Melike Lakadamyali
@melikel.bsky.social
Professor, Physiology Department, Perelman School of Medicine, University of Pennsylvania. https://www.lakadamyali-lab.com Super resolution microscopy, single molecule biophysics, microtubules, organelles, nucleus and chromatin
🔬 #JobAlert Interested in doing a PhD with us in #SyntheticBiology on the Reconstitution of Oscillatory Microtubule-Motor Systems? Check shorturl.at/wis5P (note the eligibility criteria) or/and get in touch with us shorturl.at/WmgOD. Deadline: 31 July 2026 #WeAreHiring #AcademicJobs #ScienceJobs
Very proud of Dr. Kim on a stellar defense. This is a bittersweet moment for the lab as she will be missed. But a bright future awaits her in her new career and we all wish her the best of luck. Her contributions will continue to make an impact in our science and the field for many years to come!
After some good funding news, we are hiring! Looking for a postdoc to join our team and lead our explorations into transposon repression by the nuclear lamina. Check out the full ad on our lab website at buchwalter-lab.github.io/postdoc-ad.pdf + contact me to learn more!
🤩We're really looking forward to our next Rudolf Mößbauer Colloquium with Melike Lakadamyali @melikel.bsky.social from @upenn.edu University of Pennsylvania School of Medicine! 🚀🔬 #mmimr #maxplanck #rmc #scientifictalks #superresolution #imaging
I am so happy to see our paper on spatial feature based analysis of chromatin states in print in Nature Communications. Here is an early access version of O-SNAP: www.nature.com/articles/s41...
O-SNAP uncovers nanoscale chromatin remodeling in dedifferentiation and stress responses - Nature Communications
Changes in nuclear architecture during cell state transitions remain poorly understood. Here, the authors use O-SNAP, a framework to analyze SMLM images of chromatin, to reveal chromatin remodelling i...
nature.com
Heterogeneity of late #endosome/#lysosomes shown by multiplexed DNA-PAINT imaging. Quantitative pipeline developed by Charles Bond, Melike Lakadamyali @melikel.bsky.social and colleagues: rupress.org/jcb/article/... 📕 Part of #Lysosomes 2026: rupress.org/jcb/collecti...
Proud PI moment. Adriana’s thesis work in my lab was tour de force and I am so happy and proud to have had the privilege of hooding her today.
Cells are not just bags of molecules. Function depends on spatial interactions: RNAs and/or proteins come together with interaction partners to drive regulation and function. In our new preprint, we introduce ProPER, a novel method for in situ proximity detection. Preprint: tinyurl.com/muju86s6
Jorge Trojanowski@jorgetrojanowski.bsky.social · 3mo ago1/ ProPER - Our novel ligation-free proximity detection method for efficient multiplexed imaging of molecular interactions in situ. Among other things we use it to image the life cycle of RNA molecules and resolve differential kinetics of gene expression steps. New preprint: doi.org/10.64898/202...
Don't miss the #GordonConference on 𝑺𝒊𝒏𝒈𝒍𝒆-𝒎𝒐𝒍𝒆𝒄𝒖𝒍𝒆 𝑨𝒑𝒑𝒓𝒐𝒂𝒄𝒉𝒆𝒔 𝒕𝒐 𝑩𝒊𝒐𝒍𝒐𝒈𝒚, organized by #KevinWelsher & me: ⏰ June 28 - July 3, 2026 🌍 in the Swiss Alpes (✈️ Geneva) ✅ APPLY now: deadline May 31. We're 75% booked: register today & be there! 🙌 🕳️ 🧬🧪🧫 🔬🟩🟥🟩🟥🏋️♀️💥🪤 🍩 ... you get it... 😁
We're super excited about the speaker line-up at this GRC, featuring e.g. keynotes from @melikel.bsky.social, #ShuoHuang, #ScottBlanchard, @ceesdekker.bsky.social – and more talk slots are still available! www.grc.org/single-molec...
2026 Single Molecule Approaches to Biology Conference GRC
The 2026 Gordon Research Conference on Single Molecule Approaches to Biology will be held in Les Diablerets, Vaud (fr) Switzerland. Apply today to reserve your spot.
grc.org
What if cells have undiscovered methods for managing their internal logistics? Our recent work (@cathygalbraith.bsky.social, James A. Galbraith, @sciencethecat.bsky.social & myself) challenges existing views on intracellular transportation 🔗 lnkd.in/dhMDgY5Q
🚨 Postdoc in 3D genome architecture – join the @jumanaaha.bsky.social lab at the MPI-IE, Freiburg (🇩🇪) The wonderful lab of Jumana AlHaj Abed is pushing the limits of how chromosomes fold and interaction – and we need someone who can code, image, & think across scales. Apply here: s.gwdg.de/9c8838
Spectral multiplexing is typically limited to 4-5 channels. Our new preprint introduces a framework that utilizes DNA barcoding and signal tuning to enable robust spectral unmixing and ground-truth benchmarking to achieve 15-plex subcellular profiling without cycling www.biorxiv.org/content/10.6...
Kristina@jekristina.bsky.social · 5mo agoEver felt constrained by the "4-color limit" in fluorescence imaging? We use DNA-barcoded labeling to build high-plex panels, enabling imaging of 15 targets across the full fluorescence spectrum. doi.org/10.64898/202... Grateful to @sinemsaka.bsky.social and all authors for making this possible.
