Niko Klink
@nikolasklink.bsky.social
Chemical Biology & drug discovery @Max Planck Dortmund | All things Ubiquitin and bifunctionals @Gersch Lab.
Very happy to see our contribution to a better understanding of Hsp70/90 #chaperones online! Congrats and a big thank you to all co-authors, collaborators and reviewers! #proteostasis @poepsel-lab.bsky.social @crc1430.bsky.social @unidue-zmb.bsky.social
Nature Structural & Molecular Biology@natsmb.nature.com · last yr.New online: Conformational plasticity of a BiP–GRP94 chaperone complex
Really excited to share the first chapter of my PhD as a preprint titled: Targeted degradation of USP7 in solid cancer cells reveals disparate effects of deubiquitinase inhibition vs. acute protein depletion: www.biorxiv.org/content/10.1.... If that sounds interesting to you, small tweetorial below👇
Targeted degradation of USP7 in solid cancer cells reveals disparate effects of deubiquitinase inhibition vs. acute protein depletion
Proteolysis-targeting chimeras (PROTACs) co-op the ubiquitin system for targeted protein degradation, creating opportunities to interrogate cellular functions of proteins through "chemical knockdown"....
biorxiv.org
Very excited to share that our work on Parkinson's target USP30 has been published in Nature Structural & Molecular Biology! By combining soluble DUB-domains onto USP30, we for the first time achieved co-crystallization with a small molecule (NK036) without Nanobodies! www.nature.com/articles/s41...
Chimeric deubiquitinase engineering reveals structural basis for specific inhibition of the mitophagy regulator USP30 - Nature Structural & Molecular Biology
Kazi et al. report the crystal structure of the mitochondrial deubiquitinase USP, a clinical stage Parkinson’s disease drug target, in complex with a specific inhibitor. The authors delineate a framew...
nature.com
Wow. This is really stunning . www.science.org/doi/10.1126/...
In-cell architecture of the mitochondrial respiratory chain
Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, workin...
science.org
I'm super excited to share our study in collaboration with @jnpruneda.bsky.social, published today in @embojournal.org! We show that PARPs are modified with a non-canonical ester-linked mono-ADP-ribosylated ubiquitin species for the first time in cells: www.embopress.org/doi/full/10....
Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose | The EMBO Journal
imageimageCertain E3 ligases have been found to ubiquitylate hydroxyl groups on free NAD+ and ADP-ribose in vitro, but the in vivo occurrence of this dual post-translational modification has remained ...
embopress.org
🎉Super excited to share our story on how the substrate receptor FBXO31 functions as a quality control factor by recognizing amides. This has been an amazing collaboration between Bode lab and @jcornlab.bsky.social. Special shutout goes to @matthiasmuhar.bsky.social www.nature.com/articles/s41...
C-terminal amides mark proteins for degradation via SCF–FBXO31 - Nature
SCF–FBXO31 scans proteins for C-terminal amidation and marks them for subsequent proteasomal degradation.
nature.com
A fantastic story from our lab about finding novel activity in two "inactive" DUBs!
Malte Gersch@maltegersch.bsky.social · 2y agoCould't think of a better inaugural post here. Very excited to share that our manuscript on the "Discovery and mechanism of K63-linkage-directed deubiquitinase activity in USP53" was published today at Nature Chemical Biology. Congratulations Kim, Kai, and all authors! www.nature.com/articles/s41...
