Ever wondered how mammalian cells sense osmotic stress? There's gotta be a signaling pathway, right? We propose a *one-component system*: NFAT5, the transcription factor that executes the osmoprotective response, is also the stress sensor! www.science.org/doi/full/10....

Direct ionic stress sensing and mitigation by the transcription factor NFAT5

Intracellular ionic strength is sensed and regulated by a prion-like domain in a transcription factor.

science.org

It’s been a tough few weeks. My 10yo daughter was diagnosed with a very rare, aggressive cancer called interdigitating dendritic cell sarcoma (IDCS). I’m reaching out to identify clinicians/patients who have encountered pediatric IDCS or other (non-LCH) dendritic or histiocytic sarcomas cases.

Quick poll: what year is the below abstract from? „Our results indicate that protein sequences contain not only a folding code, but also a previously unrecognized code governing their distribution to diverse subcellular compartments.“

Dunno what they have planned, but a devastated NIH will destroy universities and academic science (on the heels of significant COVID impacts) but will also destroy biotech and cede US leadership in this sector to China and elsewhere very very quickly.

The Dept. of Biochem. & Biophys. @ WashU is running its summer undergrad research program! Rising seniors who have not had the opportunity to do bona fide research but are interested in this are strongly encouraged to apply Deadline: Jan 31st Details: biochem.wustl.edu/resources/in... Pls share!

Proud to share our work spear-headed by PhD student Tommy Sroka with major contributions by #xenopus expert Kerstin Feistel. Our iAPEX #ProximityLabeling method for #MassSpec based subcellular #proteomics works by locally generating H2O2 using a D-amino acid oxidase that activates APEX2 in situ.

iAPEX: Improved APEX-based proximity labeling for subcellular proteomics using an enzymatic reaction cascade

Ascorbate peroxidase (APEX) is a versatile labeling enzyme used for live-cell proteomics at high spatial and temporal resolution. However, toxicity of its substrate hydrogen peroxide and background la...

biorxiv.org

Found some old drawings I had to do for my undergrad in Molecular & Cellular Biology. @dadrummond.bsky.social may particularly appreciate this one, although I’m not nearly the artist he is.

@boeynaemssteven.bsky.social New night science project?

Pawel Burkhardt@pawelburkhardt.bsky.social · 2y ago

Did much of the basic tool kit for multicellular development, possibly even animal development, arose early in eukaryote evolution? The Acrasis kona genome and developmental transcriptomes reveal deep origins of eukaryotic multicellular pathways. www.nature.com/articles/s41...