Following the PLK1 datasets, the Maltose Binding protein data from the same study is now available on EMPIAR. www.ebi.ac.uk/empiar/EMPIA... Now that both datasets are accessible, it’s a great opportunity for those interested in small protein cryo-EM processing to evaluate or test their methods.

So it looks like only the PLK1 kinase domain–onvansertib datasets are available right now. Not sure about the MBP datasets, but if you’re interested in small-protein cryo-EM processing, it might still be worth taking a look at the currently available data. www.ebi.ac.uk/empiar/EMPIA...

‘CryoDUCK Tokyo Days’ day 1 off to a great start. Really nice catching up with familiar faces, and finally meeting in person people I had only known online. And somehow we already got a Tokyo Skytree reference in the slides on the first day

The early release version of our #cryoEM work on CDK11-cyclin L-SAP30BP has now been published at Nature Communications: www.nature.com/articles/s41... We got very helpful comments from the reviewers and added more structural analysis and biochemistry to support our conclusions. Please have a look!

Basil Greber@bjgreber.bsky.social · 4mo ago

Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation. www.biorxiv.org/content/10.6...

Apoferritin: 1.67 Å from the ~3,600 micrographs (~21k by collected/Krios G4, Falcon4i, SelectrisX) Collected and processed a subset in some spare time, with some help from Claude. Still, it’s not quite there yet when it comes to picking good 2D classes or dealing with issues during processing.

The small protein cryo-EM paper I helped start has been accepted in Nature Communications - what a relief. Thanks to @landerlab.bsky.social and @olibclarke.bsky.social for their constructive review. Congratulations to all co-authors, and wishing everyone good luck. www.nature.com/articles/s41...

1. After going back through the RELION tutorial run with Claude and correcting several issues, the reconstruction reached 2.9 Å, matching the tutorial result. The first run had given 3.22 Å, which led me to look more carefully at what had happened in the processing. So I reviewed the workflow again.

it’s been a while. Since I have no local workstation for data processing in my new role yet, I’ve been playing with AWS. In my downtime, I asked Claude Code to follow a RELION tutorial from scratch. With a few fixes, I got a 3.22 Å result. Not bad for AI handling routine tasks! :P

Attending the Scipion workshop at KAIST. Looking forward to learning a lot over the next 4 days

Talented researchers from Rho lab at Seoul National University just published impressive results. Glad I could help with some of the cryo-EM data collection for this work. www.science.org/doi/10.1126/...

Structural dissection of αβ-tubulin heterodimer assembly and disassembly by human tubulin–specific chaperones

Microtubule assembly requires a set of chaperones known as tubulin-binding cofactors (TBCs). We used cryo–electron microscopy to visualize how human TBCD, TBCE, TBCC, and guanosine triphosphatase (GTP...

science.org

I think this will be my last post on this topic. I don’t want to keep talking about the authorship issue. Even though I wasn’t directly involved in the experiments/analyses for the paper, I believe this achievement wouldn’t have been possible without the discussions I led on SNS and the work

Just received this response about why I was excluded from authorship on the small protein cryo-EM paper "We have to respect the opinions of the members who actually conducted the research. What they confirmed is that the research was conducted after Dr. Kim left the company." 1/3

PNCC Cryo-EM modeling and validation workshop on Sep 3-5, 2025. Register now! tinyurl.com/Cryo-EMModel...

When I was at BaobabAiBIO, I was hesitant to publish the results because the PLK1 map was incomplete (I wrote about my concerns on Twitter). Before quitting my job, I tried to finish at least these 2 things: - getting a complete PLK1 map - uploading MBP/PLK1 data to EMPIAR

Oli Clarke@olibclarke.bsky.social · 12mo ago

This is an *excellent* review, and I hadn’t heard of PREreview before and I love the idea! On another note (as you & Gabe highlight) looking at the PLK1 map really brings home that we need mandatory reporting of some kind of anisotropy metric - that map is not buildable, & def not close to 3Å IMHO

BTW, Jan-Hannes Schäfer @jhschaef.bsky.social and Gabriel C. Lander @landerlab.bsky.social provided an excellent PREreview of the paper. I really appreciate it. prereview.org/reviews/1581...

