Tessera

Imtiyaz Hariyani

@imtiyazh.bsky.social

PhD Candidate at UC Irvine. Evolutionary genomics, transcriptome evolution, single-cell, transposable elements, Drosophila.

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Imtiyaz Hariyani @imtiyazh.bsky.social · 3w ago

My first first-author paper from my PhD is out! Check it out in @plosbiology.org

PLOS Biology@plosbiology.org · 3w ago

Reproductive organs from different species contain conserved cell types. This study compares single-cell data of two Drosophila species, showing that some testis cell types are hotspots for gene expression #evolution, while ovarian cells are more conserved. 🧪 #AcademicSky plos.io/4hqIkMd

Magnitude of expression differentiation across testis and ovary cell types at two evolutionary timescales.

Venn diagrams showing the number of differentially expressed genes (DEGs) unique to and shared between D. melanogaster and D. simulans in the testis: (A) at the inter- and intraspecific levels; and (B) across the three pairwise comparisons involving the D. melanogaster strains A4 and ISO1, and the D. simulans strain w501. (C) Patterns of differential expression across pairwise comparisons and testis cell types. Strict Inter (pattern 110): DEGs in both interspecific contrasts in the same cell type without being differentially expressed at the intraspecific level. Strict Intra (pattern 001): DEGs at the intraspecific level without being differentially expressed at the interspecific level. (D) Percentage of DEGs per testis cell type between the D. melanogaster strains A4 and ISO1, shown relative to the D. simulans strain w501. DEGs are categorized based on whether they show consistent differential expression in both interspecific comparisons (A4 vs. w501 and ISO1 vs. w501) or are unique to only one of the comparisons. (E–H) Equivalent plots for the ovary. Genes were required to be expressed in at least 1% of cells with a minimum average expression of 0.01. Asterisks, cell types showing the lowest and highest deviation relative to the random expectation for DEGs consistent across both interspecific contrasts. Testis cell types: EC, epithelial cells; HC, hub cells; CC, cyst cells; ES, germline stem cells and early spermatogonia; LS, late spermatogonia; ESp, early spermatocytes; MSp, mid spermatocytes; LSp, late spermatocytes; MPSp, maturing primary spermatocytes; and S, spermatids. Ovary cell types: GSC/G1-2a, germline stem cells and germarium region 1 and 2a cells; G2a-2b, germarium region 2a and 2b cells; G2b-3, germarium region 2b and 3 cells; SPC, stalk and polar cells; FSC/preFCs, follicle stem cells and pre-follicle cells; EFC, early follicle cells; MFC, mito

Imtiyaz Hariyani @imtiyazh.bsky.social · 2mo ago

If you’re at #PEQG26, come check out my poster tonight on transposable element expression in the Drosophila testis, resolved at single-cell level! PgmNr: 387T Cell-type- and locus-resolved transposable element expression during Drosophila spermatogenesis