Juan Pablo Tosar
@jptosar.bsky.social
RNA Biology & extracellular RNA. Group Leader, Functional Genomics Lab at Pasteur Institute of Montevideo / Analytical Biochemistry Unit at Universidad de la República, Uruguay.
How can a single cell learn without a brain? We explore this in my paper with @wallaceucsf.bsky.social! We discovered that single cells may learn using molecules similar to those that animal brains use to learn, like CaMKII. Cells can also propagate memory states to their progeny!
Thrilled to be featured in this month’s @rnasociety.bsky.social Spotlight! Science is a team effort, so this recognition belongs to my colleagues and students. I'm so lucky to work with them! (Yes, the #AUG1 story is here, straight from the golden age of Tw. 🐦) www.rnasociety.org/spotlight/dr...
Dr. Juan Pablo Tosar - RNA Society
One of Dr. Juan Pablo Tosar’s proudest moments came from social media, when he replied to the RNA Society’s question of whether we should have an RNA day with “AUG1”, thereby contributing to the initi...
rnasociety.org
Attention scientists from all over the world🙃 The 2026 #HFSPResearchGrants are out! 🥳 117 scientists from 31 countries will pursue bold, interdisciplinary research, from #neuroscience to #ecology and beyond! 🧪 Are you one of the new #HFSPAwardees? 🔗https://bit.ly/4uGmMQ3 #sts #HFSP
With @amyhbuck we wrote this piece on extracellular RNAs that intends not to give answers but to propose questions that can expand the field in new directions. Hope you like it! We really enjoyed a lot brainstorming it. journals.asm.org/doi/10.1128/...
mGem: Guides or triggers? Extracellular RNAs beyond vesicular miRNAs | mBio
Blood and other extracellular biofluids have a high RNase content. Hence, from its origins, the exRNA field has focused on extracellular vesicles (EVs) as the main carriers of RNA molecules that move ...
journals.asm.org
You can read the published version @pubs.acs.org here: pubs.acs.org/doi/10.1021/...
Tetraspanin Positivity as a Function of Extracellular Vesicle Size Measured by a Modified Immuno-TEM Protocol
The identification of surface markers that correlate with specific subpopulations of extracellular vesicles (EVs) is important for EV identification, classification, purification, sorting, and functio...
pubs.acs.org
Juan Pablo Tosar@jptosar.bsky.social · 2y agoNew preprint: we isolated an heterogeneous population comprising both large and small #ExtracellularVesicles. Using a modified immuno-gold protocol, we show that, at least in U-2 OS EVs, CD9 and CD81 are enriched in EVs < 100 nm. www.biorxiv.org/content/10.1...
🔥🔥🔥NEW PAPER OUT in @genomebiolevol.bsky.social with @psarkies.bsky.social and many others who are not here, yet!🔥🔥🔥 academic.oup.com/gbe/advance-...
Substantial hierarchical reductions of genetic and morphological traits in the evolution of rotiferan parasites
Abstract. Within the last 800 million years animals evolved a vast range of diversity of species exhibiting an enormous disparity of forms and lifestyles.
academic.oup.com
Check out our paper about naked extracellular RNAs uptake, TLR signaling, endosomal escape, and the role of RNases in regulating this process. www.cell.com/cell-genomic...
Ribonuclease activity undermines immune sensing of naked extracellular RNA
Castellano et al. show that when extracellular RNases are inhibited or absent, naked extracellular RNA is internalized spontaneously by cells, inducing inflammation mediated by endosomal TLRs or cytos...
cell.com
New preprint: we isolated an heterogeneous population comprising both large and small #ExtracellularVesicles. Using a modified immuno-gold protocol, we show that, at least in U-2 OS EVs, CD9 and CD81 are enriched in EVs < 100 nm. www.biorxiv.org/content/10.1...
There are concerns that Bluesky's impact has faded since the initial hype. This is false. That impression results from reading headlines about social media instead of the hard data. The five HIGHEST points of Bluesky posts mentioning research all occur in the last 2 weeks.
Altmetric@altmetric.com · 2y agoThe general trend is crazy. Here is Twitter vs Bluesky volumes for the last week. On some days they are equal in volume.