Han Kim@trancekr.bsky.social · 12mo ago

Although I’m not on the author list, the MBP/PLK1 cryo-EM structure paper has been published on bioRxiv. FYI, I was not involved in any writing or reviewing of the paper www.biorxiv.org/content/10.1...

Although I’m not on the author list, the MBP/PLK1 cryo-EM structure paper has been published on bioRxiv. FYI, I was not involved in any writing or reviewing of the paper www.biorxiv.org/content/10.1...

High-resolution cryo-EM structures of small protein–ligand complexes near the theoretical size limit

Cryo-electron microscopy (cryo-EM) is widely used to determine macromolecular structures at atomic resolution. The theoretical size lower limit of particles for cryo-EM analysis is 38 kDa, limited by ...

biorxiv.org

I’m experiencing repeated errors during tomography data acquisition. Any suggestions on how to resolve this issue? Thanks in advance.

Thrilled to see my former lab's paper on bioRxiv. Their graphene-based affinity grid successfully resolved low-abundance protein complexes. After such a long journey, it's wonderful to see this project finally producing good results! www.biorxiv.org/content/10.1...

The graphene-based affinity cryo-EM grid for the endogenous protein structure determination

Following recent advancements in cryo-electron microscopy (cryo-EM) instrumentation and software algorithms, the next bottleneck in achieving high-resolution cryo-EM structures arises from sample prep...

biorxiv.org

As we head into 2025, let's take one final look back at 2024 and how the year unfolded in the PDB archive. There were 15319 entries released last year but what more can we find out about those structures ⚖️? A thread 1/11 🧵

Snow in Seoul

Come work with us! Only a few days remaining to apply for the #cryoET / #CLEM facility scientist position at the @icrlondon.bsky.social! #cryoEM jobs.icr.ac.uk/vacancies/96...

Higher Scientific Officer / Senior Scientific Officer – CLEM & cryoET scientist in Chelsea | The Institute of Cancer Research

View details and apply for this Higher Scientific Officer / Senior Scientific Officer – CLEM & cryoET scientist vacancy in Chelsea . Salary : Commencement on the salary range is subject to comp...

jobs.icr.ac.uk

New office desk & Krios G4 at SNU NCIRF

Starting from September, I will begin working at the Core Facility for Cell and Macromolecule Imaging and the National Center for Inter-university Research Facilities at Seoul National University to manage the cryo-EM equipment and provide research support.

Update on our kinase domain side project (37 kDa with a ligand): Previous sample had severe preferred orientation issue, pausing the project. Now back at it with new 2D classification results. Still preferred orientation persists, but we're optimistic about getting a map. keep the fingers crossed.

Our MBP cryo-EM structure is released. Next uploading the micrographs to EMPIAR. www.rcsb.org/structure/8YBE

Join us for the 2024 Cryo-EM Workshop for the pharma and biotech industries! Dive into the latest trends, techniques, and applications of Cryo-EM with experts from Baobab AiBIO and Thermo Fisher Scientific. (conducted in Korean / industry only) Mar 14, 10:00-17:00. docs.google.com/forms/d/e/1F...

Oli B. Clarke (I’m mot sure he is using bluesky or not) asked about the ice thickness of our MBP dataset. So I checked the relative ice thickness plot in cryoSPARC and compared our membrane protein dataset. Here is the result.

the first time using a multi-hole grid!!!

demoing Chameleon at Institute of Membrane Proteins/POSTECH

Sharing our IMC20 poster here. With all the feedbacks from twitter and IMC20, we will test more MBP samples internally and externally (with help from ThermoFisher). Stay tuned.