A beautiful journey through the nuclear pore complex with #cryoET - samples prepared via the Arctis Cryo-Plasma-FIB, data collected with the Glacios Cryo-TEM and segmented in Amira Software. #TeamTomo - See what’s possible with 200 kV cryo-ET: ter.li/n2a8i5po

"Why paying peer reviewers works, according to a journal’s editor-in-chief" I wonder if this is going to change anything.....old idea, new data, uncertain impact. Your thoughts? www.nature.com/articles/d41...

Why paying peer reviewers works, according to a journal’s editor-in-chief

A biology journal that paid peer reviewers found that the approach cut the time to a first editorial decision by 85% and maintained high-quality reviews.

nature.com

🔥My first (co)first-author paper is out🔥 The surfaces of Gram+ and Gram- bacteria are markedly different from each other. How are phages equipped to breach the Gram+ envelope❓ 💡We report a baseplate structure uniquely adapted to infect Gram+ S. aureus. A 🧵⬇️ 1/8 #phagesky #cryoEM 🧪

I love when real life issues get explained by science (scientists) so elegantly 👏🏻

Buttons (Ashley) 🏴‍☠️#OFMDForLife🏴‍☠️ 🏳️‍🌈🤡@sevensugars.bsky.social · 2mo ago

Since I did do my PhD in cyanobacteria growth and treatment optimization modeling AND work in the water treatment industry, I’m gonna share my theory as to what’s happening here and how to fix it. People are focusing on the painted bottom of the pool increasing residual heat, and while that 1/19

High-Resolution #virus blueprint offers new hope against #antibiotic resistance ... | #antibioticresistance | #bacteria | #bacteriophages | #phage | By @universityofotago.bsky.social via scitechdaily .com

Stunning High-Resolution Virus Blueprint Offers New Hope Against Antibiotic Resistance

A detailed 3D map of a bacteria-hunting virus reveals ancient origins and new promise against superbugs.

scitechdaily.com

🚨Preprint! Happy to share the research from my PhD “Genome delivery of a contractile tailed phage and its superinfection exclusion mechanism”. We use cryoEM to study the genome ejection of the phage T4, revealing how the tape measure protein regulates the process. www.biorxiv.org/content/10.6...

Much needed addition to the AlphaFold Database! 💻 Large potential for use in #StructuralBiology #Phage research.

Gemma C. Atkinson@gemmaatkinson.bsky.social · last yr.

Really excited to share our resource and analysis of AlphaFold-predicted monomers and homodimers across viruses and phages. Project driven by fab PhD student @rodai.bsky.social and with our great collaborators Joana Pereira @joanampereira.bsky.social and @ninjani.bsky.social

Always happy to provide "wonderfully bad mmCIF files" to make #chimeraX better!

Nice work and awesome #scicomm thread!

Brian Kelch@briankelch.bsky.social · last yr.

Did you know that there is so much DNA packed inside a phage capsid that the pressure is 10X higher than in a bottle of champagne? In our latest preprint, we wondered how that is contained in a phage that lives at extremely high temperatures. /1 www.biorxiv.org/content/10.1...

Our study on pasteurising influenza viruses in milk is now out! It's a nice simple story: influenza viruses (including H5N1) are killed really effectively by pasteurisation, but in raw milk they stay infectious - obvious public health implications of both points... (1/2) rdcu.be/d73te

I'd like to draw your attention to this truly excellent paper from the Taraska lab on cryoET of plasma membrane associated proteins. Everyone who is thinking about probes in the cryoET space should also see what they could do with ferritag (fig 6) www.nature.com/articles/s41...

I appreciate the stats! Thanks, @pdbeurope.bsky.social for a summary!

Protein Data Bank in Europe (PDBe)@pdbeurope.bsky.social · 2y ago

As we head into 2025, let's take one final look back at 2024 and how the year unfolded in the PDB archive. There were 15319 entries released last year but what more can we find out about those structures ⚖️? A thread 1/11 🧵

I'm happy and humbled to take on a new role with @rcsbpdb.bsky.social. I'll be continuing David Goodsell's work on the Molecule of the Month, starting with my first article on Assembly Line Polyketide Synthases! Check it out (animation included!) pdb101.rcsb.org/motm/301

PDB101: Molecule of the Month: Assembly Line Polyketide Synthases

Large multienzyme complexes that synthesize diverse small molecules in a stepwise manner.

pdb101.rcsb.org

Amazing work and resource! 😍

Ben Engel@cellarchlab.com · 2y ago

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍 Preprint📜: www.biorxiv.org/content/10.1... A short thread🧵👇

SPACEtomo v1.2 is online! github.com/eisfabian/SP... - Improved GUIs for lamella detection, target selection and CLEM registration make it the best target selection workflow for PACEtomo on any kind of lamella sample. - Easier installation and configuration Any feedback is much appreciated! :)

🎄Xmas office cleaning 🗑️ ...and how do you organize your thoughts? 🫵 #PhDlife #PhDSky #mindmap

I knew it wouldn't be long before we see #cryoEM structures of a #phage cousin infecting #Archea 🦠 🧪 Beautiful structures from @daumlab.bsky.social, congrats!