Not all tRNA fragments (tDRs) are created equal. Here we share methods to differentiate: 1) real tDRs and those produced by dissociation of nicked tRNAs, 2) nicked tRNAs / tDRs in extracellular vesicles vs outside. 1) www.sciencedirect.com/science/arti... 2) www.sciencedirect.com/science/arti...
Here's a clever new study looking at where mRNA vaccine lipid nanoparticles (mRNA-LNPs) go after vaccination (using relevant doses of vaccine) in non-human primates (and mice): www.cell.com/molecular-th... Brief thread🧵
GRC on RNA Nanotechnology came to an end yesterday. Thanks to the organizers for inviting me to present our work on the uptake of naked exRNAs, and to serve as discussion leader of the RNA Delivery session. What a fantastic and enjoyable conference! www.grc.org/rna-nanotech...
Very happy to start the new year with publication of our new work on leaf surface RNA. Plant leaves secrete diverse RNAs that are surprisingly stable on their surfaces. Next question to answer: What do they do? www.pnas.org/doi/epub/10....
"Here, we show that this Ab appears to be unreliable due to providing false-positive signals at the expected molecular weight of Bax in immunoblotting experiments and likewise provides false-positive signals in immunofluorescence-based detection of Bax expression" www.nature.com/articles/s41...
Why Bax detection in >1400 publications might be flawed - Cell Death & Disease
Cell Death & Disease - Why Bax detection in >1400 publications might be flawed
nature.com
Some of you probably do not realize how difficult it is to get a BIG international grant when located in LMIC (especially in Argentina). I am so proud of you @pstrobl.bsky.social. So well deserved. I am so happy to be part. You are the best!! @wellcometrust.bsky.social @unsamoficial.bsky.social
Pablo Strobl-Mazzulla@pstrobl.bsky.social · 2y agoThrilled to share that our project will be granted by @wellcometrust.bsky.social for the following 8 years at @IntechChascomus @unsamoficial.bsky.social I would like to thanks the collaborator of the project @ndemiguel.bsky.social and @eliasbarrigalab.bsky.social for all the support.
But please dont share this as a mantra for success. If your success comes at the direct expense of your colleagues because they are doing all the work everyone is supposed to share in, it does not make you ‘successful’ it just makes you a horrible colleague. Also see peer review Rant over. For now
This will be the next level of scientific devaluation: science to proof a political standpoint, rather than policy following scientific proof.
Alastair McAlpine, MD@alastairmca30.bsky.social · 2y agoTrump says RFK Jr will “investigate” the link between vaccines and autism as “somebody needs to find out.” We’ve ALREADY FOUND OUT - >600,000 kids in Denmark - >90,000 in the US - >1.2m kids worldwide NO LINK. How many more of these does he want us to do?
MirGeneDB 3.0 is published. Great teamwork and substantial improvement of database, Taxon sampling and we took over nomenclature of novel genes and families, too academic.oup.com/nar/advance-...
MirGeneDB 3.0: improved taxonomic sampling, uniform nomenclature of novel conserved microRNA families and updated covariance models
Abstract. We present a major update of MirGeneDB (3.0), the manually curated animal microRNA gene database. Beyond moving to a new server and the creation
academic.oup.com
First post, last paper out www.frontiersin.org/journals/tub... @carlos-robello.bsky.social
Frontiers | Serum-derived host miR-423-5p and miR-378a-3p as molecular markers for severe tuberculosis: a promising prognostic tool for survival
Tuberculosis (TB) remains a leading cause of infectious disease-related mortality. Annually, 10 million people contract TB, resulting in 1.5 million deaths, ...
frontiersin.org
The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1
The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1
Gene expression programs underpin the development of shared phenotypes, yet the importance of transc
biorxiv.org
📢SAVE THE DATE📢 The 4th Molecular Biosystems Conference is set! Plan to join us in southern Chile for insightful discussions on gene regulation & functional genomics #mbiosys25 🧬🖥️ Stay tuned for more details! 🗓️Sep 29-Oct 3, 2025 📍Puerto Varas, Chile 🌐http://molbiosystems.com
