Another #nasal feature not discussed here is our finding that >70% of #COVID19 outpatients produced transient, virus-induced anti-IFNα autoantibodies in the nose, associated with BETTER outcomes www.science.org/doi/10.1126/.... Host's antiviral immunity rather than a breach of immune tolerance?

Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19

Nasal IgA1 autoantibodies against IFN-α are associated with better COVID-19 prognosis, including fewer symptoms and robust anti–SARS-CoV-2 immunity.

science.org

Eric Topol@erictopol.bsky.social · 4w ago

To get better protection against respiratory infections and transmission we need to achieve nasal immunity. A new @cp-immunity.bsky.social review www.cell.com/immunity/abs...

Another #nasal feature not discussed here is our finding that >70% of #COVID19 outpatients produced transient, virus-induced anti-IFNα autoantibodies in the nose, associated with BETTER outcomes www.science.org/doi/10.1126/.... Host's antiviral immunity rather than a breach of immune tolerance?

Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19

Nasal IgA1 autoantibodies against IFN-α are associated with better COVID-19 prognosis, including fewer symptoms and robust anti–SARS-CoV-2 immunity.

science.org

Eric Topol@erictopol.bsky.social · 4w ago

To get better protection against respiratory infections and transmission we need to achieve nasal immunity. A new @cp-immunity.bsky.social review www.cell.com/immunity/abs...

This was one of the most complex projects I have ever been involved in. Took us the better part of a decade, but I thoroughly enjoyed every minute of it. Especially the intensive and extensive interactions with @wsdewitt.github.io, @matsen.bsky.social, and @tylernstarr.bsky.social

William DeWitt@wsdewitt.github.io · 2mo ago

This started in 2019 as daydreamy PhD student musings with Tatsuya Araki. We're as excited as ever about germinal centers as a platform for experimental evolution. Many thanks to PIs @victora.bsky.social @matsen.bsky.social, co-1st authors Ashni Vora and Tatsuya, and many other key collaborators!

New preprint‼️ How to make human #Bcells as protein factories for #CellTherapy? We show that human pluripotent stem cells (hPSCs) can generate functional B cells and #PlasmaCells! Great collab with Gordon Keller (UHN) and Deepta Bhattacharya (U Arizona) More soon! #BYourCell doi.org/10.64898/202...

'(...) these findings demonstrate the efficient generation of B cells from hPSCs and, in doing so, provide a system for further investigating the earliest stages of human B lymphopoiesis and a source of appropriately staged plasma cells for future therapeutic applications.' #Immunology #Preprint

New preprint‼️ How to make human #Bcells as protein factories for #CellTherapy? We show that human pluripotent stem cells (hPSCs) can generate functional B cells and #PlasmaCells! Great collab with Gordon Keller (UHN) and Deepta Bhattacharya (U Arizona) More soon! #BYourCell doi.org/10.64898/202...

“first drafts of Results, Discussion, and Introduction should be the trainee’s own work. AI can help with grammar…tightening prose…critiquing the logic of an argument you’ve already made. But the hard part of forming the argument needs to be yours.’ blekhman.substack.com/p/you-need-t...

You need to make AI guidelines for your lab

Here's why you should, and how to start

blekhman.substack.com

Congratulations to Shaila Sachdev for defending her undergraduate Honors Thesis on human early-life B cells and receiving the Highest Honors! @emoryuniversity.bsky.social Shaila made exciting discoveries about the signaling required to activate or inhibit human #early-life #Bcells. #BYourCell

Save the date! www.keystonesymposia.org/conferences/...

B Cell and Antibody Responses | Keystone Symposia

Join us at the Keystone Symposia on B Cell and Antibody Responses, February 2027, in London, with field leaders!

keystonesymposia.org

To strengthen the European research on early life immunology we have started the EFIS study ELINE Info and seminar announcement 👇🏼 #EFIS#ELINE#immunology

Early-life Immune Network Europe@efis-eline.bsky.social · 6mo ago

We are excited to kick-off of our seminar series on March 5, 4pm CET 🥳 🎤 Petter Brodin – A seeding reaction to colonizing microbes in human newborns 🎤 Eva Stange – Neonatal liver niches program T cell tolerance Register here: www.efis-eline.eu/seminars #EarlyLifeImmunity #Immunology #EFIS

Too much data, too little thinking. A important essay from Ruslan Medzhitov on the importance of understanding data, not just generating it. A must read. @Yale www.nature.com/articles/s41...

On the balance of knowledge - Nature Reviews Immunology

Ruslan Medzhitov shares his thoughts on the balance between generating data and developing theories in immunology, with a focus on exploring the rules that govern complex systems.

nature.com

The Yewdell Lab at Genentech is seeking a summer intern for 2026!! An excellent opportunity to learn how we combine fundamental, basic research with drug discovery to create novel therapeutics, and to see what biotech is all about. Apply now! roche.wd3.myworkdayjobs.com/ROG-A2O-GENE...

Download our large database of postdoc fellowships in all fields of research. Database freely available to all; 281 fellowships. Download here: research.jhu.edu/rdt/funding-...

