🧵 New pre-print out from my time at @mib-wur.bsky.social showing how the titration of the initiator protein DnaA on the chromosome of E. coli can generate stable DNA replication @hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social doi.org/10.64898/202...

🧵 Our study on the elusive titration control mechanism of the replication initiator DnaA in Escherichia coli is now out on @natcomms.nature.com‬! @stephkoe.bsky.social @hohlbeinlab.bsky.social‬ @nicoc-micsynmet.bsky.social @ettema.bsky.social‬ @mib-wur.bsky.social doi.org/10.1038/s414...

The Escherichia coli replication initiator DnaA is titrated on the chromosome - Nature Communications

A DNA-binding protein, DnaA, regulates the initiation of chromosomal replication in bacteria. Here, Olivi et al. show that the bacterial chromosome sequesters DnaA in a growth rate-dependent manner, s...

doi.org

Definitely an experience to say the least, but stoked to see it in its final form! Can't wait to share more

Johannes Hohlbein@hohlbeinlab.bsky.social · 12mo ago

Heroic effort by @loreoliv.bsky.social to wrap that one up! Reviewers' comments: similar number of words as in the manuscript... More soon.

I'm beyond excited to share that I've been awarded an @embo.org Postdoctoral Fellowship to join the lab of prof. Johan Elf at Uppsala University @uu.se! Can't wait to dive even deeper in the fascinating world of bacterial physiology!

This is the science that really matters

Giacomo Bartolucci (he)@gbart.bsky.social · 2y ago

1/n Some time ago my colleague, excellent cook, and friend Ivan told me: "Cacio e pepe is the recipe that I screw up more often. Let's make a project studying systematically the physics of that sauce". Prepare to get cheesy, I'm glad to share the Cacio e paper preprint: arxiv.org/abs/2501.00536

The tech bro fascination with eugenics is so on brand. The idea that you could make accurate, actionable predictions from individual genotypes (with all their complex, non-linear interactions) from averaged, linearly modeled population-level genomic statistics is just another big data fantasy

In the spirit of which, here some new filter wheels designed by Ian Kercher with more info available here: www.printables.com/model/110377... #OpenMicroscopy

Johannes Hohlbein@hohlbeinlab.bsky.social · 2y ago

Non-comprehensive list of projects, hardware, software, and people related to #OpenMicroscopy. List could do with some updates, so please share and suggest additions! github.com/HohlbeinLab/...

We strongly suggest that academic publishers and other platforms that host research rapidly implement a Share to Bluesky button for their articles. Here's how: docs.bsky.app/docs/advance... #AcademicSky #HigherEd #Altmetrics

Action Intent Links | Bluesky

Authors, websites, and apps can use action intent links to implement "Share on Bluesky" buttons, or similar in-app actions. Logged-in users will be directed to the corresponding action view in the Blu...

docs.bsky.app

The best resource for #microscopy is among us on BlueSky! Savior of my #sptPALM designs

FPbase@fpbase.org · 2y ago

Hello friends! We’re here to celebrate and share all things fluorescent. Tag us as you share findings related to the use or publication of FPs and other probes

Starting to think I should use our dog Ombra as a metaphor to explain students how fluorescent proteins allow you to tell apart things of interest from the background

I’m excited to share the first preprint from my postdoc at @johan_elf_ lab together with Konrad Gras. 🧬🦠 biorxiv.org/content/10.1... (1/14)

A dynamic 3D polymer model of the Escherichia coli chromosome driven by data from optical pooled screening

The DNA of bacterial cells is organized in a highly dynamic chromosome structure. To guarantee its propagation, the chromosome must replicate, segregate, and accommodate other biological processes lik...

biorxiv.org