Our work on NPC biogenesis is now out! Wanlu Zhang investigated the role of FG-Nups in NPC reassembly, pore dilation, and the nuclear envelope remodelling using acute depletions, correlative EM, and MINFLUX imaging 💫. Thanks, Wanlu and all the collaborators!

Wanlu Zhang@wanluzhang.bsky.social · 11mo ago

Excited to share the preprint from my postdoc work @ellenberglab.bsky.social! 🌟 During mitotic exit, cells rebuild thousands of nuclear pores within minutes. How do tiny holes expand to selective channels, and how is this coordinated with the nuclear envelope remodeling? 🔗 doi.org/10.1101/2025...

🔬🧬 New in @natcomms.nature.com‬: Nanoscale 3D DNA tracing in non‑denatured cells maps the #3Dgenome at nm‑resolution and reveals Cohesin‑dependent dynamic loops! 🚀 #OpenAccess  www.nature.com/articles/s41... @ellenberglab.bsky.social @jungmannlab.bsky.social #Genomics #SuperResolution

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications

A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

nature.com

Our new non-denaturing nanoscale 3D DNA tracing technology provides single-cell (and sister-chromatid specific!) readout of chromatin structure with 10kb genomic resolution - Check it out! www.nature.com/articles/s41... Congratulations to Kai and Oyvind!!

Nanoscale 3D DNA tracing in non-denatured cells resolves the Cohesin-dependent loop architecture of the genome in situ - Nature Communications

A nanoscale 3D DNA tracing workflow visualizes Cohesin-dependent loops in single, structurally well-preserved cells. Computer simulations based on the tracing data give further insight into how Cohesi...

nature.com

This seems like a really important paper comparing chromosome tracing methods, demonstrating that classical denaturing DNA-FISH and chromosome tracing leads to substantial distortion of 3D genome structure, likely due to the high-temperatures involved (70-90C): www.nature.com/articles/s41...

Check out Andi's #Bluetorial for a summary of our findings 🙌

Andi Brunner@andibrunner.bsky.social · last yr.

Mitotic chromosomes form through long, overlapping and self-repelling DNA loops, generated by the Condensin complexes! Check out our newest paper here, and see below for a short Bluetorial! www.cell.com/cell/fulltex...

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪 New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation. @ellenberglab.bsky.social @andibrunner.bsky.social www.embl.org/news/science...

How chromosomes shape up for cell division | EMBL

EMBL scientists have shown how overlapping loops of DNA stack upon each other in dividing cells to give rise to rod-shaped chromosomes

embl.org

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social! Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩 www.sciencedirect.com/science/arti...

Nanoscale DNA tracing reveals the self-organization mechanism of mitotic chromosomes

How genomic DNA is folded during cell division to form the characteristic rod-shaped mitotic chromosomes essential for faithful genome inheritance is …

sciencedirect.com

EMBL@embl.org · last yr.

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪 New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation. @ellenberglab.bsky.social @andibrunner.bsky.social www.embl.org/news/science...

Our new findings on how chromosomes get ready for cell division are now published in @cellpress.bsky.social! Congratulations, Kai, @andibrunner.bsky.social and everyone else involved! 🤩 www.sciencedirect.com/science/arti...

Nanoscale DNA tracing reveals the self-organization mechanism of mitotic chromosomes

How genomic DNA is folded during cell division to form the characteristic rod-shaped mitotic chromosomes essential for faithful genome inheritance is …

sciencedirect.com

EMBL@embl.org · last yr.

Did you know that DNA must form overlapping loops to give rise to the well-known X-shape of chromosomes? 🧬🧪 New research from EMBL's Ellenberg Group provides new insights into the process of chromosome formation. @ellenberglab.bsky.social @andibrunner.bsky.social www.embl.org/news/science...

We are #hiring! We have two open postdoc/research scientist positions to join our #ERC funded project MITOFOLD to resolve the organization of mitotic chromosomes! 1) experimental biologist shorturl.at/LjGEM 2) data scientist shorturl.at/nDdhQ Please share! 🙌 We look forward to your application! 🤩

Congratulations to @andibrunner.bsky.social and the team, and many thanks to the lab of Jan-Michael Peters for support! We found that the interphase and mitotic chromatin loop organization have more in common than previously thought: In both stages big loops are built first, small ones second

Journal of Cell Biology@jcb.org · 2y ago

@andibrunner.bsky.social et al. @ellenberglab.bsky.social show that interphase organization into Cohesin-driven loops occurs in a sequential and hierarchical manner - just like the mitotic organization by Condensins. https://buff.ly/426gIEu @wanluzhang.bsky.social

My first own project is published: Despite major differences in chromosome shape during #interphase and #mitosis, the underlying #loop-extruder based organization is highly similar A new data resource and new insights into human chromatin biology! 🧬🥳

Journal of Cell Biology@jcb.org · 2y ago

@andibrunner.bsky.social et al. @ellenberglab.bsky.social show that interphase organization into Cohesin-driven loops occurs in a sequential and hierarchical manner - just like the mitotic organization by Condensins. https://buff.ly/426gIEu @wanluzhang.bsky.social

Our excellent postdoc Wanlu Zhang is at #CellBio24 and will talk about her unpublished NPC biogensis work tomorrow! Check it out and say hello 🙌🏼

We are now on Bluesky! Follow us for exciting #Science around #CellDivision and nuclear organization, illuminated using advanced #imaging #technology - like our last preprint on mitotic chromosome structure and organization: tinyurl.com/yaznnkku