Researchers in #ScienceTranslationalMedicine find a distinct #Bcell and antibody profile in infants and mothers in an Older Order Mennonite farming community, associated with lower incidence of egg #allergy. https://scim.ag/48rPBGS

Farm exposure in infancy is associated with elevated systemic IgG4, mucosal IgA responses, and lower incidence of food allergy

Infants from a traditional farming community exhibited antibody profiles that were found to be associated with lower incidence of egg allergy.

scim.ag

This #Preprint reports that B6 (but not wild mice) have a truncated PDL2, missing an active intracellular signalling domain: "Surprisingly, in C57BL/6J (B6) mice PD-L2 has only a 5-amino acid intracellular domain, and the same is true for several other laboratory mouse strains" #Immunology

PD-L2 Regulates Natural Antibody and IL-10 Secretion by B-1 cells

B-1 cells are innate-like lymphocytes that play a critical role in homeostasis by secreting natural antibodies, typically IgM, and immunosuppressive molecules such as IL-10. However, the regulation of...

biorxiv.org

$15k stipend to support 9 weeks of full time summer research! For rising juniors/seniors who are able to travel to and obtain housing at the location of the lab they are placed in! I did an HHMI summer experience in 2013 and it changed everything for me! Apply…or tell someone to apply! 🧪🧠

Leslie B Vosshall PhD@leslievosshall.bsky.social · 9mo ago

@hhmi.org Summer Undergraduate Research Experience - #CechFellows named in honor of Prof Tom Cech Deadline to apply: 12/22/2025 Spend 9 weeks in a paid, mentored biomedical research experience in an HHMI lab. See you next summer!!! www.hhmi.org/programs/cec...

FlowLITE: A protocol to characterize and quantify total antibody isotypes in human plasma using flow cytometry #protocol #starprotocols #cellpress

FlowLITE: A protocol to characterize and quantify total antibody isotypes in human plasma using flow cytometry

FlowLITE is a flow cytometry bead-based assay that enables multiplexing and high-throughput quantification of all antibody isotypes from humans, mice, or other species, using small volumes of any fluid specimen. Here, we detail a FlowLITE protocol to quantify and characterize antibody light-chain and isotype expression in human plasma. We describe steps for titrating the capture and reveal antibodies, multiplexing assay beads, and staining samples. We then describe proced...

dlvr.it

#FlowLITE is a modular, easy-to-build, bead-based assay for characterizing & quantifying absolute number of antibodies in small volumes (~10μL) of any fluid specimen. This is Justin Hahm’s Honors Thesis project (Highest Honors!). He was mentored by PhD student Alex Nazzari, the co-first author. 2/4

While #FlowLITE quantifies total antibodies, #FlowBEAT also quantifies antibody specificities to defined antigens. Both protocols can be adapted to your research needs. 4/4 star-protocols.cell.com/protocols/4572

Cell Press: STAR Protocols

STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

star-protocols.cell.com

For context: FlowLITE was based on our previous technology, FlowBEAT, which we developed to measure human antibody isotypes against #SARSCoV2 and autoantigens in the #nasal #mucosa of #COVID19 patients (Sci Transl Med, 2024) 3/4 www.science.org/doi/10.1126/...

Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19

Nasal IgA1 autoantibodies against IFN-α are associated with better COVID-19 prognosis, including fewer symptoms and robust anti–SARS-CoV-2 immunity.

science.org

No more #ELISA assays?

Eliver Ghosn@eliverghosn.bsky.social · 9mo ago

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572 Is it time to retire the ELISAs for #antibody measurements? 1/4

Our 🌈 Annual Cook-off event keeps growing! We build community around great food, drinks, and music. Thank you for the delicious dishes and fantastic company. The 2025 Chef Laureate goes to...

You will identify immune mechanisms underlying neuroinflammatory autoimmune diseases in children vs adults--autoimmune encephalitis (AE) and multiple sclerosis (MS). You will apply multi-omics single-cell assays to study the role of B/plasma cells and antibodies using patient samples (CSF and blood)

We are #hiring a postdoctoral fellow! Please share! Join our B-cell team @Emory to lead NIH-funded projects on human neuro-immunology and autoimmunity in a co-mentorship and collaboration with neuroscientist Dr. Grace Gombolay. Please apply here: faculty-emory.icims.com/jobs/156609/... #BYourCell

We are #hiring a postdoctoral fellow! Please share! Join our B-cell team @Emory to lead NIH-funded projects on human neuro-immunology and autoimmunity in a co-mentorship and collaboration with neuroscientist Dr. Grace Gombolay. Please apply here: faculty-emory.icims.com/jobs/156609/... #BYourCell

Main dish or Appetizer 1. Adas Polo & Lamb (Fatemeh Yousefian and Masoud Ilkhani) 2. Lasagna Bolognese (Alex Nazzari & Matt Stern) Dessert 1. Mohanthal (Hardy Nariya) 2. Cesta di Frutta @katyely.bsky.social

No more #ELISA assays?

Eliver Ghosn@eliverghosn.bsky.social · 9mo ago

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572 Is it time to retire the ELISAs for #antibody measurements? 1/4

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572 Is it time to retire the ELISAs for #antibody measurements? 1/4

Our new FlowLITE protocol for simultaneously quantifying all (human and mouse) #antibody isotypes in a single tube using #flowcytometry is out in STAR Protocols: star-protocols.cell.com/protocols/4572 Is it time to retire the ELISAs for #antibody measurements? 1/4

Our 🌈 Annual Cook-off event keeps growing! We build community around great food, drinks, and music. Thank you for the delicious dishes and fantastic company. The 2025 Chef Laureate goes to...