1/5 How much phosphorylation can a single kinase deposit? In our new study with @typaslab.bsky.social out in @nature.com, phage T7's kinase (T7K) modifies almost all the E. coli proteome + more densely than all ~500 kinases do our own proteomes 🤯 www.nature.com/articles/s41...

Pervasive phosphorylation by phage T7 kinase disarms bacterial defences - Nature

The T7 bacteriophage deactivates bacterial defence systems through T7K phosphorylation of nearly all host and phage proteins during infection.

nature.com

Our new paper „Variations in the latitudinal diversity gradients of the ocean microbiome“ was just published in @cp-cellhostmicrobe.bsky.social 🥳 We present a global analysis of prokaryotic #biodiversity in the surface and mesopelagic layers of the ocean 🦠🌊 www.cell.com/cell-host-mi...

Variations in the latitudinal diversity gradients of the ocean microbiome

Eriksson et al. conduct a global marine microbiome diversity analysis and demonstrate how the surface ocean latitudinal diversity gradient is disproportionately shaped by a few species-rich taxa. Mask...

cell.com

One of the main stories of my postdoc is finally out! It builds on an intuition from my early days in omics data analysis: our signaling pathways are simply too small, underrepresenting cellular complexity after stimulation. Something obvious, yet surprisingly hard to quantify.

Saez-Rodriguez Group@saezlab.bsky.social · 7mo ago

Interested in kinase-driven signaling interactions? Check out our (now peer-reviewed) paper together with @savitski-lab.bsky.social on reconstructing signaling networks from phosphoproteomics data and prior knowledge: ➡️ doi.org/10.1038/s414...

Unbelievable, but I am part of this lab for half of its life 👵 It just keeps getting better ☺️

Savitski Lab@savitski-lab.bsky.social · 7mo ago

10 years and still standing strong @EMBL 🍾💪 Huge thanks to all members, alumni, supporters & colleagues who made it possible. Here’s to the next decade! 🙏

Happy to share new work led by @miraburtscher.bsky.social, together with colleagues from @savitski-lab.bsky.social, @saezlab.bsky.social (shout-out to @martingarridorc.bsky.social), @zimmermannlab.bsky.social and others. Original post + paper below! 👇

Savitski Lab@savitski-lab.bsky.social · 7mo ago

Rewiring of oncogenic signaling in #DrugResistance is a moving target. In our new study, we used biophysical phosphoproteomics to investigate #BRAF mutant cancer, linking phosphorylation changes to protein function and #MolecularMechanisms through #Multi-Omics integration. tinyurl.com/funsignaling

When I first learned about omics at university, I confidently stopped caring about single proteins. Fast-forward 4 years of a system’s biology PhD, I now have the most amazing favorite single protein which I can’t stop thinking about. Here is the story of how that happened 👀

Savitski Lab@savitski-lab.bsky.social · 7mo ago

Rewiring of oncogenic signaling in #DrugResistance is a moving target. In our new study, we used biophysical phosphoproteomics to investigate #BRAF mutant cancer, linking phosphorylation changes to protein function and #MolecularMechanisms through #Multi-Omics integration. tinyurl.com/funsignaling

Never thought I'd co-first author a paper that appears on the screens on campus, that makes me very happy😃 www.cell.com/cell/fulltex... Check out the paper and get amazing summaries from the EMBL communications team and a thread by @chanyeong-kim.bsky.social

EMBL@embl.org · 7mo ago

In the largest study of its kind, scientists in the Bork Group at EMBL have found that a small subset of microbes can carry and transfer genes across disparate habitats, creating a planet-wide, interconnected network of microbiomes 🌍 🦠 🔗 Read more here: www.embl.org/news/science...

Just published: "Planetary microbiome structure and generalist-driven gene flow across disparate habitats" by @podlesny.bsky.social @chanyeong-kim.bsky.social and @jonas-bio.bsky.social www.cell.com/cell/fulltex... See the quoted post below for a thread on the preprint!

Planetary microbiome structure and generalist-driven gene flow across disparate habitats

A planetary-scale analysis of over 85,000 metagenomes establishes a framework for exploring the structure and drivers of global microbial habitats, revealing that generalist species bridge ecological ...

cell.com

Chan Yeong Kim@chanyeong-kim.bsky.social · last yr.

