Now, synthetic enhancers with single cells - great work by @juruehle.bsky.social : www.biorxiv.org/content/10.6...

Lars Velten@larsplus.bsky.social · last yr.

Out in Cell @cp-cell.bsky.social: Design principles of cell-state-specific enhancers in hematopoiesis 🧬🩸 screen of fully synthetic enhancers in blood progenitors 🤖 AI that creates new cell state specific enhancers 🔍 negative synergies between TFs lead to specificity! www.cell.com/cell/fulltex... 🧵

The main project of my PhD 🧬🔬 is out: we developed single-cell lentiMPRA, a lentivirus-based method to measure enhancer activity and transcriptomes at single-cell resolution. We then applied sc-lentiMPRA to fully synthetic enhancers 🧩... 🔗 doi.org/10.64898/202...

Researchers from the @crg.eu, the @irbbarcelona.org, and the DKFZ have discovered that the reservoir of blood stem cells shrinks with #age. It becomes increasingly dominated by stem cells that produce pro-inflammatory immune cells. t1p.de/kjf02 👇

How aging changes the blood system in humans and mice

The reservoir of blood stem cells shrinks with age. It becomes increasingly dominated by stem cells that produce immune cells associated with chronic inflammation. Almost all of the 60-year-olds studi...

t1p.de

Out @nature.com: Clonal tracing with somatic epimutations 🧬 Single cell methylome encodes cell state & clonal identity 🔨 EPI-Clone reads out both (+mutations, +RNA) at scale 🩸 Clonal expansions of HSCs are universal from age 50, not driven by CH mutations doi.org/10.1038/s415... 🧵

Clonal tracing with somatic epimutations reveals dynamics of blood ageing - Nature

The discovery that DNA methylation of different CpG sites can serve as digital barcodes of clonal identity led to the development of EPI-Clone, an algorithm that enables single-cell lineage tracing th...

doi.org

🧬🔍How can enhancers achieve tissue-specific activity? We use MPRAs of synthetic enhancers to derive interpretable rules on TFBS arrangement 🚦 and discover that negative synergies drive specificity in hematopoiesis 🩸. Shoutout to @Robert Frömel & @larsplus.bsky.social for leading this work 🦹🦸.

Lars Velten@larsplus.bsky.social · last yr.

Out in Cell @cp-cell.bsky.social: Design principles of cell-state-specific enhancers in hematopoiesis 🧬🩸 screen of fully synthetic enhancers in blood progenitors 🤖 AI that creates new cell state specific enhancers 🔍 negative synergies between TFs lead to specificity! www.cell.com/cell/fulltex... 🧵

Honored to be able to speak among such an amazing line-up of speakers. Check out the Single Cell Genomics Day 2025 if you want to learn more about DNA methylation based lineage tracing: satijalab.org/scgd25/