The Daum Lab@daumlab.bsky.social · 2y ago

Check out our structure of the archaeal virus HFTV1! Amazing work by two students - Daniel Zhang and Becky Davies, supported by @mathewmclaren.bsky.social. Great collaboration with T. Quax & S. Schwarzer, @hanna-m-oksanen.bsky.social, @the-gold-lab.bsky.social, @misha-isupov.bsky.social & W. Stuart.

Dear #StructuralBiology #PhD #PostDoc peers, join in!

Razvan Borza@rborza.bsky.social · 2y ago

I created this Starter Pack for PhDs and Postdocs in Structural Biology to help connect with colleagues who share similar interests and concerns in the field. Feel free to recommend people or yourself! I'll be adding people 💪🏼😁 bsky.app/starter-pack...

Another useful starter pack 👇

Kate Michie@kmichie.bsky.social · 2y ago

Structural Biology Starterpack go.bsky.app/DDetSBp I like feeds better as it filters out 'cat' talk, leaves in the selective structural biology chitchat (Protein Cosmos). Poke me for inclusion/removal. Apologies if I've broken etiquette and included/excluded you first up (not intended to offend).

In case someone is looking for this...

Rémi Fronzes@fronzeslab.bsky.social · 2y ago

Here is a starter pack for CryoEM/ET enthousiasts To be completed go.bsky.app/JmQksC

Just uploaded a revised version of the manuscript on bioRxiv 🙌 We made some changes to the text and figures, mainly to clarify the naming of proteins. Yeah, it's a challenge to describe things within two different 10MDa structures 🐘

Ján Bíňovský@jbinovsky.bsky.social · 2y ago

🚀Announcing my first (co)first-author manuscript 🚀 "Cell attachment and tail contraction of S. aureus phage phi812" 🔗 bit.ly/Phi812 Explore the structural details of phage interaction with Gram+ bacteria 🤯 #PhageTherapy #cryoEM 🧪 | 🧬🧶 | 🦠 1/11

🚀Announcing my first (co)first-author manuscript 🚀 "Cell attachment and tail contraction of S. aureus phage phi812" 🔗 bit.ly/Phi812 Explore the structural details of phage interaction with Gram+ bacteria 🤯 #PhageTherapy #cryoEM 🧪 | 🧬🧶 | 🦠 1/11

Cell attachment and tail contraction of S. aureus phage phi812

bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution

bit.ly

Reading a lot about contractile phages and injection systems, the idea of leveraging them for cargo delivery always amazed me 🤯 and it's no longer just an idea 🧪 Here, a T. gondii secretion system was hijacked to deliver therapeutic proteins to neurons. Link to study: rdcu.be/dQozB

Engineering Toxoplasma gondii secretion systems for intracellular delivery of multiple large therapeutic proteins to neurons

Nature Microbiology - Toxoplasma gondii secretion systems can be engineered to deliver multiple large therapeutic proteins to neurons in vitro and in vivo.

rdcu.be

Using MS Word's "Dictate" function these days: "I did an internshit..." "I learned to cry electron microscopy" ...how am I supposed to write a motivation letter with this? 😢

Really good 15 min TWiV episode with Paul Offit outlining how false and irresponsible the recent lab leak nonsense from @nytimes.com is. I for one appreciate the brevity - there really is nothing more to it. If you're interested in the topic, give it a listen. www.youtube.com/watch?v=Ukv9...

Beyond the Noise #40: Lab leak mania

In this episode Dr. Offit asks, why did the New York Times publish an op-ed supporting the lab leak theory when all evidence supports a zoonotic spillover?Sh...

youtube.com

Manuscript submitted 🤞 Brace for new structural insights into a bacteriophage landing onto the host cell 🚀

📰 I find myself questioning my scientific career from time to time. This is an encouraging piece. "Meaningfulness in a scientific career is about more than tangible outputs." www.nature.com/articles/d41...

Just finished registration for the Gordon Research Seminar (GRS), complementing the conference (GRC) on 3D #ElectronMicrocopy 🔥 Looking forward to attending the GRC/S for the first time! Am I gonna meet someone from my BlueSky bubble? 🙌

📰 Some positive news for the afternoon reading: a survey about trust in scientists. "...trust levels are fairly high across a range of demographics." "It’s about the leadership of political parties and how they treat scientists." www.nature.com/articles/d41...

Largest post-pandemic survey finds trust in scientists is high

Study of more than 70,000 people suggests that trust levels vary among countries and are linked to political orientation. Study of more than 70,000 people suggests that trust levels vary among countri...

nature.com

I am excited to share that my work with @cijilim.bsky.social on protecting fragile samples from air-water-interface damage during cryo-EM plunge freezing is in preprint! www.biorxiv.org/content/10.1...