Hiroshi Abe (べべひろ)

@hirabe.bsky.social

Studying ecology and conservation of ectomycorrhizal fungi🍄 with 2 leopard geckos🦎 Shoro-chan and Alpova-chan💕 Tweet mainly in Japanese. Opinions are my own. きのこ好き。レオパ。菌根菌 レオパのショウロちゃんとアルポバ氏ときのこの研究をしています🍄

So excited to share that one of the chapters from my thesis has been published! In it we discovered that, despite previous assumptions, Escovopsis (a mycoparasite in the fungus growing ant symbiosis), has a complete MAT locus under purifying selection.

Genome Biology and Evolution@genomebiolevol.bsky.social · 2w ago

@soleilsolay.bsky.social et al. find that Escovopsis s.l. - specialized fungal parasite of the fungus-growing ant symbiosis - likely undergoes cryptic sex as all examined strains have a complete mating-type locus under purifying selection. 🔗 doi.org/10.1093/gbe/evag160 #evolution #societyjournal

GBE | Evidence for Cryptic Sex in Escovopsis, a Mycoparasite in the Fungus-Growing Ant Symbiosis

「MIG-seq2:多数のデュアル・ユニーク・インデックスの利用が可能になったMIG-seq法の改良版」 #OpenAccess

Ecological Research@ecologicalresearch.bsky.social · 3w ago

<TECHNICAL NOTE> #OpenAccess MIG-seq2: Multiplexed Inter-Simple Sequence Repeat Genotyping by Sequencing With Dual-Unique Indices Yoshihisa Suyama, Naoko Ishikawa 👉 doi.org/10.1111/1440... genome-wide SNP analysis/ MIG-seq/ molecular phylogenetics/ phylogeography

Graphical abstract: We developed a new version of the Multiplexed ISSR Genotyping by Sequencing (MIG-seq) method, called MIG-seq2. This improved protocol incorporates dual unique indices to distinguish more than 1344 samples and optimizes primer tail sequences to improve sequence quality. Additionally, we designed an optional degenerate primer set (dpMIG-seq2) to enhance amplification efficiency in low-diversity samples. The effectiveness of these improvements was confirmed by phylogenetic analyses of two closely related Plantago species, which yielded highly reproducible and well-supported trees.