The paper describing our sensitive poly-ADPr antibodies and a few other things is now out in Nature Communications. Congrats to all the authors!! www.nature.com/articles/s41...

Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling - Nature Communications

ADP-ribosylation regulates DNA repair, but its distinct forms have been difficult to distinguish. Here, the authors develop sensitive, modular antibodies to precisely detect mono- and poly-ADP-ribosyl...

nature.com

The Matić Lab@maticlab.bsky.social · 8mo ago

We’ve made the poly-ADPr antibody AbD64138 available in a SpyTag HRP-conjugated format (TZA0117P): www.bio-rad-antibodies.com/monoclonal/h... This sophisticated format enables detection of very low poly-ADPr levels while simplifying and reducing the cost of detecting abundant poly-ADPr.

Superb News&Views on our recent paper reporting identification of ADP-ribosyl-linked serine ubiquitylation www.nature.com/articles/s41...

ADPr gets a ubiquitin upgrade - Nature Chemical Biology

MARUbylation is a hybrid post-translational protein modification in which mono-ADP-ribosylation acts as a scaffold for sequential mono- and polyubiquitylation. Recent studies illuminate its substrates...

nature.com

At its core, it’s a technological paper—necessary to overcome some of the many challenges of working with ADPrUb. But beyond the technical details, below are the main highlights:

The Matić Lab@maticlab.bsky.social · last yr.

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. A proteomics approach is developed using these domains to map ADP-ribosyl-ubiquitylation sites www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

nature.com

Thrilled to share my first first-author postdoc publication — a team effort with @aklvnbch.bsky.social. We present the first MS-based evidence of ADP-ribosylation–linked ubiquitin (ADPrUb) in cells after DNA damage. Grateful for all I’ve learned and looking forward to uncovering more about ADPrUb

The Matić Lab@maticlab.bsky.social · last yr.

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Really happy that our paper is finally released. We show that PARP1 and histones are targets of ADP-ribosyl-linked ubiquitylation during DNA damage and describe how to detect the dual modification. Great teamwork with @mariapalumbieri.bsky.social & thanks to everyone involved @maticlab.bsky.social !

The Matić Lab@maticlab.bsky.social · last yr.

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Thrilled to have presented our latest work at FEBS PARP 2025! A fantastic joint effort with @aklvnbch.bsky.social . I really enjoyed sharing our results and discussing with experts in the field—looking forward to what’s next! Many thanks to the organizers for making this great meeting possible!

We’ve uploaded a bioRxiv preprint on new antibodies specific for poly-ADP-ribosylation (PARylation). These modular tools were developed and validated through novel applications of our Serine ADP-ribosylation-based technology. www.biorxiv.org/content/10.1...