Topical formulation of repurposed FDA-approved compounds inhibits Pseudomonas aeruginosa ExoU and improves corneal infection outcomes

Author summary Pseudomonas aeruginosa is a leading cause of microbial keratitis, a sight-threatening infection characterised by rapid tissue destruction. A major contributor to disease severity is the...

dx.plos.org

Salmonella effector kinase SteC is activated by phosphorylation at Serine 379

Author summary Salmonella is a bacterium that causes a wide range of human diseases, from gastroenteritis to systemic infection. Blood stream infections are more common in young children or those who ...

dx.plos.org

Enteropathogenic Escherichia coli-mediated fast and coordinated Ca²+ responses regulate NF-κB activation

Through low levels of extracellular ATP, EPEC induces small, rapid and coordinated Ca2+ responses in epithelial cells, a yet unreported pattern, that down-regulate inflammation at the onset of infecti...

doi.org

Host–microbiota–pathogen interactions drive Salmonella enterica serovar Typhimurium pathogenesis - Nature Reviews Microbiology

In this Review, Vázquez-Torres discusses our current understanding of how the dynamic interplay between microbial competition, host defences and Salmonella enterica serovar Typhimurium countermeasures...

nature.com

Structural insights into the assembly mechanism of the molecular bushing in the bacterial flagellar motor - Communications Biology

CryoEM analysis of the P-ring on polyrod gives insights into the self-assembly mechanism of the bacterial flagellar motor as to how the P-ring forms around the rod with tight attachment and then detac...

nature.com

Evolution of N‐terminal mechanical lability as a determinant for Type <fc>III</fc> secretion

Many Gram-negative pathogens critically depend on the Type III secretion system (T3SS) to inject effector proteins into host cells for colonization. Because the channel of the T3SS is narrow (~2 nm),....

onlinelibrary.wiley.com

Identification of protein secretion systems and type III effectors in wood-associated bacteria of the genus Xylophilus | Microbiology Spectrum

Xylophilus species include poorly characterized plant-associated bacteria, among them the grapevine pathogen Xylophilus ampelinus, yet their molecular mechanisms of host interaction have remained larg...

journals.asm.org

Infection strategies of Salmonella Typhimurium for gut-lumen colonization: Overcoming host defenses, exploiting host responses, and adapting to the enteric niche

Diarrheal disease is a leading cause of child morbidity and mortality globally, largely resulting from contaminated food and water and exposure to enteric pathogens. Salmonella enterica serovar Typ...

tandfonline.com

Salmonella SopB suppresses post-transcriptionally regulated cytokine release to reduce early tissue inflammation and delay disease progression - Nature Communications

Salmonella enterica serovar Typhimurium is a major bacterial pathogen that causes intestinal disease by invading host tissues and manipulating cellular functions. In this study, researchers investigat...

nature.com

Virotrap Reveals Salmonella SopB as A Ubiquitinated Cargo for Host ESCRT-0

The pathogenic bacterium Salmonella survives and replicates in host cells within a Salmonella-containing vacuole (SCV), a membranous niche it actively remodels via secreted effectors. The phosphoinosi...

biorxiv.org

A secreted endosymbiont protein essential for colonizing host cells - Nature

SyeA was present in the Buchnera ancestor, is secreted into the host cytoplasm, is homologous to secreted effectors of bacterial pathogens and is essential for Buchnera transmission.

nature.com

Stress-adapted codon usage enables efficient virulence-gene expression in Salmonella

Virulence genes in bacterial pathogens are often A/T-rich and horizontally acquired, yet they must be translated efficiently under stress and nutrient limitation encountered during infection. Here we ...

biorxiv.org

Human-restricted Salmonella exploit preassembled flagella for intracellular motility and vacuolar escape

Human-restricted typhoidal Salmonella enterica serovars cause systemic infections known as enteric fever, whereas most non-typhoidal serovars cause self-limiting gastroenteritis. Both groups rely on f...

biorxiv.org

biorxiv.org

Structure of the proton-powered secretion motor at the heart of the bacterial flagellum

Bacterial type III secretion systems export proteins across the inner membrane using the proton-motive force (PMF), but how proton flow is coupled to opening of the export channel is unknown. Here we ...

biorxiv.org

The Yersinia pestis virulence effector YopM binds the human pyrin death domain to inhibit inflammasome activation and effector-triggered immunity

Bacteria in the genus Yersinia use a type III secretion system to inject several effectors into host cells to disrupt multiple signaling processes. Two effectors disrupt host signaling by inactivating...

biorxiv.org

Flagellar system translocates T3SS effectors critical for Salmonella infection

Type 3 secretion system (T3SS) injectisomes, the essential virulence factors of many bacterial pathogens, are thought to have evolved from the flagellar T3SS. Despite their high degree of structural s...

biorxiv.org

Mechanobiological mechanisms of bacterial infection-host defense interactions

Recent advances in infection biology have increasingly underscored that mechanical forces and microenvironmental cues shape host-pathogen interactions…

sciencedirect.com

Phenotypic heterogeneity optimizes trade-offs during adaptive deployment of the type VI secretion system

The type VI secretion system (T6SS) is a widespread nanoweapon deployed by bacteria, but why is its deployment heterogeneous in many species? This study of T6SS in Escherichia coli shows that adopting...

dx.plos.org

biorxiv.org

Lithocholic acid induces T3SS-dependent formation of invasion-competent Shigella flexneri aggregates | Infection and Immunity

Shigella spp. are the causative agents of bacillary dysentery, or shigellosis, a highly contagious diarrheal disease characterized by abdominal cramps, fever, severe diarrhea, and stools containing mu...

journals.asm.org

biorxiv.org

Flagellin beyond motility: exploiting flagellar filaments in biotechnology

Flagellin is the structural protein of the bacterial flagellar filament, an extracellular propeller that rotates to drive swimming in many bacteria. The structure of flagellin allows for outer domain ...

cell.com

LuxS/AI-2 regulates phoP/phoQ by a non-canonical mechanism to enhance acid stress survival in Salmonella Typhimurium

Author summary To survive and cause disease within the host, enteric pathogens such as Salmonella sense and respond to changing environmental cues. One key strategy is quorum sensing, a cell–cell comm...

dx.plos.org

biorxiv.org