Large extrachromosomal replicons are widespread across bacterial lineages and show coordinated replication termination and spatial coupling with the chromosome - Nature Communications

Bacterial genomes often include extrachromosomal replicons, ranging from small plasmids to chromosome-scale replicons. Here, Czarnecki et al. analyse more than 40,000 complete bacterial genomes and sh...

doi.org

Role of endopeptidases in lateral cell wall expansion in Escherichia coli

The Escherichia coli bacillus morphology is thought to be determined by the shape of the peptidoglycan macromolecule, although the underlying polymerization mechanism remains elusive. Anoyatis-Pelé et al. show that lateral cell wall expansion at a constant diameter depends upon the endopeptidase-dependent insertion of single glycan strands into the peptidoglycan mesh.

dlvr.it

Taming wild replicons: evolution and domestication of large extrachromosomal replicons

Bacterial genomes often contain extrachromosomal replicons (ERs), ranging from small, mobile plasmids to large, stably inherited elements, such as meg…

sciencedirect.com

Staphylococcus aureus exploits lipoic acid salvage to combat host oxidative stress

Acosta et al. find that ADP-ribosylation of the lipoic acid carrier protein GcvH-L promotes Staphylococcus aureus resistance to oxidative burst and facilitates infection. ADP-ribosylation leads to a structural shift within GcvH-L that safeguards lipoic acid sulfhydryls from detrimental oxidation and regulates its transfer to enzyme complexes required for growth.

dlvr.it

Streptomyces uses both polar and dispersed cell wall synthesis during exploratory growth - Nature Microbiology

Streptomyces venezuelae requires MreB1-dependent dispersed peptidoglycan cell wall synthesis and DivIVA-mediated polar cell wall synthesis during its rapid exploratory growth phase.

nature.com

SpoIIIL is a forespore factor required for efficient cell-cell signalling during Bacillus subtilis sporulation

Author summary Bacterial endospores allow bacteria to survive stress, persist in the environment and seed new and recurring infections. Cell-cell signalling is a cornerstone of how bacteria develop in...

dx.plos.org

Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria - Nature Microbiology

Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and counter cytoplasmic turgor pressure, preventing lysis during osmotic challenges in Escherichia coli.

nature.com

Optimization of Prodrug Activation by Enzymatic Cleavage of the β‐lactam Ring of Carbapenems

We report synthetic routes that yield prodrugs consisting of two carbapenems connected by benzyl carbonate linkers. A mass spectrometry assay was elaborated to monitor, in a single kinetic experiment...

doi.org

An inner membrane protein is covalently attached to peptidoglycan in the γ-proteobacterium Dickeya dadantii

Communications Biology - In Gram-negative bacterium Dickeya dadantii, an inner membrane protein OutB is covalently attached to peptidoglycan by the same catalytic mechanism as Braun’s...

rdcu.be

Mechanism of lateral cell-wall expansion at a constant diameter in Bacillus subtilis

Nature Communications - In Escherichia coli, lateral cell-wall expansion during growth occurs by crosslinking of new glycan chains to the existing peptidoglycan network. Here, Liang et al. show a...

rdcu.be

The addition of amoxicillin improves the efficacy of the imipenem-avibactam combination against Mycobacterium abscessus in a mouse model of infection | Antimicrobial Agents and Chemotherapy

Mycobacterium abscessus is a rapidly growing mycobacteria responsible for pulmonary diseases in patients suffering either from chronic obstructive pulmonary diseases or cystic fibrosis (1, 2). Smooth ...

journals.asm.org

In vitro, intracellular and in vivo synergy between amoxicillin, imipenem and relebactam against Mycobacterium abscessus

AbstractObjectives. Mycobacterium abscessus is the most frequent of the rapidly growing mycobacteria responsible for lung infections in patients suffering

doi.org

Pre-registration and Abstract Submission - The Greatwall-symposium

Registration and abstract submission open on January 15th, 2025.The organizers will select the final list of participants based on abstract submission, particularly for young scientist, and the applic...

thegreatwall-symposium.org

Peptidoglycan-tethered and free forms of the Braun lipoprotein are in dynamic equilibrium in Escherichia coli

The Braun lipoprotein is tethered to peptidoglycan independently from the insertion of newly-synthesized peptidoglycan subunits into the expanding cell wall generating a dynamic equilibrium between fr...

elifesciences.org

(p)ppGpp modifies RNAP function to confer β-lactam resistance in a peptidoglycan-independent manner - Nature Microbiology

The bacterial alarmone (p)ppGpp induces β-lactam resistance through modification of RNA polymerase and ribosome function rather than regulation of peptidoglycan metabolism in Escherichia coli.

nature.com

In vitro and intracellular activity of vaborbactam combined with β-lactams against Mycobacterium abscessus

AbstractObjectives. Mycobacterium abscessus has emerged as an opportunistic pathogen responsible for lung infections, especially in cystic fibrosis patient

academic.oup.com

Monitoring Drug–Protein Interactions in the Bacterial Periplasm by Solution Nuclear Magnetic Resonance Spectroscopy

The rapid spread of antimicrobial resistance across bacterial pathogens poses a serious risk to the efficacy and sustainability of available treatments. This puts pressure on research concerning the d...

pubs.acs.org