🚨 Want to do your PhD or postdoc with us? Taking candidates for @GVA APOSTD (postdoc) & CIACIF (predoc) applications 🧬 long reads · single-cell & spatial · multi-omics · AI for biology 🧑‍⚕️🐭🐙🌍 human disease · model & non-model species · One Health 📩 conesalab@gmail.com, don't wait until the deadline!

Registrations are now open for the next edition of the @nanoporetech.com direct #RNAseq with Leda Katopodi in December (14-17). @longtrec.bsky.social @crg.eu @evamarianovoa.bsky.social www.physalia-courses.org/courses-work... #LongReads

Nanopore direct RNA Sequencing

Dates 14-17 December 2026 To foster international participation, this course will be held online

physalia-courses.org

🎉 Our TUSCO paper is out in Nature Communications! A new benchmarking framework for long-read transcriptome reconstruction using curated single-isoform genes as internal ground truth — no spike-ins needed, by talented @tianyuanliu.bsky.social 🧬🔗 nature.com/articles/s41467-026-72089-1

TUSCO: benchmarking transcriptome reconstruction with endogenous single-isoform controls - Nature Communications

Long-read sequencing enables comprehensive transcriptome characterization but remains challenging to benchmark due to sequencing errors, sample variability, and the limited scope of existing evaluatio...

nature.com

And to close, the one who made it all possible. 🌅 Closing keynote: Prof. Ana Conesa on SQANTIverse, a unifying framework for long-read transcriptomics. LongTREC coordinator, VALT organiser, and a field-shaper for years. No one better to end on. Thank you, Ana. 💙 #VALT2026 #LongReads #SQANTI

VALT 2026 Round Table, in a few lines that stuck: 🔹 Move from the gene to the transcript as the unit of biology 🔹 Technical ground truth ≠ biology 🔹 A zoo of RNA modifications now waiting for AI to learn And of course: there'll be a next VALT. 🌅 #VALT2026 #LongReads

Where next? 🔮 Before the close, the Scientific Committee take on the VALT 2026 Round Table: The Future of Long-Read Transcriptomics. Three days of chromatin, benchmarking, proteoforms and RNA mods behind us, now the conversation turns to what comes next. #VALT2026 #LongReads

Roll up your sleeves: from long reads to proteins, hands-on. 🧬💻 This afternoon's workshop is Long-Read Proteogenomics with LRP2, led by Gloria Sheynkman, Megan Schertzer and Julia Lewandowski. #VALT2026 #LongReads #Proteogenomics

Aggressive cancers hide their complexity in the transcriptome. 🧬 Camilla Ugolini closes the session with BRIGHT, a long-read resource built to resolve transcript and epitranscriptomic complexity in aggressive breast cancer. #VALT2026 #LongReads #BreastCancer #Epitranscriptomics

RNA modifications that shift in real time. ⏱️ Logan Mulroney takes the stage to present direct RNA nanopore sequencing of human pancreatic beta cells, revealing how modifications change rapidly after glucose stimulation. #VALT2026 #LongReads #DirectRNA #Epitranscriptomics

Better quantification, not just more reads. 📊 Keynote: Prof. Kin Fai Au (Univ. of Michigan) on how long reads improve quantitative transcriptome analysis. Co-lead of the LRGASP benchmark and author of miniQuant, showing exactly where long reads earn their place. #VALT2026 #LongReads #Quantification

Thank you for a brilliant talk! Looking forward to what the @goekelab.bsky.social brings to the field of long read transcriptomics next!

Jonathan Göke@jonathangoeke.bsky.social · last mo.

Fantastic #longtrac meeting in Valencia, very happy to participate in the @nanoporetech.com workshop describing the recent updates in the cDNA protocol that doubles read length Postdoc positions available @goekelab.bsky.social to work on long read RNA seq (and Bambu)! github.com/GoekeLab

Day 3 opens with modifications. 🧬 First keynote: Dr Jana Jeschke (Institut Jules Bordet, ULB) on nanopore-resolved epitranscriptomic landscapes in human breast cancer. Reading RNA modifications directly in native molecules, mapped across real tumours. #VALT2026 #LongReads #Epitranscriptomics

Closing day 2. 🧬 Final keynote: Prof. Gloria Sheynkman (Univ. of Virginia) on going from long reads to proteoforms, linking transcript isoforms to the proteins they actually make. The perfect bookend to a day spent deep in isoforms. #VALT2026 #LongReads #Proteoforms