@mukherja.bsky.social work on RNAPII clusters is in print today: www.nature.com/articles/s41... Congrats! TLDR: RNAPII clusters represent transcriptonally engaged molecules at single genes, not super-stochiometric assemblies. Please check it out and see the the thread below for a summary.
A single cluster of RNA Polymerase II molecules is stably associated with active genes - Nature Communications
Live molecular-scale imaging in fly embryos shows that a single RNA Polymerase II cluster stays bound to active genes during transcription. Cluster formation requires initiation, and intensity represe...
nature.com
Apratim (Appy) Mukherjee@mukherja.bsky.social · 11mo ago(1/3) Excited to post our updated pre-print! We show RNAPII clusters are simply collections of polymerase molecules stably engaged at single genes & cluster intensity is strongly correlated with txn output during Drosophila embryogenesis. www.biorxiv.org/content/10.1...
Baby KJ update! 🧪 He's looking so good now 🥹 Hope this little guy continues to thrive and that the team at CHOP gets to save more kids like him.🫶 youtu.be/dMY-nYMeis4?...
Baby KJ One Year Later: Gene-Editing Breakthrough Update
YouTube video by The Children's Hospital of Philadelphia
youtu.be
This Friday (today), we’re pleased to welcome Melike Lakadamyali (@melikel.bsky.social) from @upenn.edu. If you are interested in Super resolution microscopy, cytoplasmic and nuclear organization with a focus on biophysics, then do not miss Prof. Lakadamyali talk in our lecture hall at 13:00. 🤗
Melike Lakadamyali @melikel.bsky.social, Jérôme Solon and Manuel Mendoza @longchrom.bsky.social share the behind the paper story of their research interrogating how chromosome compaction scales with cell size using a combination of live confocal microscopy and 3D STORM.
Seeing Chromatin Scaling with Cell Size, from Interphase to Mitosis - FocalPlane
Seeing Chromatin Scaling with Cell Size, from Interphase to Mitosis -
focalplane.biologists.com
Thanks to @focalplane.bsky.social for the invitation to highlight our recent @biophysj.bsky.social paper! With @melikel.bsky.social and Jérôme Solon, we combined super-resolution and live imaging to connect interphase chromatin organisation to mitotic chromosome compaction across cell sizes.
Seeing Chromatin Scaling with Cell Size, from Interphase to Mitosis - FocalPlane
Seeing Chromatin Scaling with Cell Size, from Interphase to Mitosis -
focalplane.biologists.com
EMBL is deeply saddened by the passing of our Interim Director General Peer Bork, a pioneering scientist who left an indelible imprint on life science research in Europe and beyond. We extend our deepest condolences to his family, friends, and colleagues. www.embl.org/news/people-...
So proud to watch Jana turn a side quest into an entire research programme. Couldn’t imagine a more well-deserved outcome. 😍 The mighty yeast reveals what we believe are general principles of centromere evolution! Behind the curtain: communities.springernature.com/posts/counti... #MEvoSky 🧪🌎
Counting Chromosomes: The Simple Idea That Unlocked the Rules Behind Centromere Evolution
communities.springernature.com
Jana Helsen@helsenjana.bsky.social · 8mo agoHow do new centromeres evolve while staying compatible with the division machinery? Discover it in our new Nature paper! We show centromeres transition gradually via a mix of drift, selection, and sex, reaching new states that still work with the kinetochore. 👉 doi.org/10.1038/s41586-025-09779-1
Last call for Les Houches! Apply now or never! ;) (ok, in 2 years again then) and share with your friends.
Ulrike Endesfelder@uendesfelder.bsky.social · 11mo agoPlease share: The application for Les Houches is open (until Oct 31) fluorescenceleshouches.wordpress.com *This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!* PS: no fees this year, you just need to get there, that's all!
Job alert! We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks? See also on the forum: forum.image.sc/t/research-e...
Research engineer in Bioimage Analysis for the researchers of the inIdEx FORMULA
Hi all We are looking for a bioimage analysis to work in an image analysis core facility in beautiful Paris. Can I ask you to share this opportunity with your networks? The position is in the Instit...
forum.image.sc
How do #stemcells integrate information to coordinate fate decisions? Delighted to finally see our work showing how growth factors regulate the mechano-osmotic state of the #nucleus and #chromatin to control #pluripotency exit out! www.nature.com/articles/s41... see 🧵 👇
Mechano-osmotic signals control chromatin state and fate transitions in pluripotent stem cells - Nature Cell Biology
McCreery, Stubb et al. show that mechano-osmotic changes in the nucleus induce general transcriptional repression and prime chromatin for cell fate transitions by relieving repression of specific differentiation genes.
nature.com
Cornelis (Cees) Dekker to Receive the 2026 Kazuhiko Kinosita Award in Single-Molecule Biophysics buff.ly/fIexyZu
Please share: The application for Les Houches is open (until Oct 31) fluorescenceleshouches.wordpress.com *This is for you, if you ever wanted to learn all about fluorescence markers & how to apply them for your biological research!* PS: no fees this year, you just need to get there, that's all!
French-German WE-Heraeus-Seminar "Fluorescence markers for advanced microscopy : From photophysics to biology - 2026"
March 15-20 2026, Les Houches, France
fluorescenceleshouches.wordpress.com
