Jo Vandesompele
@jvdesomp.bsky.social
RNA aficionado, cancer researcher, nucleic acid quantification expert, professor at Ghent University and co-founder of pxlence and InActiv Blue
In a VIB Grand Challenge project on liver disease, we determined almost 1000 blood plasma transcriptomes and made the data available in this resource paper #exRNA #liver #fibrosis #cirrhosis #MASLD @franco17ps.bsky.social www.nature.com/articles/s41...
It is rare for a Master's thesis to end up as a peer-reviewed article. Congratulations Marina Masliakova! "Integrative benchmarking and automation of clonal reconstruction of somatic mutations in single-sample tumor genome analysis" @crighent.bsky.social link.springer.com/article/10.1...
Integrative benchmarking and automation of clonal reconstruction of somatic mutations in single-sample tumor genome analysis - BMC Bioinformatics
Background Accurate identification of truncal mutations, those present in all tumor cells, is essential for understanding tumor evolution and guiding downstream analyses in cancer genomics, including ...
link.springer.com
Primer design for 3' end of polyadenylated transcripts for targeted RNA seq, in bulk, single-cell, or spatial mode. New from OncoRNALab at @crighent.bsky.social
Bioinformatics Advances@bioinfoadv.bsky.social · 6mo ago🧪 Recently published in Bioinformatics Advances: "Peakprime: A peak-guided primer design pipeline for target enrichment in 3′-end RNA-seq" Access the article: https://doi.org/10.1093/bioadv/vbag080 Authors include: @jvdesomp.bsky.social
Blood (literally and figuratively), sweat, and tears... rejection after 3 rounds of revisions by another journal, but now out. We are proud of this new type of biomarker identification concept - applied here on cell-free RNA from cancer patients @amorlion.bsky.social www.nature.com/articles/s43...
Patient-specific alterations in blood plasma cfRNA profiles enable accurate classification of cancer patients and controls - Communications Medicine
Morlion et al. analyze cell-free RNA in blood plasma from people with and without cancer to identify cancer-specific patterns. Individual patients exhibit unique changes in cell-free RNA, and the numb...
nature.com
The spatial team at OncoRNALab @ds-ugent.bsky.social posted a benchmarking preprint on DNA barcode decoding strategies www.researchsquare.com/article/rs-8... @brechtsoulliaert.bsky.social
Benchmarking DNA barcode decoding strategies under high error rates
Background: DNA barcoding enables multiplexed identification of biomolecules in pooled sequencing experiments, with broad applications including spatial transcriptomics. Photolithographic synthesis of...
researchsquare.com
Still possible to follow online. #EUDIP2025 All about digital PCR #dPCR digpcr.ugent.be/EUDIP2025/ great talks by Allen Chan, Noemi Castellucci, Wim Trypsteen.
The model of harmonizing general and domain-specific guidelines provides a scalable blueprint for improving reproducibility across complex biomarker development workflows in molecular diagnostics. The case for EV and qPCR www.oaepublish.com/articles/evc...
MISEV and MIQE: integrating domain-specific and general standards to strengthen extracellular vesicle biomarker research
Extracellular vesicles (EVs) have significant potential as therapeutic agents and as sources of diagnostic, predictive, and prognostic nucleic acid biomarkers. However, variability in EV workflows and...
oaepublish.com
Are you also a desperate cell-free RNA researcher? It’s not always a grim story. Philippe Decruyenaere is defending his excellent PhD thesis on extracellular RNA in non-Hodgkin lymphoma. @crighent.bsky.social
"Eindelijk beeld bij het verhaal van onze genen" Dit artikeltje wou ik toch even delen. Wetenschappelijk onderzoek helder uitleggen is niet makkelijk, en toch is het belangrijk. Hier legt Hanne, een doctoraal onderzoeker in ons OncoRNALab (@crighent.bsky.social @ds-ugent.bsky.social) ...
7 years in the making... 1.6 liters of blood and 11 billion sequencing reads to compare 456 extracellular transcriptomes. Our best practices for blood plasma extracellular RNA profiling have been published as a peer-reviewed article. communities.springernature.com/posts/the-ro...