The genome is hiding more proteins than we've annotated. 🔬 Next: Nuo Xu on pairing long-read transcriptomics with Ribo-seq to expand the noncanonical proteome. Translation is happening well beyond the ORFs we already know about. #VALT2026 #LongReads #RiboSeq #Proteome

Long reads, full throttle. 🧬 This afternoon's workshop belongs to Pacific Biosciences, with Kinnex front and centre: large-scale automatable RNA-seq, full-length isoform workflows, and SQANTI tooling stretching from bulk to single-cell and single-nuclei. #VALT2026 #LongReads #Kinnex #IsoSeq

Flash Talks incoming. ⚡ 🫁 Colette Felton on novel driver alterations in lung cancer 🦎 Brian O'Toole on annotation across vertebrate orders 🧫 Mariana Ribeiro on FLIGHT-seq full-length isoform sequencing 🧠 Rosemary Bamford on isoform diversity in the human cortex

The ocean is full of genomes we've barely read. 🌊 Up next: LongTREC's Carmen Lafuente (Genoscope, CEA) revealing the hidden genetic diversity of marine plankton. Long reads opening up a corner of life that's been hard to see. #VALT2026 #LongReads #MarinePlankton #NonCannonicalSplicing

Heat, stress, and a genome with four copies of everything. 🥔 Next: our own Nadja Nolte (National Institute of Biology) on using long-read RNA-seq to study the heat stress response in polyploid potato. Long reads earning their keep in a properly tricky genome. #VALT2026 #LongReads #Polyploidy

Splicing patterns you can actually trust. 🔬 Next: Prof. Lauren McIntyre (Univ. of Florida) showing that variation in splice junctions is reproducible across technologies and conserved across species. Signal over noise- exactly what the field needs. #VALT2026 #LongReads #Splicing #Drosophila

Prof. Mark D. Robinson (Univ. of Zurich) on systematic benchmarking of long-read RNA-seq platforms and doing it properly with Omnibenchmark. Fair, reproducible comparisons from someone who's long pushed for them. #VALT2026 #LongReads

How do you annotate a lncRNA locus when the annotation simply isn't there yet? 🧬 Fabio Zanarello (CRG, Guigó lab) takes the stage next with his answer: transferring gene models across species to map lncRNA loci.

To join, or not to join? 🎭 That's the question Fabian Jetzinger is putting to us next, on handling biological replicates in lrRNA-seq data. #VALT2026 #LongReads #lrRNAseq #CallOrJoin

Up next! 🧬 We're delighted to hand over to LONGTREC's very own Yalan Bi (Max Planck Institute for Molecular Genetics): "Interactome predictions from long-read transcriptome sequencing (LRTS) data."

Day 2 is underway! ☀️ We're opening this morning with a keynote from Prof. Matthew Ritchie (WEHI): "Benchmarking and analysing long-read RNA-sequencing data with LongBench and FLAMES." Rigorous benchmarking and the tools to act on it. #VALT2026 #LongReads #Transcriptomics #LongTREC

Now at #VALT2026: the Oxford Nanopore workshop. Aino Järvelin kicks off, then Jonathan Göke on the cDNA beta test and library prep for long-read RNA-seq, and Christoph Dieterich on RNA modification co-occurrence from direct RNA-seq. #LongTReC #lrRNAseq

⚡ Flash talks at #VALT2026 ⚡: four quickfire takes on building and annotating transcriptomes with long reads: transcript-end detection in FLAIR4, the non-coding transcriptome, structural gene prediction, and tissue-specific isoform atlases. #LongTREC #lrRNAseq

Next at #VALT2026: Anastasiya Grinko on expanding the known transcriptome at the single-cell level, using long reads to surface novel isoforms in macrophages and monocytes from diseased tissues. #LongTREC #lrRNAseq #SingleCellLongRead

Next at #VALT2026: Juan Francisco Cervilla on evaluating gene fusion expression in B-ALL at single-cell resolution, using long reads to resolve fusion transcripts cell by cell. #LongTREC #lrRNAseq #FusionGenes

Next at #VALT2026: Maximillian Gabriel Marin on personalized transcriptome annotation, exposing reference-biased isoforms and how structural variation shapes human transcript diversity. #LongTREC #lrRNAseq

ext at #VALT2026: Fairlie Reese on long-read transcriptomics across a genetically diverse human cohort, revealing ancestry bias in gene annotation. Non-European transcripts are underrepresented in current references. #LongTREC #lrRNAseq