We're launching a major collaborative Center aimed at accelerating phage therapy @gladstoneinst.bsky.social. High-throughput experiments with engineered phages (my lab) and strains (Silas Lab), organoids (Ott Lab), and AI (Pollard Lab). gladstone.org/news/gladsto...

Gladstone Launches Center for PhAIge Therapy to Harness AI in the Fight Against Drug-Resistant Infections

The center, funded by an NIH grant, will become one of three national centers dedicated to accelerating the development of phage therapy.

gladstone.org

Out now in Nature Biotechnology! Alejandro González-Delgado and a fantastic team of collaborators worked across nine labs to get retron recombineering up-and-running in fifteen bacterial species. Molecular parts on addgene: www.addgene.org/browse/artic... Happy editing!

Nature Biotechnology@natbiotech.nature.com · 3mo ago

Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering - @seth-shipman.bsky.social @gladstoneinst.bsky.social go.nature.com/4tZluOU

New preprint on Detectrons from @jihoon-han.bsky.social! These are programmable biosensors for RNA that produce DNA barcodes in the presence of their target RNA. Check out Jihoon's quoted thread and the preprint for more: www.biorxiv.org/content/10.6...

Jihoon Han@jihoon-han.bsky.social · 6mo ago

Excited to share a preprint from my postdoc work in Shipman’s lab! We developed Detectrons — programmable biosensors that convert RNA signals into stable DNA barcodes inside living cells, enabling multiplexed RNA sensing. 🧵👇 biorxiv.org/content/10.6...

Been working on a really strange retron bacterial immune system, here's the preprint: www.biorxiv.org/content/10.1... Type VI retrons are unlike any other. Phage infection triggers reverse transcription of a DNA fragment that activates translation of a toxin to kill the infected cell.

Now published in PLOS Biology! We found new retron containing bacteria in the wild, figured out defense mechanisms, and turned them into genome editors. From a cupful of dirt to new parts for genome editing in one story! journals.plos.org/plosbiology/...

New retron systems from environmental bacteria identify triggers of anti-phage defense and expand tools for genome editing

Bacteria rely on retrons for phage defense, yet most known systems come from clinical or lab strains. This study identifies new and diverse retrons from environmental bacteria, uncovering their phage ...

journals.plos.org

Seth Shipman@seth-shipman.bsky.social · 2y ago

We just posted a new preprint where we found retrons in bacteria out in the real world, in dirt and water. We describe the first retrons in a handful of new species, show how they defend against phages, and use them to edit genomes. Read about it here: www.biorxiv.org/content/10.1...

Check out MitoScribe in our new preprint led by Linhan Wang: www.biorxiv.org/content/10.1... It's an analog molecular recorder that uses neutral base edits to the mitochondrial genome to store information about historical signaling in a cell. Single cell resolution at scale (see next post)!

New Preprint!! Alejandro González-Delgado accomplished a major feat on this one: ported retron recombineering, which we love so much in E. coli, into 14 new bacterial species via a massive collaborative effort involving 9 labs! www.biorxiv.org/content/10.1...

We just posted a new preprint where we found retrons in bacteria out in the real world, in dirt and water. We describe the first retrons in a handful of new species, show how they defend against phages, and use them to edit genomes. Read about it here: www.biorxiv.org/content/10.1...

🚨New Pub: High throughput variant libraries and machine learning yield design rules for retron gene editors, now out in Nucleic Acids Research. This is a great one, and the last bit of Kate Crawford's PhD, who is off to David Bakar's Lab for a postdoc. academic.oup.com/nar/advance-...

High throughput variant libraries and machine learning yield design rules for retron gene editors

Abstract. The bacterial retron reverse transcriptase system has served as an intracellular factory for single-stranded DNA in many biotechnological applica

academic.oup.com