Core2Edge: a human glioblastoma organoid–brain slice model capturing infiltration and transcriptional heterogeneity from core to single-cell dispersion - Nature Protocols

This protocol describes Core2Edge, a human glioblastoma organoid and brain slice co-culture model that recapitulates tumor infiltration and transcriptional heterogeneity, along with a light-sheet imag...

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A programmable DNA tetrahedron platform for selective and efficient capture of cells and proteins - Nature Protocols

This Protocol Extension describes a programmable tetrahedral DNA nanostructure (TDN) platform for selective cell and protein capture, using aptamer-functionalized TDN for cell capture and peptide-func...

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Three-dimensional tracking of dynamic structures in living cells using single-plane imaging with focus feedback - Nature Protocols

A microscopy approach for the use of focus-feedback for single-plane tracking of subcellular structures during prolonged live-cell fluorescent imaging sessions.

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Comprehensive quantitative analysis of polyolefin hydrogenolysis toward plastic waste management - Nature Protocols

Polymer deconstruction, via catalytic hydrogenolysis, is an important approach for plastic recycling and upcycling. This workflow describes sequential quantitative analysis using multiple techniques t...

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Protocols for immunological research

A collection of protocols covering a range of techniques for isolating, profiling, identifying and characterizing immune cells and biomarkers

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Tutorial: guidance on the use of large language models for medical research - Nature Protocols

An entry-level guide for understanding how to choose large language models for medical research applications, customize the prompts and fine-tune the models’ performance.

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Formulation and activation of lipid-shelled nanobubble ultrasound contrast agents - Nature Protocols

A protocol covering the synthesis of lipid-shelled nanobubbles, which enable an increase in sensitivity of contrast-enhanced ultrasound imaging and expand the possible applications in the clinical spa...

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Identifying lipid–protein interactomes with functional lipid probes - Nature Protocols

Cellular lipids are known to have specific protein interactions. This protocol describes how to study the lipid–protein interactome using multifunctional lipid probes capable of photo-cross-linking an...

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Fabrication and characterization of optoelectronic in-sensor computing devices - Nature Protocols

Optoelectronic devices based on two-dimensional layered thin film semiconductors can be tuned to directly sense and process spatial and temporal information, making them suitable to use as in-sensor c...

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Spatial imaging protocols

A collection of Protocols covering a wide variety of spatial imaging and spatial omics approaches. The collection aims to facilitate the adoption of high ...

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Tissue clearing protocols

A collection of Protocols and Tutorials covering a variety of approaches for the optical clearing of tissue samples

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A scalable high-throughput serolomics platform for profiling serum antibody responses in large-scale population-based cohorts - Nature Protocols

In this Protocol, the authors describe their automated multiplex serology workflow for large-scale population-based cohorts, an automated assay platform that robustly quantifies antibodies against up ...

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Preparation of solid-state emissive carbon quantum dots and their integration into electroluminescent light-emitting diodes - Nature Protocols

The use of carbon quantum dots (CQDs) in LED technologies is limited owing to aggregation-induced quenching in the solid state. Here, solid-state emissive CQDs with architectures that mitigate this qu...

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Preparation of homogeneous ZnSeTeS quantum dots - Nature Protocols

Quantum dots are used in many display and imaging applications. Preparing blue quantum dots that are heavy-metal-free has been challenging. This Protocol describes the tunable synthesis of ZnSeTeS qua...

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Automated cGMP optical labeling of FDA-approved antibodies for human use - Nature Protocols

This Protocol describes an automated approach for optical labeling of therapeutic monoclonal antibodies by using disposable commercial components, yielding a cGMP-grade product at reduced cost and wit...

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Rigid polyanion architectures enable multiple ion-transport pathways and nanocluster dynamics in composite polymer battery electrolytes - Nature Nanotechnology

Distinct lithium-ion conduction mechanisms in composite polymer battery electrolytes based on rigid polyanion architectures are identified and quantitatively characterized, providing insight into ion-...

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Whole-organ spatial transcriptional analysis at cellular resolution using TRISCO - Nature Protocols

We present a method for performing RNA in situ hybridization in intact organs that enables the spatial mapping of RNA transcripts in large samples, cleared and imaged using light-sheet microscopy.

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Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants - Nature Protocols

Europium chelate-labeled micro- and nanoplastics can be used to determine what parameters affect their uptake and biodistribution in different plant species. This protocol integrates fluorescence and ...

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High-throughput measurements of protein domain functions using magnetic separation - Nature Protocols

This Protocol presents a scalable magnetic separation-based method for screening large protein domain libraries in mammalian cells, providing a simple and quantitative alternative to fluorescence-acti...

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Tutorial: strategies for targeting organelles using nanoparticles - Nature Protocols

In this Tutorial, the authors describe strategies for designing nanoparticles that target key organelles, including mitochondria, lysosomes, the nucleus, the endoplasmic reticulum and the Golgi appara...

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Tutorial: a beginner’s guide to fast neutron detection with an EJ-309 detector - Nature Protocols

A Tutorial to make accessible the measurement of neutron yield and energy to researchers, enabling the pursuit of fusion reactor prototypes and their development.

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Tutorial: biomembranes in hybrid living bioelectronics - Nature Protocols

This is a Tutorial Review on advances in bioelectronic interfaces covering research in material science for biomimetic interfaces and in synthetic biology for the reconstitution of complex biological ...

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Tutorial: strategies for targeting organelles using nanoparticles

Nature Protocols, Published online: 08 July 2026; doi:10.1038/s41596-026-01401-6In this Tutorial, the authors describe strategies for designing nanoparticles that target key organelles, including mitochondria, lysosomes, the nucleus, the endoplasmic reticulum and the Golgi apparatus.

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CODAvision: best practices and a user-friendly interface for rapid, customizable segmentation of medical images

Nature Protocols, Published online: 07 July 2026; doi:10.1038/s41596-026-01404-3The semantic segmentation of microanatomical structures can be customized for the analysis of a diverse range of biomedical image modalities, including histology, magnetic resonance imaging and computed tomography.

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Preparation of placenta-tropic mRNA lipid nanoparticles for pregnancy disorders - Nature Protocols

We present a Protocol for the preparation of placenta-tropic mRNA lipid nanoparticles (LNPs), evaluation of the effects of protein adsorption on mRNA transfection in vitro and assessment of LNP biodis...

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Preparation of targeted lipid nanoparticles for precision nucleic acid delivery - Nature Protocols

This protocol outlines the steps to prepare functionalized lipid nanoparticles covalently conjugated with whole antibodies or antibody fragments for targeted nucleic acid delivery to different tissues...

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Oscillatory brain state-dependent stimulation with transcranial magnetic stimulation combined with electroencephalography - Nature Protocols

An approach that couples the application of transcranial magnetic stimulation with brain oscillations to improve the effectiveness of long-term plasticity induction.

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Single-cell ultra-high-throughput multiplexed chromatin accessibility and gene expression sequencing (SUM-seq) - Nature Protocols

A protocol for simultaneous profiling of chromatin accessibility and gene expression in single nuclei that permits sample multiplexing via sample-specific in situ barcoding and high-throughput droplet...

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