Adrien Leger
@adrienleger.bsky.social
Director of Modified Bases Research at @nanoporetech.com EMBL-EBI alumni, keen cyclist and father of two. Views are my own. Reposts are not endorsement.
Our abstract submissions for London Calling 2026 are now open! If you’ve been using Oxford Nanopore technology to power the bigger and bolder research questions, we want to hear about it. Submit your abstract here: nanoporetech.com/about/events... #nanoporeconf
Excited to welcome @adrienleger.bsky.social from @nanoporetech.com to our RNA Symposium! 🎤 His talk will focus on how nanopore direct RNA sequencing can reveal multiple RNA modifications at once. Secure your spot at our symposium now 👉 zurl.co/mrhKq #RNA #ribosome #nanopore
Our paper using Oxford #Nanopore direct RNA sequencing to identify m6A modifications on RNA isoforms in human brain is now out in Science Advances. 🧪 www.science.org/doi/10.1126/...
Isoform-level profiling of m6A epitranscriptomic signatures in human brain
Direct RNA-seq in brain reveals RNA isoform and region-specific m6A modifications, highlighting their role in gene regulation.
science.org
🚨 We’re hiring ! 🚨 Join our cutting-edge research team as a molecular biologist at @nanoporetech.com HQ in Oxford. Perfect for a fresh PhD or MSc with a couple of years’ experience. Work at the interface of chemistry, molecular/synthetic biology and AI 👉 ejnh.fa.em2.oraclecloud.com/hcmUI/Candid...
The world prefers its energy more in the form of electricity than anything else.
At London Calling, Rosemary Sinclair Dokos and Lakmal Jayasinghe shared how we’re doubling down on what matters: delivering a future-ready platform with proven performance across DNA, RNA, and now proteins. Click here to discover what’s new – and why it matters: nanoporetech.com/news/london-...
#NanoporeConf Lakmal Jayasinghe ONT is the *only* sequencing technology that detects RNA modifications directly. They're now up to 8 modifications that can be detected simultaneously. [This is something I think ONT should be shouting from the rooftops every day] RNA barcoding is in beta testing.
New HAC models are 1.4Q more accurate - a 25% error reduction
Big improvements in modification calling accuracy - 6mA increases from Q16 to Q24
Dorado v1.0.0 introduces dramatic improvement to modified base calling speed
Base calling obeys scaling laws in deep learning - a Q28 base caller using standard chemistry is now available on demand.
Oxford Nanopore sequencing will be ubiquitous for multiomics….and the next step is proteomics. First panel-based proteomics assays, before driving towards full protein sensing in the future. #nanoporeconf
Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf github.com/nanoporetech...
Dorado 1.0 🚀release with new RNA modified base models for 2’Ome as well as improvement of all DNA and RNA modified base models including massive improvement in 6mA for open chromatin prediction! Also a lot more non-mods related features😆! #NanoporeConf github.com/nanoporetech...
With significant ML advances, Dorado & MinKNOW now power built-in accurate variant calling, default methylation detection & short fragment mode. Adaptive sampling & T2T assemblies are production-ready — enabling faster, richer, more meaningful insights.
Dorado is 1.0! 🎉 LC25 release adds v5.2 DNA/RNA models with a new suite of mod models that are higher accuracy, lower FP rates, and significantly faster. - Introducing dorado variant 👀 - HAC error rate is down over 25%. - New 2’Ome mod models - Hopper & Blackwell SUP speed ups #Nanoporeconf
Really pleased that this paper is out - academic.oup.com/neuro-oncolo... thanks to all our collaborators far and wide who have helped and of course @nanoporetech.com
ROBIN: A unified nanopore-based assay integrating intraoperative methylome classification and next-day comprehensive profiling for ultra-rapid tumor diagnosis
academic.oup.com
Excited for another London Calling! Incredible updates coming over the next two days! #NanoporeConf @nanoporetech.com
I am quite interesting in this Nm modification. As we detected Nm in rRNA as well as mRNA using Nanopore RNA002 version. www.cell.com/molecular-ce...
2′-O-methylation at internal sites on mRNA promotes mRNA stability
Li et al. develop a machine learning approach to measure the stoichiometry of 2′-O-methylation at internal sites of mRNA using nanopore RNA sequencing data. FBL-mediated Nm modifications, when connect...
cell.com
I expect Rosemary and Lakmal (the new R&D team leads) will reveal more than just all this - I am looking forward to the reports on Wednesday and looking forward to catching up with everyone nanopore on Friday!
Nanopore now can call 4 RNA modifications - m6A (I love how DNA and RNA have different writing conventions - molecular biologists, just don't go changing!) - m5C, pseudourodine, and inosine. This is all new stuff, and there quite a bit on truth sets, sensitivity/specificity to play out
The final, most exotic stuff is RNA - so called "direct RNA" where modifications of the RNA is the really exciting stuff (the sequence itself, including intron/exon structure is probably still done best via cDNA, "old school").
Basically molecular biologists can either use incorporation (eg, BrdU) or modification (eg, bacterial enzymes tethered to antibodies) or chemical modification (eg, radicals etc) and then the read out is the DNA (or RNA, see below).
Nanopore can read other mods as well - hydroxymethylation, the oxidation product of methylation which is only really present in neuronal tissue in vertebrates (watch this space for insights on this - we will be preprinting soon!) and bacterial DNA methylation, like 6mA.
This week is the gathering of the tribes around @nanoporetech.com, for their annual meeting, London Calling. It is a blend of tech announcements, product updates + scientific conference (posters and breakout talks). I am only there for the informatics day at the end sadly, but here are some thoughts
Live from London Calling 2025! Speakers, attendees and the Oxford Nanopore crew are starting to fill into Old Billingsgate! Ready for Gordon to kick things off for the 11th time! Make sure you keep your posts coming using: #nanoporeconf
We are only hours away from the regular Tech update from Oxford @nanoporetech.com (18.15 UK time on Wednesday)
Now available as preprint: The ENCODE 4 expanded registry of regulatory elements - 2.35M 🧍 human cCREs - 927k 🐭 mouse cCREs www.biorxiv.org/content/10.1... Led by @moorejille.bsky.social, this preprint summarizes data and analyses generated by hundreds of contributors across ENCODE 4
