To sequence a cell, you've always had to destroy it. POND-seq changes that. Secretory nanocages export RNA complexes into supernatant — cells stay alive, beacons of RNA keep broadcasting. Science fiction turned methodology. #RNA #RNASky #RNAseq #RBP #RNAbiology #Transcriptomics

Molecular Cell @cp-molcell.bsky.social · 2w ago

Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages

Publicly available resource: 93K+ chemical perturbations meets #transcriptomic profiling. Rare transcript patterns reveal cell states — including ferroptosis resistance — that smaller-scale approaches miss entirely. This is where the field is going. #RNASky #RNA #RNAseq #GeneExpression

Nature Methods@natmethods.nature.com · 12mo ago

Chemical-induced gene signatures (CIGS) is a high-resolution dataset of 319,045,108 gene expression events induced by chemical perturbation. www.nature.com/articles/s41...

Nature Biomed Eng (July 7): non-capped, non-polyadenylated RNA oligonucleotides efficiently activate CD8+ T cells in vivo. No canonical mRNA features required for translation or immunogenicity. The field's model of how RNA is translated just got more complicated. #RNASky #RNA #ChemRNA

Nature Biomedical Engineering@natbiomedeng.nature.com · 4w ago

Chemically synthesized, non-capped and non-polyadenylated peptide-coding RNA efficiently induces antigen-specific CD8+ T cells

RNA hydrolysis at elevated RT temps isn't a footnote. It's actively degrading your long transcripts during the reaction. New data at #RNATx '26 quantifies how much and shows what changes with an RT at 30°C. 3 modalities: SR, PacBio LR, and ONT DRS. The gains for transcripts ≥10kb are substantial.

uMRT® has peer-reviewed publications across 5 application areas on our website. Find the work most relevant to your research, from rare #transcriptprofiling to #RNAmods mapping, #shortread or #longread #RNAseq; access key research within one click! See🔗below! #lncRNA #UltraMarathonRT #RNASky

Dr. Graveley's lab used uMRT to tackle the most complex #alternativesplicing in any model organism, #Drosophila #DSCAM, with its thousands of possible isoforms, and #circRNAs accumulating with age. His talk is a masterclass in what LR #RNASeq can do when your RT doesn't give up. bit.ly/4tNiZ1T

Long transcripts still get lost in #ONT direct RNA-seq without the right RT. Tonight at #RNA2026, we're sharing new data on how uMRT fixes that. (P2-293) Improved long transcript representation in ONT direct RNA-seq with UltraMarathonRT® — tonight, 8:30–10:30 PM, Room 517bc & 520 #RNA #RNASeq

MMLVs stall on long, structured RNA, and the mischaracterizations of the transcriptome often go undetected in your data. We're presenting new data at #RNA2026 tonight! May 27, 8–10 PM (ID# 672) Ultra long transcript identification in long-read RNA-seq with uMRT 🔗 👇 @rnasociety.bsky.social #RNA

If you're running #ONT Direct RNA Seq with the current Induro protocol, your #RNA may be partially degraded before it ever reaches the pore. A new preprint demonstrates that substantial RNA hydrolysis occurs with RT buffer conditions at 60°C and that uMRT at 30°C is a better solution. 🔗👇 #RNASky

Detained introns. Alternative polyadenylation. Complex isoforms. Dr. Paul Boutz (U Rochester) showed at our recent webinar that LR-seq with uMRT captures what short-read methods miss — including in cancer transcriptomics. Watch now: bit.ly/4tNiZ1T #RNA #RNASky #ONT #nanoporeconf #RNASeq

Your RNA-seq library prep may be hiding biology from you. We'll be at #RNA26 in Montréal showing how UltraMarathonRT reveals RNA biology that retroviral RTs miss. Come find us in the Exhibitor Hall or at our poster sessions. Details in the link below 👇 #RNASeq #RNA #RNASky

Get more from the pore! RNAConnect Sr Sci and LR #RNASeq fanboy George Maio, PhD, gave a sneak preview of our newest #ONT #DRS and #cDNA data at the JAXLRSW earlier this week. Bottom line: More of those long isoforms you’ve been missing are now in reach. #RNA #RNASeq #Nanoporeconf #uMRT

#ONT #DRS is great for long #RNAs, unless they’re hydrolyzed during library prep. During this webinar, Dr. Underwood showed how uMRT outperforms other RTs during library prep. Full-length cDNA synthesis = more reads w/longer transcripts (shown here with human brain) bit.ly/4tNiZ1T #Nanoporeconf

At #JAXLRSW26 this week? Catch us tomorrow, May 12: ▪ Talk @ 3:15 PM: Long transcript representation in #ONT #DRS with uMRT ▪ Posters @ 4:00–5:45 PM If you’re chasing isoforms, lncRNAs, or 3’ UTR structure, come talk to us directly or ✉️ info@rnaconnect.com. #RNASky #RNASeq @jax.org

Heading to the JAX Long Read Sequencing Workshop, May 11–14? Connect with RNAConnect to discuss long-read RNA library prep, isoform detection, and getting more from your #PacBio or #ONT data. @jax.org #JAXLRSW26 #RNASeq

Attending the 2026 NCI RNA Workshop at the #NIH tomorrow? Don't miss our poster #21, "Improved Data Quality in Short Read and Long Read RNA-seq Using an Ultraprocessive Reverse Transcriptase That Copies at 30°C." We'd love to learn more about your research and see if we have tools that can help.

