Published an op-ed for @cnn.com: “Nobel laureate: I owe America my success. Today, its scientific future is in danger.” A personal reflection on what’s at stake as science funding gets slashed. I’d be grateful if you could amplify both in and beyond the science world. www.cnn.com/2025/04/09/h...

Nobel laureate: I owe America my success. Today, its scientific future is in danger | CNN

Dr. Ardem Patapoutian says he watches “with deep sadness as the United States’ remarkable scientific enterprise, which took generations of hard work and national investment to build, faces a concerted...

cnn.com

I could not be more excited to have our lab's first story online where we report our discovery that HDACs ~reverse~ their activity to ADD acyl groups to lysine! We found this for our favorite ketone body, BHB, but this pathway controls other lysine modifications too!🧵 www.nature.com/articles/s41...

Reversible histone deacetylase activity catalyzes lysine acylation - Nature Chemical Biology

Tsusaka et al. discover that histone deacetylases, which are well known to remove protein modifications, such as lysine acetylation and β-hydroxybutyrylation, can also reverse their chemical activity ...

nature.com

Awesome turnout @standupforscience.bsky.social SF today! The Oregon Trail inspired sign was one of my favorite!

Welcome to the Bluesky account for Stand Up for Science 2025! Keep an eye on this space for updates, event information, and ways to get involved. We can't wait to see everyone #standupforscience2025 on March 7th, both in DC and locations nationwide! #scienceforall #sciencenotsilence

Just look at these paragraphs from the AP story. Hegseth called Brown unqualified solely because he's Black. Then they fired him... and replaced him with a white guy so indisputably unqualified that he requires a Presidential waiver. This is what "merit" means to them. apnews.com/article/trum...

Can I give some unsolicited advice? When communicating on this catastrophe, don’t lead with “I know the NIH is inefficient and needs serious reform, but…”. You are undercutting the entire message and buying into the frame of the arsonists. This is not a scientist audience. No “to be fair” points

I’m reaching out to my vendors to push them to use their lobbying infrastructure to point out the devastating effects the NIH change will have on the USA’s R&D engine.

Carl T. Bergstrom@carlbergstrom.com · 2y ago

1. Today the NIH director issued a new directive slashing overhead rates to 15%. I want to provide some context on what that means and why it matters. grants.nih.gov/grants/guide...

all y'all academics who have been naively convinced IDC only goes to useless sub deans are about to find out. they are going to claw the real costs of research out of your unchanged direct costs.

Indirect rates are not determined by NIH. Instead, they are the product of elaborate negotiations between each institution and the “cognizant federal agency” according to an Office of Management and Budget document, Circular A-21. 1/n

1. Today the NIH director issued a new directive slashing overhead rates to 15%. I want to provide some context on what that means and why it matters. grants.nih.gov/grants/guide...

We just upgraded one of our Illumina NovaSeqX’s to enable the new 25B 100 and 200 cycle kits. New software is supposed to slightly raise PF% and we are seeing that. 10X GEX libraries previously generated 32B reads per flow cell. We seeing over 33B, almost 67B reads in 24 hours for a dual FC run!

We just got our n6 Tec iconPCR installed. The system monitors NGS library amp with real time functions but each of the 96 wells is independently temperature-controlled and monitored. This is super helpful when you have varying inputs. www.n6tec.com

The new MiSeq i100 should be good to check pooling for the NovaSeqX. Catherine's group is seeing good consistency between the two platforms, but from first glance, it does appear the MiSeq i100 is more consistent with the older iSeq (blue and orange lines).

Catharine.Aquino@catharineaquino.bsky.social · 2y ago

One application for which we want to use the Illumina Miseq i100 is to QC pools pre-NovaseqX run. Our initial tinkering, involving sequencing the same pool on the i100, Iseq, & X, show that the read counts on the i100 are closer to the Iseq counts, and both are reasonably close to the X counts.

The #illumina #NovaSeqX 25B 100 cycle kits should be released sometime this month. These further reduce the cost of applications like single-cell sequencing by ~20%. Besides a cost reduction, the runs will take ~24 vs 48 hours, increasing the throughput of our machines.

Hello World! I direct the UCSF Center for Advanced Technology (CAT), an on campus genomics center that does a lot of development work in the NGS space. We offer Illumina and PacBio sequencing to groups on and off campus. Other interests in molecular biology and lab automation. cat.ucsf.edu

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cat.ucsf.edu

Remember when @pacbio.bsky.social introduced Kinnex and customers suddenly got >40M HiFi transcripts per run? I was tasked to solve scaling up Iso-Seq reference-free clustering and had less than a month. There were a lot of #bioinformatics challenges to be solved More info on isoseq.how or read on🧵

After many dozens of children died preventable deaths of measles in Samoa, a vaccination campaign was successful. Families hung signs saying "Help!" "I want to live!" RFK Jr fueled the anti-vaxx mvt there & now he's in a much more powerful position here. www.nytimes.com/2019/12/19/w...

‘Why My Baby?’: How Measles Robbed Samoa of Its Young (Published 2019)

When the virus arrived on its shores, the Pacific island nation was grievously unprepared. It had left the door to contagion wide open, and thousands of children have suffered.

nytimes.com