The road to discovery - a collaborative effort towards new insights in blood plasma RNA profiling
After more than seven years of research, our extracellular RNA quality control (exRNAQC) study has culminated in a scientific publication. This milestone is not just about sharing new findings – it re...
communities.springernature.com
After 16 years, an update of the qPCR #MIQE guidelines is out on how to design, execute, analyze, and report on #qPCR experiments doi.org/10.1093/clin.... The original paper from 2009 accumulated 17,000 citations (scholar.google.be/citations?vi...). @genequan.bsky.social @jimhuggett.bsky.social
MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines
AbstractBackground. In 2009, the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines established standards for
doi.org
👉 Finally the update of the MIQE Guidelines is out in Clinical Chemistry 👍 𝗠𝗜𝗤𝗘 𝟮.𝟬: 𝗥𝗲𝘃𝗶𝘀𝗶𝗼𝗻 𝗼𝗳 𝘁𝗵𝗲 𝗠𝗶𝗻𝗶𝗺𝘂𝗺 𝗜𝗻𝗳𝗼𝗿𝗺𝗮𝘁𝗶𝗼𝗻 𝗳𝗼𝗿 𝗣𝘂𝗯𝗹𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗤𝘂𝗮𝗻𝘁𝗶𝘁𝗮𝘁𝗶𝘃𝗲 𝗥𝗲𝗮𝗹-𝗧𝗶𝗺𝗲 𝗣𝗖𝗥 𝗘𝘅𝗽𝗲𝗿𝗶𝗺𝗲𝗻𝘁𝘀 𝗚𝘂𝗶𝗱𝗲𝗹𝗶𝗻𝗲𝘀 lnkd.in/dhWhtZqC
New evidence @nejm.org today that age-related clonal hematopoiesis (CHIP) has a driver role in evolution of solid cancers www.nejm.org/doi/full/10.... www.nejm.org/doi/full/10....
Clonal Hematopoiesis as a Driver of Solid Tumors | NEJM
Clonal hematopoiesis is the expansion of a genetically related population of hematopoietic stem and progenitor cells that disproportionately contribute to blood-cell production.1 Clonal hematopoies...
nejm.org
Ik heb zopas de resulaten doorgestuurd van de waterkwaliteit in de Tichelbeek t.h.v. de Brandstraat in Zulte, als deel van het grootste burgeronderzoek ooit. Ben je ook benieuwd naar de resultaten? Neem dan een kijkje op www.watermonsters.be. Vanaf 7 juni zijn alle resultaten gekend. #watermonsters
Proud OncoRNALab contribution to #CRIG LECR lab’s latest protocol paper on spheroids www.nature.com/articles/s41...
Sequential orthogonal assays for longitudinal and endpoint characterization of three-dimensional spheroids - Nature Protocols
The authors present a protocol for the self-assembly of single-cell suspensions into spheroids by the liquid overlay method, followed by a modular framework of orthogonal assays to phenotype spheroids...
nature.com
The case for curiosity-driven research www.statnews.com/2025/04/03/b... h/t former OncoRNALab PhD fellow @verwilt.bsky.social
Why we study shrimp on treadmills: The case for curiosity-driven research
“If we cut or limit funding for curiosity-driven research, we risk shutting down the pipeline of future innovation,” writes Carole LaBonne of Northwestern.
statnews.com
Ever wondered what RNA in your body fluids can reveal about your health? 🤔 Eva Hulstaert took on the challenge, collecting and analyzing various biofluids to uncover hidden insights. 👩🔬 Anneleen Decock & I share the story of this fascinating quest in EOS Wetenschap (in Dutch): tinyurl.com/4ev7vk83
Wat onze lichaamssappen ons vertellen
Rond 400 voor Christus ontwikkelde Hippocrates, de grondlegger van de moderne geneeskunde, zijn leer der humores. Hij stelde dat de gezondheid van de mens af...
tinyurl.com
Did you know that our body contains dozens of different biofluids (like blood, sweat, urine, saliva, ...) and that these give insights into our health? OncoRNALab members @amorlion.bsky.social and Anneleen Decock wrote a fascinating story (in Dutch) in the science magazine Eos - lnkd.in/ern_2kDW
Transitioning from SYBR Green I qPCR assays to cost-efficient multiplex digital PCR is a breeze with universal Rainbow detection probes.
#OncoRNALab current and past members list is growing on @bsky.app bsky.app/profile/jvde...
Version 2 of our tail biomarker gene preprint is available. Novel way of exploiting cell-free RNA in blood plasma from cancer patients. doi.org/10.1101/2023... #liquidbiopsy
Open vacancy for PhD fellowship at Ghent University to work on novel spatial -omics platform www.ugent.be/en/work/scie...
PhD Student
ugent.be
