Patrick Walsh in the @ericjoyce.bsky.social lab defends his thesis tomorrow!

We’re excited to share that, after receiving many requests each year from the research community for Oligopaint DNA & RNA FISH probes, we’re piloting a service to design & prepare probes for labs. Please share with colleagues who may benefit! ericjoycelab.com/oligopaint-f...

Oligopaint Probe Requests

In an effort to make Oligopaint FISH probes more broadly available to the research community, we offer DNA and RNA FISH probe design, synthesis, and generation. By dedicating a full-time staff memb…

ericjoycelab.com

New preprint with @gfudenberg.bsky.social We find the rate of cohesin loop extrusion in cells is set by NIPBL dosage and tunes many aspects of chromosome folding. This provides a molecular basis for NIPBL haploinsufficiency in humans. 🧵👇 www.biorxiv.org/content/10.1...

NIPBL dosage shapes genome folding by tuning the rate of cohesin loop extrusion

Cohesin loop extrusion is a major driver of chromosome folding, but how its dynamics are controlled to shape the genome remains elusive. Here we disentangle the contributions of the cohesin cofactors ...

biorxiv.org

🚨New preprint from the lab🚨 🧬 What keeps certain chromatin domains anchored at the nuclear periphery? Our new genome-wide HiDRO screen uncovers a key role for RNA-binding protein hnRNPK. www.biorxiv.org/content/10.1...

Mechanistic and Epigenetic Partitioning of Lamina-Associated Chromatin Revealed by a Genome-Wide Imaging Screen

The nuclear periphery is a key site for heterochromatin organization in eukaryotic cells, where lamina-associated domains (LADs) promote transcriptional repression and genome stability. Despite their ...

biorxiv.org

Dr. Randi Isenhart has a new 1st author publication for her work in the @ericjoyce.bsky.social lab on: The length and strength of compartmental interactions are modulated by condensin II activity: dx.plos.org/10.1371/jour...

The length and strength of compartmental interactions are modulated by condensin II activity

Author summary The three-dimensional organization of the genome plays a critical role in regulating cellular function, yet the mechanisms that govern large-scale chromatin architecture during…

dx.plos.org

ft. @HarkeJailynn, Jeewon Lee, @SonCNguyen, Staci Rakowiecki, @rebecca_yunker, @epsteind.bsky.social, @ericjoyce.bsky.social (@penngenetics.bsky.social), Arian Arab, @melikel.bsky.social (@pennepigenetics.bsky.social) & @SHugelier (@UPennPhysiology) @MolecularCell www.cell.com/molecular-ce...

two excellent papers on enhancer-promoter distance. Revisiting a classic unsolved problem. www.cell.com/molecular-ce... www.cell.com/molecular-ce... Wysocka and @chribue.bsky.social labs

Enhancer cooperativity can compensate for loss of activity over large genomic distances

Thomas, Feng, et al. introduce a synthetic platform that allows the building of complex regulatory landscapes. Integrating the same enhancer at different distances from a promoter uncovered that activ...

cell.com

🚀 Excited to share that my postdoc work with @adernburg.bsky.social on a Piezo-dependent meiotic checkpoint in C. elegans has just been published in Science doi.org/10.1126/scie... Here's a summary thread, or a “Skydive” (coined by @harmitmalik.bsky.social‬), of the key findings in this work 🧵⬇️1/10

Chemically induced proximity reveals a Piezo-dependent meiotic checkpoint at the oocyte nuclear envelope

Sexual reproduction relies on robust quality control during meiosis. Assembly of the synaptonemal complex between homologous chromosomes (synapsis) regulates meiotic recombination and is crucial for a...

doi.org

Very happy to see this collaborative work with Eric Joyce and Doug Epstein labs out in press. Happy to have contributed our small part. Congratulations to Jailynn, who recently joined my lab as a postdoc, and all co-authors on this tour de force!

Doug Epstein@epsteind.bsky.social · 2y ago

Our study with @ericjoyce.bsky.social led by Jailynn Harke is out. We show that allelic configurations at the Shh locus are predominately compact in the mouse forebrain, and that long range E-P interactions rely on transcription dependent and independent mechanisms. www.cell.com/molecular-ce...

Our study with @ericjoyce.bsky.social led by Jailynn Harke is out. We show that allelic configurations at the Shh locus are predominately compact in the mouse forebrain, and that long range E-P interactions rely on transcription dependent and independent mechanisms. www.cell.com/molecular-ce...

Multiple allelic configurations govern long-range Shh enhancer-promoter communication in the embryonic forebrain

Harke et al. use sequential DNA-FISH to achieve single-allele resolution of the Sonic hedgehog (Shh) regulatory domain in the mouse forebrain. They show that multiple allelic configurations occur inde...

cell.com