Bertozzi@carolynbertozzi.bskyverified.social · 2y ago

Great to see Stanford colleague and chemical biologist extraordaire @aliceyting.bsky.social here at 🦋 😀

YES! More love for the HLB.

Timothée Lionnet@successprocess.bsky.social · 3y ago

📣 New preprint! Pol II forms clusters, some of which are giant: Pol II clusters at fly histone genes could fill a yeast nucleus!👇How do these structures regulate transcription? This is what Feiyue Lu, a joint postdoc w Ruth Lehmann, looked into 1/6 biorxiv.org/content/10.1...

Characteristically beautiful work from @jmtali.bsky.social and team!! A fine new addition to the growing barnyard of RNA-targeted proximity-labeling tools.

Matthew Taliaferro@jmtali.bsky.social · 2y ago

Excited to share our latest work! In it, we develop a new method for studying RNA localization via proximity labeling: OINC-seq! In contrast to other proximity-based methods, labels deposited on RNAs are read directly by sequencing without the need for biotinylation. biorxiv.org/cgi/content/...

Meanwhile, basically my whole feed on the muskier platform:

Oh, why hello there! I was wondering where all the good folk of SciTwitter went. It’s fantastic seeing you all again.