RNAConnect
@rnaconnect.bsky.social
Ultra-performance enzymes to power a new multiomic era 📍Branford, CT 🔗www.rnaconnect.com
Congratulations to Leslie Watkins, Alan Zhu & senior author Bin Wu at Johns Hopkins on identifying a pervasive #RTqPCR artifact that inflates apparent knockdown across RNA-targeting #CRISPR systems (Csm, PspCas13b, CasRx). Rigorous work the CRISPR field needed. 🎉
Nature Biotechnology@natbiotech.nature.com · last wk.A pervasive RT–qPCR artifact inflates RNA knockdown by RNA-targeting CRISPR - @binwu1.bsky.social @hopkinsmedicine.bsky.social www.nature.com/articles/s41...
We couldn't be more excited for the start of the 2026 UCSC RNA futures meeting. It's going to be a fun week!!#RNAFutures2026. share.google/4CYqqExEx69i...
We are exhibiting at #RNAFutures this week! Stop by our booth, and Jason Underwood will talk through how to improve your RNA-seq by getting more genes and longer, more complete transcripts with #uMRT. #RNA #RNAseq #DRS #transcriptomics @jg-underwood.bsky.social
Steady-state transcriptomics hides the synthesis and decay kinetics that shape gene expression. AIR-seq, a new method from Harvard's Churchman lab, marks newly synthesized #RNA directly in standard #RNAseq libraries, no chemical conversion or enrichment required. #RNASky #bulkRNA #scRNA 🗞️🔗⬇️
bioRxiv Genomics@biorxiv-genomic.bsky.social · last mo.Analog intrinsic recoding measures RNA dynamics without chemical conversion https://www.biorxiv.org/content/10.64898/2026.07.27.741041v1
To sequence a cell, you've always had to destroy it. POND-seq changes that. Secretory nanocages export RNA complexes into supernatant — cells stay alive, beacons of RNA keep broadcasting. Science fiction turned methodology. #RNA #RNASky #RNAseq #RBP #RNAbiology #Transcriptomics
Molecular Cell@cp-molcell.bsky.social · 2mo agoLongitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages
Publicly available resource: 93K+ chemical perturbations meets #transcriptomic profiling. Rare transcript patterns reveal cell states — including ferroptosis resistance — that smaller-scale approaches miss entirely. This is where the field is going. #RNASky #RNA #RNAseq #GeneExpression
Nature Methods@natmethods.nature.com · last yr.Chemical-induced gene signatures (CIGS) is a high-resolution dataset of 319,045,108 gene expression events induced by chemical perturbation. www.nature.com/articles/s41...
Nature Biomed Eng (July 7): non-capped, non-polyadenylated RNA oligonucleotides efficiently activate CD8+ T cells in vivo. No canonical mRNA features required for translation or immunogenicity. The field's model of how RNA is translated just got more complicated. #RNASky #RNA #ChemRNA
Nature Biomedical Engineering@natbiomedeng.nature.com · 2mo agoChemically synthesized, non-capped and non-polyadenylated peptide-coding RNA efficiently induces antigen-specific CD8+ T cells
RNA hydrolysis at elevated RT temps isn't a footnote. It's actively degrading your long transcripts during the reaction. New data at #RNATx '26 quantifies how much and shows what changes with an RT at 30°C. 3 modalities: SR, PacBio LR, and ONT DRS. The gains for transcripts ≥10kb are substantial.
