My first-author PhD paper is out in @natcomms.nature.com 🙌 We developed unique in vivo/ex vivo live-imaging pipelines to uncover new muscle stem cell behaviors in #DuchenneMuscularDystrophy. Big thanks to all co-authors & the @tajbakhshlab.bsky.social lab! www.nature.com/articles/s41...

Impaired stem cell migration and divisions in Duchenne muscular dystrophy revealed by live imaging - Nature Communications

Using live-imaging in vivo and ex vivo, Sarde et al. show that dystrophic muscle stem cells have precocious differentiation through symmetric divisions and impaired migration, with differential impact...

nature.com

@devstempasteur.bsky.social @institutpasteur.bsky.social @cnrsbiologie.bsky.social

Tajbakhsh Lab@tajbakhshlab.bsky.social · 7mo ago

Bravo Liza, Brendan and colleagues for the elegant work on multiple live imaging strategies to untangle mechanisms of muscular dystrophy! www.nature.com/articles/s41...

Bravo Liza, Brendan and colleagues for the elegant work on multiple live imaging strategies to untangle mechanisms of muscular dystrophy! www.nature.com/articles/s41...

Impaired stem cell migration and divisions in Duchenne muscular dystrophy revealed by live imaging - Nature Communications

Using live-imaging in vivo and ex vivo, Sarde et al. show that dystrophic muscle stem cells have precocious differentiation through symmetric divisions and impaired migration, with differential impact...

nature.com

Now published in its final version! Congrats again to all the authors for the amazing work 👏 www.nature.com/articles/s41...

Impaired stem cell migration and divisions in Duchenne muscular dystrophy revealed by live imaging - Nature Communications

Using live-imaging in vivo and ex vivo, Sarde et al. show that dystrophic muscle stem cells have precocious differentiation through symmetric divisions and impaired migration, with differential impact...

nature.com

Brendan Evano@brendan-evano.bsky.social · last yr.

🔥🔥🔥 New preprint! Our new work explores the dynamic behaviour of muscle stem cells and their niche interactions in Duchenne Muscular Dystrophy. www.biorxiv.org/content/10.1... #MyoBlue

🎉Excited to see my master’s work contributed to this amazing paper! 🎥 🧠 Congrats to all co-authors 👏 Grateful for my time in Dr. Isabelle Caillé & Pr. Alain Trembleau’s lab #IBPS. Isabelle’s passion for science will keep inspiring me ✨ doi.org/10.1126/scia...

GPR161 mechanosensitivity at the primary cilium drives neuronal saltatory migration

Primary cilium senses mechanical cues to regulate young neuron migration via GPR161 signaling.

doi.org

Unique opportunity to join the CytoMorpho Lab (in Paris ❤️) as a postdoc to work on the self-organization and polarization of reconstituted networks made of microtubules, motors and actin filaments in vitro. Experience required. Please Repost 🙏 thanks !

A work that took years to implement several quantitative live imaging pipelines. Worth the wait!! Beautiful results that give new insights into normal and dystrophic myogenic cell behavior and their dialogue with the niche. Kudos Liza, Brendan and colleagues.

Liza Sarde@sardeliza.bsky.social · last yr.

Excited to share that the preprint of my Ph.D. work is now available! 🎉 Huge thanks to my amazing co-authors for their support and collaboration: @gaellel.bsky.social, H. Varet, V. Laville, J. Fernandes, @tajbakhshlab.bsky.social, @brendan-evano.bsky.social. @devstempasteur.bsky.social @pasteur.fr

Excited to share that the preprint of my Ph.D. work is now available! 🎉 Huge thanks to my amazing co-authors for their support and collaboration: @gaellel.bsky.social, H. Varet, V. Laville, J. Fernandes, @tajbakhshlab.bsky.social, @brendan-evano.bsky.social. @devstempasteur.bsky.social @pasteur.fr

Impaired stem cell migration and divisions in Duchenne Muscular Dystrophy revealed by live imaging

Dysregulation of stem cell properties is a hallmark of many pathologies, but the dynamic behaviour of stem cells in their microenvironment during disease progression remains poorly understood. Using t...

biorxiv.org

🔥🔥🔥 New preprint! Our new work explores the dynamic behaviour of muscle stem cells and their niche interactions in Duchenne Muscular Dystrophy. www.biorxiv.org/content/10.1... #MyoBlue

Impaired stem cell migration and divisions in Duchenne Muscular Dystrophy revealed by live imaging

Dysregulation of stem cell properties is a hallmark of many pathologies, but the dynamic behaviour of stem cells in their microenvironment during disease progression remains poorly understood. Using t...

biorxiv.org

Static methods have reported conflicting results about dystrophic stem cells. But actually capturing their temporal dynamics is really key to understand critical aspects of the disease!

👏👏 Amazing work @sardeliza.bsky.social, @gaellel.bsky.social , H. Varet, V. Laville, J. Fernandes, @tajbakhshlab.bsky.social @devstempasteur.bsky.social @pasteur.fr @cnrsbiologie.bsky.social Key findings 👇

Using in vivo live imaging, we showed that dystrophic stem cells have impaired migration kinetics and precocious differentiation.

We developed an ex vivo live imaging assay with stem cells within their muscle fibre niche to recapitulate the in vivo phenotypes and allow for perturbation studies.

Ex vivo dystrophic stem cells indeed showed altered migration and precocious differentiation, through imbalanced symmetric divisions.

To explore the contribution of autonomous vs niche-driven cues, we set up a niche switching assay between healthy and dystrophic stem cells and muscle fibres. (YFP-labelled dystrophic stem cell grafted to a healthy fibre here)

Stem cell fate decisions were cell-autonomous, while their migration behaviour was largely determined by the healthy vs dystrophic muscle fibre niches.

For mechanistic studies we developed an in vitro live imaging assay with isolated stem cells.

The differentiation of dystrophic stem cells relied on dual p38–PI3K signalling, an unrecognised synergy not seen in healthy cells which depended solely on p38.

Our findings set Duchenne Muscular Dystrophy also as a stem cell disease, prior to the well-established muscle fibre fragility. Hope you'll like it, feedback much appreciated!