We have new a new doctor in the lab 🍾🍾🎉Congratulations @neurorussell.bsky.social for successful viva today at Oxford @oxforddpag.bsky.social

Out today in Neurophotonics, we show that cross-talk between the imaging laser and opsin in all-optical experiments can affect response dynamics beyond what has been accounted for. Imaging conditions subthreshold for evoking spikes can accelerate opsin desensitization: doi.org/10.1117/1.NP...

Desensitization of opsin responses during all-optical interrogation depends on imaging parameters https://www.biorxiv.org/content/10.1101/2025.10.02.680058v1

🚨New pre-print!🚨 Can we truly independently read and write neuronal activity at the same time using all-optical techniques? Here we show that even two-photon imaging over large fields of view with low dwell time per cell can bias neural dynamics! Read on for some potential fixes: (1/5)

Desensitization of opsin responses during all-optical interrogation depends on imaging parameters

Significance The combination of two-photon calcium imaging and two-photon optogenetic stimulation, termed all-optical interrogation, provides spatial and temporal precision when recording and manipula...

biorxiv.org

All-optical experiments are great but @neurorussell.bsky.social found that opsin desensitization due to cross-talk between the imaging laser and the opsin causes major issues. These can be ameleorated with long inter-stimulus intervals & short imaging doses. All here: www.biorxiv.org/content/10.1...

Desensitization of opsin responses during all-optical interrogation depends on imaging parameters

Significance The combination of two-photon calcium imaging and two-photon optogenetic stimulation, termed all-optical interrogation, provides spatial and temporal precision when recording and manipula...

biorxiv.org