More structural biology! From the @jburkevic.bsky.social lab with work from @sushantsuresh.bsky.social and @alexlouiseshaw.bsky.social - featuring data collected in our facility! #cryoEM www.jbc.org/article/S002...

Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA

Phosphatidylinositol 4 kinase IIIα (PI4KIIIα/PI4KA) is an essential lipid kinase that plays a critical role in regulating plasma membrane identity. PI4KA is primarily recruited to the plasma membrane ...

jbc.org

Final part of my PhD work is now out! We were able to develop a nanobody that inhibits EFR3 mediated PI4KA membrane recruitment. Potential therapeutic idea? Amazing collaboration with different labs using a host of different techniques. @alexlouiseshaw.bsky.social

John Burke@jburkevic.bsky.social · 9mo ago

Cool new paper (www.jbc.org/article/S002... from my lab spearheaded by graduate students Sushant @sushantsuresh.bsky.social and Alex @alexlouiseshaw.bsky.social . This was a very cool collaboration with the Scott Hansen, Jennifer Cobb, Tamas Balla labs

Great collaborative work where we developed a nanobody based tool to block EFR3 mediated recruitment of PI4KA. A potential therapeutic window maybe? Thanks @alexlouiseshaw.bsky.social for the assist!

Alexandria Shaw@alexlouiseshaw.bsky.social · last yr.

New preprint from the lab headed up by @sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!

New preprint from the lab headed up by @sushantsuresh.bsky.social! Late last year, we published the molecular basis of PI4KA plasma membrane recruitment by EFR3 (PMID:39705356). This work builds on these findings and identifies a new potential therapeutic avenue! Check it out!

bioRxivpreprint@biorxivpreprint.bsky.social · last yr.

Development of an inhibitory TTC7B selective nanobody that blocks EFR3 recruitment of PI4KA https://www.biorxiv.org/content/10.1101/2025.07.28.667261v1

Back from vacation and really glad to share some work from my PhD research. Great collaborative effort @alexlouiseshaw.bsky.social Check out this post from my PI to learn more about Lipid kinase PI4KA and its regulation!

John Burke@jburkevic.bsky.social · 2y ago

First post up, sharing some cool work from my lab by @alexlouiseshaw.bsky.social and @sushantsuresh.bsky.social out today in Science Advances (www.science.org/doi/10.1126/...)