William Pearman

@williampearman.bsky.social

Research fellow at Auckland Uni. Wannabe bioinformatician, reluctant community ecologist. Evolutionary ecology, microbial ecology. Anglican. He/him

Anyone on here ever tried using a laser to break open really tough samples for DNA extraction? I'm working on some tough chitinous tissues which nothing seems to reliably lyse them - and thought I might take some inspiration from laser microdissection (which is a tad overkill/$$)

Has anyone here got much experience with microbial ecology and experimental pre-registration? I'm embarking on a new project, and would like to pre-register & peer review the experimental design - but I have no idea where to start.

I don't understand why people don't sequence negative extraction controls. I've spent more time recently managing reagent contamination for low biomass extractions than i've spent doing the actual extractions. Extensive sterile filtering, UV, and EMA treatment seemed to be the only way that worked.

Paul Orwin@paulmorwin.bsky.social · 2y ago

relevant to a paper I just reviewed (and cited this paper bmcbiol.biomedcentral.com/articles/10....). Ppl seem resistant to this lesson...

Am I right in assuming that blunting our humanities and social sciences research will dull our understanding of inequities suffered by Māori, and the role that a just Te Tiriti interpretation would play in resolving this? Because if so, it's hard not to connect the dots to the true intent here.

Eddie Clark@dreddieclark.bsky.social · 2y ago

"The focus of the Fund will shift to core science, with the humanities and social sciences panels disbanded and no longer supported." The changes are bad for science, they're basically fatal for meaningful govt support of humanities and social sciences. Blatant and unashamed anti-intellectualism.