uMRT® has peer-reviewed publications across 5 application areas on our website. Find the work most relevant to your research, from rare #transcriptprofiling to #RNAmods mapping, #shortread or #longread #RNAseq; access key research within one click! See🔗below! #lncRNA #UltraMarathonRT #RNASky
Dr. Graveley's lab used uMRT to tackle the most complex #alternativesplicing in any model organism, #Drosophila #DSCAM, with its thousands of possible isoforms, and #circRNAs accumulating with age. His talk is a masterclass in what LR #RNASeq can do when your RT doesn't give up. bit.ly/4tNiZ1T
MMLVs stall on long, structured RNA, and the mischaracterizations of the transcriptome often go undetected in your data. We're presenting new data at #RNA2026 tonight! May 27, 8–10 PM (ID# 672) Ultra long transcript identification in long-read RNA-seq with uMRT 🔗 👇 @rnasociety.bsky.social #RNA
Detained introns. Alternative polyadenylation. Complex isoforms. Dr. Paul Boutz (U Rochester) showed at our recent webinar that LR-seq with uMRT captures what short-read methods miss — including in cancer transcriptomics. Watch now: bit.ly/4tNiZ1T #RNA #RNASky #ONT #nanoporeconf #RNASeq
#ONT #DRS is great for long #RNAs, unless they’re hydrolyzed during library prep. During this webinar, Dr. Underwood showed how uMRT outperforms other RTs during library prep. Full-length cDNA synthesis = more reads w/longer transcripts (shown here with human brain) bit.ly/4tNiZ1T #Nanoporeconf
Heading to the JAX Long Read Sequencing Workshop, May 11–14? Connect with RNAConnect to discuss long-read RNA library prep, isoform detection, and getting more from your #PacBio or #ONT data. @jax.org #JAXLRSW26 #RNASeq
Attending the 2026 NCI RNA Workshop at the #NIH tomorrow? Don't miss our poster #21, "Improved Data Quality in Short Read and Long Read RNA-seq Using an Ultraprocessive Reverse Transcriptase That Copies at 30°C." We'd love to learn more about your research and see if we have tools that can help.
Turn your toughest RNA-Seq challenges into publishable data with uMRT, which provides 10–30% more genes detected & truly uniform gene body coverage. Visit us at Booth #302 at #ABRF26, March 28–31 in Pittsburgh, PA! Learn more at the 🔗 in the comments. #RNASeq @abrf.bsky.social
Missing isoforms? Uneven RNA-Seq coverage? Jason Underwood has answers today! @jg-underwood.bsky.social 📍 Poster 476 📅 Wed, Feb 25 | 4:45–6:10 p.m. New methods for more accurate, complete RNA-Seq data. Don't miss it at #AGBT26.
RNAConnect@rnaconnect.bsky.social · 7mo ago🎶 Where at #AGBT26 is…Jason Underwood? Find Jason. He’s the one introducing new methods for getting more accurate and complete #RNASeq data at Poster 476 on Feb 25, 4:45–6:10 p.m. Catch him → Schedule a partnership chat to explore integrations and joint studies. 👀🔗⬇️
Join us tomorrow (Feb 19) for a live discussion on overcoming key limitations in conventional #RNASeq, including incomplete transcript capture and structural bias, and exploring newer approaches that improve data resolution and confidence. Register via the link in the comments. #RNA #RNASky
RNAConnect@rnaconnect.bsky.social · 7mo agoMethods and tools for RNA-seq are advancing rapidly. Are you aware of the drawbacks of historical methods and how you can improve resolution, reduce bias, and elevate confidence in your results? Learn more as our speakers share data and discuss the latest methods for #RNAseq. bit.ly/4rm6adZ
Thanks to the following sponsors who are supporting the 2026 JAX Long-Read Sequencing Workshop! @sagescience.bsky.social @twistbioscience.com @pacbio.bsky.social @nebiolabs.bsky.social @idtdna.bsky.social @rnaconnect.bsky.social @nabsys.bsky.social @iconpcr.bsky.social Illumina Sampled
Methods and tools for RNA-seq are advancing rapidly. Are you aware of the drawbacks of historical methods and how you can improve resolution, reduce bias, and elevate confidence in your results? Learn more as our speakers share data and discuss the latest methods for #RNAseq. bit.ly/4rm6adZ
What if you could see more transcripts from each of your #RNA samples? uMRT has been engineered to efficiently copy even the most difficult RNA targets, including: 🔹 Highly structured RNAs 🔹 Long transcripts (≤20kb) 🔹 High GC content regions 🔹 Non-coding RNAs Learn more: bit.ly/488ScFO
