Michi Forsthofer

@mforst.bsky.social

Postdoc in the Baden lab. Looking into plasticity of vision in frogs 🐸

1/10 New preprint on bioRxiv Nanodiamonds for spatial resolution benchmarking in two-photon microscopy We introduce nanodiamond-based phantoms as a robust, reusable alternative to bead–agarose samples for PSF calibration. www.biorxiv.org/content/10.6...

Nanodiamonds for spatial resolution benchmarking in two-photon microscopy

Reliable and reproducible measurement of spatial resolution is essential for validating and comparing the performance of two-photon microscopy systems. We present fluorescent nanodiamonds as robust, photostable, reusable, and biocompatible probes for benchmarking spatial resolution. Owing to their nanoscale dimensions and stable fluorescence, nanodiamonds act as near-ideal point-like emitters, enabling accurate characterisation of the point spread function across varying imaging conditions. We demonstrate that nanodiamond-based phantoms serve as a reliable alternative to conventional fluorescent beads embedded in agarose, while at the same time offering advantages in stability and optical properties. Our results position nanodiamond phantoms as a next-generation calibration material that bridges ease of use and reproducibility, advancing quantitative imaging and cross-platform comparability in modern fluorescence microscopy. ### Competing Interest Statement The authors have declared no competing interest. Wellcome Trust, https://ror.org/029chgv08, Investigator Award in Science 220277/Z20/Z European Research Council, https://ror.org/0472cxd90, ERC-StG “NeuroVisEco” 677687, ERC AdG “Cones4Action” covered under the UK’s EPSRC guarantee scheme EP/Z533981/1 UK Research and Innovation, https://ror.org/001aqnf71, BBSRC, BB/R014817/1, BBSRC, BB/W013509/1 International Human Frontier Science Program Organization, https://ror.org/02ebx7v45, RGP001/2025 Leverhulme Trust, https://ror.org/012mzw131, PLP-2017-005, RPG-2021-026, RPG-2-23-042 Lister Institute of Preventive Medicine Gdańsk University of Technology, https://ror.org/006x4sc24, Nobelium DEC-4/1/2024/IDUB/I.1a/No, Platinum 1/1/2025/IDUB/I.1B/Pt

biorxiv.org

Last night Adam Kampff, the glue, the light, the catalyst, the builder, the smile, left us. He and his work transformed the lives of many labs, scientists, students. He inspired and was generous to his last transformation, working tirelessly to set up a foundation to continue the work.Thank you!

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Excited for the European Retina Meeting next week, and to show some of my work on how 🐸 see the world as peacefully filter feeding tadpoles or predatory frogs. Here's a sneak peek at the imaging part, for more info talk to me or see my poster on the baden lab website: badenlab.org/posters/

👁️The retina — strikingly conserved across vertebrates, but an oddity among bilaterians! So how did it evolve? With @mikebok.bsky.social, @neurofishh.bsky.social and @denilsson.bsky.social, we argue that retinal complexity may 𝑝𝑟𝑒𝑑𝑎𝑡𝑒 𝑡ℎ𝑒 𝑒𝑦𝑒 𝑖𝑡𝑠𝑒𝑙𝑓. www.biorxiv.org/content/10.1... 1/n

a black and white dog is sitting on a couch with its tongue sticking out .

ALT: a black and white dog is sitting on a couch with its tongue sticking out .

media.tenor.com

New paper on bioRxiv: "Ambient Light Impacts Innate Behaviors of New-World and Old-World Mice" - together with @farrowlab.bsky.social, with data from NERF master students and two of my lab members! A study across 3 species: Mus musculus, Peromyscus maniculatus & P. polionotus doi.org/10.1101/2025...

Ambient Light Impacts Innate Behaviors of New-World and Old-World Mice

Animals encounter predators and prey under diverse lighting conditions that signal different risks and opportunities, yet how ambient illumination shapes innate approach and avoidance behaviors remain...

doi.org