Tessera

@rioscolombo.bsky.social

Nature Protocols @natprot.nature.com · 5mo ago

New Article! Establishing and analyzing the Simplified Human Intestinal Microbiota (SIHUMI) as a versatile in vitro gut microbiome model with qPCR-based strain-level tracking

Establishing and analyzing the Simplified Human Intestinal Microbiota (SIHUMI) as a versatile in vitro gut microbiome model with qPCR-based strain-level tracking

Nature Protocols, Published online: 19 March 2026; doi:10.1038/s41596-026-01336-yThis protocol enables users to establish a defined, in vitro consortium composed of seven fully sequenced and anaerobically culturable human gut bacteria and follow the growth of individual members via strain-specific qPCR to evaluate community dynamics in response to interventions.

dlvr.it

@rioscolombo.bsky.social · 5mo ago

I’m excited to share our latest publication in @natureportfolio.nature.com for researchers to set up their own in vitro gut microbiome model with strain-level resolution. Free access to the article here: rdcu.be/e81bY

Establishing and analyzing the Simplified Human Intestinal Microbiota (SIHUMI) as a versatile in vitro gut microbiome model with qPCR-based strain-level tracking

Nature Protocols - This protocol enables users to establish a defined, in vitro consortium composed of seven fully sequenced and anaerobically culturable human gut bacteria and follow the growth of...

rdcu.be

APC Microbiome Ireland@apcmicrobiomeirel.bsky.social · 5mo ago

New in Nature Protocols buff.ly/DUCGVAf A standardised method for reproducible tracking of gut bacteria via the SIHUMI microbial community. Congrats to APC researchers Natalia Ríos Colombo, Mariana Perez-Ibarreche, P.Ross & C.Hill, in collab with Pranav Lanka at @tyndallinstitute.bsky.social

APC Microbiome Ireland @apcmicrobiomeirel.bsky.social · 6mo ago

New paper from the CEB- @uminho.bsky.social & APC 𝐄𝐯𝐚𝐥𝐮𝐚𝐭𝐢𝐨𝐧 𝐨𝐟 𝐭𝐡𝐞 𝐝𝐞𝐥𝐢𝐯𝐞𝐫𝐲 𝐨𝐟 𝐚𝐧 𝐚𝐧𝐭𝐢-𝐋𝐢𝐬𝐭𝐞𝐫𝐢𝐚 𝐞𝐧𝐝𝐨𝐥𝐲𝐬𝐢𝐧 𝐯𝐢𝐚 𝐂𝐑𝐈𝐒𝐏𝐑-𝐂𝐚𝐬𝟗 𝐞𝐧𝐠𝐢𝐧𝐞𝐞𝐫𝐞𝐝 𝐩𝐫𝐨𝐛𝐢𝐨𝐭𝐢𝐜 𝑺𝒂𝒄𝒄𝒉𝒂𝒓𝒐𝒎𝒚𝒄𝒆𝒔 𝒃𝒐𝒖𝒍𝒂𝒓𝒅𝒊𝒊 Appl Microbiol Biotechnol buff.ly/pDDfR3f #CRISPRCas9 @ellen-murr.bsky.social @rioscolombo.bsky.social @colinhillucc.bsky.social

We previously engineered Saccharomyces cerevisiae through CRISPR-Cas9 integration to produce Ply511, a bacteriophage endolysin active against L. monocytogenes, showing the potential of engineered yeast to produce endolysins for biocontrol. In this study, we extended this approach to the probiotic yeast S. boulardii and directly compared the two yeasts as secretion hosts for Ply511. Using a simulated human gastrointestinal environment, we evaluated their ability to retain endolysin activity and reduce L. monocytogenes levels.