Our photoswitchable HaloTag is out in Angewandte Chemie @angewandtechemie.bsky.social ! We now also show that this system can be used to control emitter density in SMLM. Congrats to Franzi, Bego, and our amazing collaborators. Check it out: onlinelibrary.wiley.com/doi/full/10....

A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells

Photoswitchable fluorophores are critical for advanced bioimaging. Here, we develop a photoswitchable self-labeling HaloTag that can reversibly modulate the emission of a bound fluorogenic dye via a ....

onlinelibrary.wiley.com

This work is now out @pnas.org. Result of fantastic collaborations with @so-lets-kilab70.bsky.social and Jason vevea @St Jude. Look out for two cool fluorescent ligands.

Optimizing multifunctional fluorescent ligands for intracellular labeling | PNAS

Enzyme-based self-labeling tags enable the covalent attachment of synthetic molecules to proteins inside living cells. A frontier of this field is ...

pnas.org

Pratik Kumar@dyechemist.bsky.social · 2y ago

🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.

#AwardHighlight Mousumi Baruah, post-doctoral researcher from Dr Pratik Kumar's lab, is the recipient of the National Post-doctoral Fellowship (N-PDF) by The Anusandhan National Research Foundation, India Congratulations!🎉 @dyechemist.bsky.social

#CampusVisit We recently welcomed 11th & 12th-grade students from DPS (N). They interacted with Dr Pratik Kumar & visited the labs of Prof Sanjay Sane & Dr Shashi Thutupalli. The students learned how science is interdisciplinary and not done in silos. @dyechemist.bsky.social @stpalli.bsky.social

#CampusVisit Students of Bangalore International School visited NCBS last week. They started the visit with an activity to learn critical thinking. @dyechemist.bsky.social spoke about life as a scientist & Aksha Chowdhary, wildlife master’s alum, shared her journey. They also visited the Ear Lab

Come and do some chemistry for biology with us!

National Centre for Biological Sciences, Bengaluru@ncbsbangalore.bsky.social · last yr.

#NewFaculty Welcome to the campus Dr Pratik! Dr Pratik is a 'tool builder' working at the intersection of Biology and chemistry. We look forward to the contributions from his lab. Know more: news.ncbs.res.in/spotlight/nc... @dyechemist.bsky.social 🖊️@rupsykhurana.bsky.social

If making chemical tools for biology excites you, consider applying to the NCBS graduate program! My lab plans to hire 1–2 graduate students this cycle. More info on my lab @ dyecraftlab.com

National Centre for Biological Sciences, Bengaluru@ncbsbangalore.bsky.social · last yr.

#AdmissionsAlert! The National Centre for Biological Sciences announces the opening of online applications for admission to its PhD programme through National Exam Route. Application Deadline: 24th March 2025 Joining: 1st August 2025 Apply here: bit.ly/4igBGFK

Alternate abstract image for our latest manuscript

Luke Lavis@rhodamine110.bsky.social · 2y ago

Our multifunctional ligands to BRD4 and histone H3.3:

Ow boy! Just when I thought I couldn't be impressed by a JQ1 containing compound anymore... it gets mashed with my favourite Janelia Fluor dye (JF635) and a halotag ligand. Exceptional work #chembio

Pratik Kumar@dyechemist.bsky.social · 2y ago

🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.

🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.

New to #ubiquitin? Been with it for a while? Either way this review may be for you 🤩 Excited to share our review article in @molcell.bsky.social, diving deep into everything #ubiquitin Read here 👉: kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F... @cellpress.bsky.social @wehi-research.bsky.social

We made a photoswitchable HaloTag (psHaloTag), which can reversibly turn-on fluorogenic dyes upon illumination 💡. Congrats to Franzi, Bego and all co-authors, check out our preprint below 👇 www.biorxiv.org/content/10.1...

Super excited to start my lab @NCBS_Bangalore in March 2025! The lab will primarily be powered by chemistry, and we will build chemical tools for biology with a strong focus on fluorescence imaging. We will collaborate widely and are open to any relevant areas of biology.