Nicolas Chiaruttini

@nkiaru.bsky.social

Bio-Image Analyst for the Biochemistry Department at UNIGE https://nicolas.chiaruttini.org

Absolutely butchering my manuscript in order to appease my reviewers. It's like playing jenga, but in addition to removing vital structural integrity, you add completely random shit like a cactus here, a dildo there, some hippo turd on the side, and sprinkle it all with dead bees. ta-da, science.

Claude code flexing: ---- For your case with uniform TIFFs on network storage, lazy validation would transform a multi-hour init into seconds. The validation cost is amortized across actual reads. ---- Let's see if that's true!

We are happy to share our latest work, 4Pi-SIMFLUX, which combines structured illumination with interferometric detection to achieve near-isotropic 3D localization precision of 2–3 nm and resolve sub-10 nm structural features across whole mammalian cells. www.nature.com/articles/s41... rdcu.be/eQVxt

4Pi-SIMFLUX: 4Pi single-molecule localization microscopy with structured illumination - Nature Methods

4Pi-SIMFLUX is a single-molecule localization microscopy approach that achieves a near-isotropic resolution below 10 nm in whole mammalian cells.

nature.com