Tessera
Folgen

The Drew Weissman Lab

@drewweissmanlab.bsky.social

Bringing mRNA therapeutics to the world. University of Pennsylvania https://www.med.upenn.edu/weissmanlab/

32 Posts227 Follower77 Folgt
The Drew Weissman Lab@drewweissmanlab.bsky.social · 10mo ago

📣Today @ 4pm: RNA Collaborative - NCI RNA Biology Initiative & Penn Institute for RNA Innovation, featuring BMB graduate student Rachel Braun “Selective decay of interferon mRNAs controls antiviral immunity and innate memory" us02web.zoom.us/webinar/regi...

Welcome! You are invited to join a webinar: RNA Collaborative - NCI RNA Biology Initiative & Penn Institute for RNA Innovation. After registering, you will receive a confirmation email about joining t...

Featuring: “Mechanism of mRNA decay inhibition by a bacterial pathogen effector" Dr. Eugene Valkov Stadtman Investigator, RNA Biology Laboratory, Center for Cancer Research, National Cancer Institut...

us02web.zoom.us

The Drew Weissman Lab@drewweissmanlab.bsky.social · 12mo ago

🎉Our newest pre-print is now online on bioRxiv! Anionic lipids modulate mRNA-lipid nanoparticle immunogenicity and confer protection in a mouse model of multiple sclerosis @jilianmelamed.bsky.social www.biorxiv.org/content/10.1...

Anionic lipids modulate mRNA-lipid nanoparticle immunogenicity and confer protection in a mouse model of multiple sclerosis

The modularity of mRNA-lipid nanoparticle (mRNA-LNP) platforms has enabled their rapid adaptation from infectious disease vaccines to emerging applications in immune-mediated disorders. However, exten...

biorxiv.org

The Drew Weissman Lab@drewweissmanlab.bsky.social · last yr.

Harnessing the Electron-Withdrawing Inductive Effect of One-Component Ionizable Amphiphilic Janus Dendrimers Unveils Cation−π Interactions and Their Important Roles to Targeted mRNA Delivery | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...

Harnessing the Electron-Withdrawing Inductive Effect of One-Component Ionizable Amphiphilic Janus Dendrimers Unveils Cation−π Interactions and Their Important Roles to Targeted mRNA Delivery

It is expected that current medicine will be complemented or even replaced with genetic nanomedicine, which relies on the targeted delivery of nucleic acids. Delivery of mRNA is accomplished using viral and synthetic four-component vectors which deliver the nucleic acid predominantly to the liver. Our laboratories elaborated a one-component ionizable amphiphilic Janus dendrimer (IAJD) which targets the delivery of mRNA and, therefore, is complementary to viral and synthetic vectors. Here we report the study of a library of IAJDs containing derivatives of the natural gallic acid (GA) as branched hydrophobic domain and two ionizable amines connected via four linker length. The linker length mediates an electron-withdrawing inductive effect, which changes the pKa of the IAJDs. The same pKa trend was observed in a previously reported pentaerythritol (PE)-library, which has structures identical to those of the GA-library except that it does not contain the aromatic ring. Unpredictable, the GA-library of IAJDs has consistently higher pKa values than the PE-library, although the linker length-pKa dependence follows the same trend in both libraries. This trend indicates an unexpected cation−π interaction which explains some advantages of GA-IAJDs, including the 109 p/s total luciferase activity reported here.

pubs.acs.org

@KingOfPathogens@kingofpathogens.bsky.social · last yr.

We're not the first to use IL-12 as an adjuvant - but having worked on IL-12 for >30 years this was still a surprise at how much this study taught us about mRNA vaccines and their utility. Here are two others below: www.science.org/doi/10.1126/... @cytokinesociety.bsky.social

An Il12 mRNA-LNP adjuvant enhances mRNA vaccine–induced CD8 T cell responses

IL-12 is dispensable for CD8 T cell responses to mRNA vaccination, but an Il12 mRNA-LNP enhances CD8 T cell memory and protection.

science.org

Dr. Donita C. Brady of Brady Lab UPenn@bradylabupenn.bsky.social · 2y ago

Several months ago, I was honored to be invited by JMB Editor-in-Chief Mike Summers to write a Perspective for the Pioneers in Molecular Biology special issue. It was a chance to reflect on the journey that led me here. You can read the pieces here: tinyurl.com/2pb967u9 & tinyurl.com/kkwfynt2

Making Every Penny Count: Kinase Signaling Transduction, Copper Homeostasis, & Nutrient Sensing

I am the Harrison McCrea Dickson, MD, and Clifford C. Baker, MD Presidential Associate Professor of Cancer Biology at the University of Pennsylvania P…

tinyurl.com