(1/5) Individual mitochondrial cristae with a 10X air lens 🤯 I am very happy to be a co-author on Mega-ExM! Driven by @igvegvas.bsky.social at @sauer-lab.bsky.social . It is really exciting to see how far Ignacio has pushed the technique since we learned it together back in 2019.

(4/4) Mega-ExM starts to bring ultrastructural detail in cells toward what was previously limited to cryo-ET, while keeping fluorescence microscopy flexibility. www.biorxiv.org/content/10.6... Hope you find it interesting—and thanks to everyone involved! #FluorescenceFriday #MegaExM

Structural cell biology by mega-expansion microscopy

Structural characterization of macromolecular assemblies within intact cells remains one of the central challenges in cell biology. While cryo-electron tomography provides unparalleled structural info...

biorxiv.org

(1/4) We are very excited to share our latest preprint from @sauer-lab.bsky.social: Mega Expansion Microscopy (Mega-ExM)! We combine iterative hydrogel expansion with whole-proteome NHS labeling and post-expansion immunostaining, reaching tunable expansion factors up to ~1500× in whole cells 🤯

bioRxiv Biophysics@biorxiv-biophys.bsky.social · 2mo ago

Structural cell biology by mega-expansion microscopy https://www.biorxiv.org/content/10.64898/2026.08.05.743040v1