Our new preprint is out! www.biorxiv.org/content/10.1... In this study, we present the largest systematic analysis of microbiome structure and function, integrating 85K uniformly processed metagenomes from diverse habitats worldwide. @podlesny.bsky.social @jonas-bio.bsky.social @borklab.bsky.social

New year, new preprint! 🎊 We are excited to share our recent work on #E3 ligase regulation in #metabolism! www.biorxiv.org/content/10.6... #ubiquitin #targetedproteindegradation #chemicalbiology 1/6

Want to learn about the theoretical aspects of proteins/peptides analysis by mass spectrometry? Then join the #EMBLProteomics course where you will get hands-on experience in sample preparation and perform tandem mass tag labelling. Apply by 3 March:https://s.embl.org/sdp26-01-bl 🔬💻

Finally out! 🤩 Check out our HT- PELSA for high throughput screening！

Savitski Lab@savitski-lab.bsky.social · 11mo ago

Happy to see our HT-PELSA paper now published in @natsmb.nature.com 🎊 Big thanks for the constructive review process! 📖Read the manuscript here (www.nature.com/articles/s41...) & check the thread for additional information ⬇️

Happy to see our HT-PELSA paper now published in @natsmb.nature.com 🎊 Big thanks for the constructive review process! 📖Read the manuscript here (www.nature.com/articles/s41...) & check the thread for additional information ⬇️

High-throughput peptide-centric local stability assay extends protein–ligand identification to membrane proteins, tissues and bacteria - Nature Structural & Molecular Biology

Li et al. further develop a high-throughput peptide-centric local stability assay that speeds up sample preparation 100-fold and extends protein–ligand identification to membrane proteins, tissues and...

nature.com

Savitski Lab@savitski-lab.bsky.social · last yr.

Want to know how the ligands interact with proteins beyond model cell lines, e.g., in tissues or bacteria? Interested in membrane targets? Check out our High-Throughput PELSA method which allows you do all these cool screenings for dozens of ligands within two hours! www.biorxiv.org/content/10.1...

How can we find out what’s really going on inside cells when we’re generating so much complex data? CORNETO is an open-source tool that uses machine learning to turn tangled omics datasets into clear maps of how genes, proteins, and signalling pathways interact. www.ebi.ac.uk/about/news/r... 🧪

CORNETO: machine learning to decode complex omics data

New tool combines biological knowledge with machine learning to help researchers extract meaningful insights from complex omics data.

ebi.ac.uk

integrated temporally resolved transcriptomic, proteomic, and secretomic profiling of human kidney mesenchymal cells from @savitski-lab.bsky.social @saezlab.bsky.social @miraburtscher.bsky.social identify dynamic changes upon TGF-beta stimulation ➡️ www.embopress.org/doi/full/10....

Big congrats to everyone involved - it was a blast! 🎊 If you're interested in #multiomics integration or #kidneyfibrosis, check it out! ⬇️

Saez-Rodriguez Group@saezlab.bsky.social · last yr.

The final version of our multi-omics study on kidney fibrosis is out now (tinyurl.com/kidneyfibMSB). Together w/ Pepperkok + Savitski labs @embl.org, we present a time-resolved #multiomics + computational network modeling approach in combination w/ phenotypic assays to study #kidneyfibrosis

Ever wondered which method to use to infer kinase activities from phosphoproteomics data? 💻💭Our revised comprehensive evaluation of kinase activity inference tools, done in collaboration with the Zhang lab @bcmhouston.bsky.social, is now out @natcomms.nature.com 🔬 tinyurl.com/4twuc6z4

Online now - the Spotlight "Accelerating the stride toward functional #glycoproteomics" from @timveth.bsky.social and @nmriley.bsky.social. #Glycoproteins #Glycoforms #Glycoprofiling #DQGlyco #Glycotime authors.elsevier.com/a/1l3k53S6Gf...

Want to know how the ligands interact with proteins beyond model cell lines, e.g., in tissues or bacteria? Interested in membrane targets? Check out our High-Throughput PELSA method which allows you do all these cool screenings for dozens of ligands within two hours! www.biorxiv.org/content/10.1...

High-throughput peptide-centric local stability assay extends protein-ligand identification to membrane proteins, tissues, and bacteria

Systematic mapping of protein-ligand interactions is essential for understanding biological processes and drug mechanisms. Peptide-centric local stability assay (PELSA) is a powerful tool for detectin...

biorxiv.org

Excited to share our new paper! If you’re interested in glycoproteomics, be sure to check it out 👇 Huge kudos to all coauthors, especially the amazing glycoteam: Clement, @miraburtscher.bsky.social, Isabelle, and Misha (@savitski-lab.bsky.social)!

Savitski Lab@savitski-lab.bsky.social · 2y ago

We are very happy to present our work on N-glycoproteomics!🍬 Our method enables the selective enrichment and precise quantification of intact N-glycopeptides to explore the dynamics of glycosylation microheterogeneity. www.nature.com/articles/s41...

Bacteria can influence how sugars modify proteins in the brain – shown for the first time by EMBL researchers. In their study, the scientists describe a new method to study glycosylation systematically & quantitatively, leading to new biological insights. 🧪🧠📈 www.embl.org/news/science...

Excited to see this huge piece of work out 🎉 Congrats to everyone involved! If you ever wanted to read a 8in1 paper grab a cup of tea and enjoy 📖

Savitski Lab@savitski-lab.bsky.social · 2y ago

We are very happy to present our work on N-glycoproteomics!🍬 Our method enables the selective enrichment and precise quantification of intact N-glycopeptides to explore the dynamics of glycosylation microheterogeneity. www.nature.com/articles/s41...