Turn your toughest RNA-Seq challenges into publishable data with uMRT, which provides 10–30% more genes detected & truly uniform gene body coverage. Visit us at Booth #302 at #ABRF26, March 28–31 in Pittsburgh, PA! Learn more at the 🔗 in the comments. #RNASeq @abrf.bsky.social

Missing isoforms? Uneven RNA-Seq coverage? Jason Underwood has answers today! @jg-underwood.bsky.social 📍 Poster 476 📅 Wed, Feb 25 | 4:45–6:10 p.m. New methods for more accurate, complete RNA-Seq data. Don't miss it at #AGBT26.

RNAConnect@rnaconnect.bsky.social · 6mo ago

🎶 Where at #AGBT26 is…Jason Underwood? Find Jason. He’s the one introducing new methods for getting more accurate and complete #RNASeq data at Poster 476 on Feb 25, 4:45–6:10 p.m. Catch him → Schedule a partnership chat to explore integrations and joint studies. 👀🔗⬇️

Join us tomorrow (Feb 19) for a live discussion on overcoming key limitations in conventional #RNASeq, including incomplete transcript capture and structural bias, and exploring newer approaches that improve data resolution and confidence. Register via the link in the comments. #RNA #RNASky

RNAConnect@rnaconnect.bsky.social · 6mo ago

Methods and tools for RNA-seq are advancing rapidly. Are you aware of the drawbacks of historical methods and how you can improve resolution, reduce bias, and elevate confidence in your results? Learn more as our speakers share data and discuss the latest methods for #RNAseq. bit.ly/4rm6adZ

🎶 Where at #AGBT26 is…Jason Underwood? Find Jason. He’s the one introducing new methods for getting more accurate and complete #RNASeq data at Poster 476 on Feb 25, 4:45–6:10 p.m. Catch him → Schedule a partnership chat to explore integrations and joint studies. 👀🔗⬇️

Thanks to the following sponsors who are supporting the 2026 JAX Long-Read Sequencing Workshop! @sagescience.bsky.social @twistbioscience.com @pacbio.bsky.social @nebiolabs.bsky.social @idtdna.bsky.social @rnaconnect.bsky.social @nabsys.bsky.social @iconpcr.bsky.social Illumina Sampled

Methods and tools for RNA-seq are advancing rapidly. Are you aware of the drawbacks of historical methods and how you can improve resolution, reduce bias, and elevate confidence in your results? Learn more as our speakers share data and discuss the latest methods for #RNAseq. bit.ly/4rm6adZ

Now Available! The UltraMarathonRT® Direct RNA‑Seq Kit is compatible with your existing ONT protocol, designed to generate higher cDNA yields and improved performance on long/structured RNAs. Order now: bit.ly/4ju71Xl #RNA #RNAseq #NGS #nanopore #LongRead #RNASky

Ditch the doomscrolling and read the latest data showing how uMRT can improve your ONT direct RNA-Seq. Highlights: • Notable gains for >5 kb • ↑ NIC & NNIC + ↑ intron retention • +~25% higher cDNA yield Dive into the data → www.biorxiv.org/content/10.6... #RNA #RNAseq #DRS #cDNA #RNASky

The UltraMarathonRT® Direct RNA‑Seq Kit preserves and detects longer #RNAs and reveals more isoforms per gene. This kit offers a simple drop‑in fit to your #Nanopore SQK‑RNA004 workflow. See how it expands what you can learn from every #longread seq sample. PR🔗below. #RNASeq #DRS

What if you could see more transcripts from each of your #RNA samples? uMRT has been engineered to efficiently copy even the most difficult RNA targets, including: 🔹 Highly structured RNAs 🔹 Long transcripts (≤20kb) 🔹 High GC content regions 🔹 Non-coding RNAs Learn more: bit.ly/488ScFO

Reveal What Retroviral RTs Miss. Unlock deeper RNA-seq insights with uMRT, including: 🔹 Low-abundance transcripts 🔹 High GC content regions 🔹 Unique isoforms 🔹 Non-coding RNAs ...and more Enhance your RNA-seq today! Read more in the comments below... #RNASeq #RNA #GeneDetection #BioTech

uMRT has been engineered to deliver ultra-processivity at 30°C. It copies every RNA end-to-end in a single pass, which reveals more genes, transcripts, isoforms, and even twice as many retained introns as leading retroviral RT kits. Learn more: bit.ly/488ScFO #GeneDetection #RNASeq

[1/2] A new publication from Warkentin & Pyle presents a practical workflow for accurate circRNA detection using UltraMarathonRT® (uMRT), a highly processive reverse transcriptase. Pub 🔗 in the comments. #cDNA #RNA #RNAseq #circRNA #RTPCR #RNASky

We’re excited to (RNA)Connect with you at #ASHG25! Visit Booth #121 to discover how uMRT reveals more from every RNA sample. ▪️ 10–30% more genes detected ▪️ Superior quantification of long transcripts See what your #RNASeq data has been missing. #RNA #cDNA #RNASky #transcriptomics #genomics