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The Matić Lab

@maticlab.bsky.social

Discovery and development of serine ADP-ribosylation. Proteomics and molecular tools for unexpected discoveries in signaling, the DNA damage response and aging Leibniz Institute on Aging - Fritz Lipmann Institute, Jena, Germany

24 Posts120 Follower90 Folgt
The Matić Lab@maticlab.bsky.social · last wk.

Thrilled to have joined the FLI as a senior group leader, and the FSU as a W3 Professor. Our journey of technology and discovery continues, now with access to outstanding infrastructure, including for ADP-ribosylation proteomics. Looking forward to welcoming new colleagues and collaborators!

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Leibniz-Institut für Alternsforschung - Fritz-Lipmann-Institut@leibnizfli.bsky.social · last wk.

🤗 A warm welcome to Prof. Ivan Matić! 💐 Yesterday, Prof. Ivan Matić, who joined us in #Jena from @mpiage.bsky.social, began his work at @leibnizfli.bsky.social to establish and lead the new #research #group “Cell Signaling and Proteomics of Aging”. ➡️ We interviewed him: 👉 bit.ly/3T97d5t

The Matić Lab@maticlab.bsky.social · 5mo ago

The paper describing our sensitive poly-ADPr antibodies and a few other things is now out in Nature Communications. Congrats to all the authors!! www.nature.com/articles/s41...

Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling - Nature Communications

ADP-ribosylation regulates DNA repair, but its distinct forms have been difficult to distinguish. Here, the authors develop sensitive, modular antibodies to precisely detect mono- and poly-ADP-ribosyl...

nature.com

The Matić Lab@maticlab.bsky.social · 7mo ago

We’ve made the poly-ADPr antibody AbD64138 available in a SpyTag HRP-conjugated format (TZA0117P): www.bio-rad-antibodies.com/monoclonal/h... This sophisticated format enables detection of very low poly-ADPr levels while simplifying and reducing the cost of detecting abundant poly-ADPr.

Anthony Leung@leunglab.bsky.social · 5mo ago

ADP-ribosylation is far more diverse than the usual mono vs poly view. New sensitive tools from the Ivan Matic lab to resolve this complexity are a big step forward — expanding what more labs can now study. www.nature.com/articles/s41...

Versatile and sensitive detection of mono- and poly(ADP-ribosyl)ation reveals XRCC1-dependent remodelling of PARP1 signalling - Nature Communications

ADP-ribosylation regulates DNA repair, but its distinct forms have been difficult to distinguish. Here, the authors develop sensitive, modular antibodies to precisely detect mono- and poly-ADP-ribosyl...

nature.com

The Matić Lab@maticlab.bsky.social · 7mo ago

We’ve made the poly-ADPr antibody AbD64138 available in a SpyTag HRP-conjugated format (TZA0117P): www.bio-rad-antibodies.com/monoclonal/h... This sophisticated format enables detection of very low poly-ADPr levels while simplifying and reducing the cost of detecting abundant poly-ADPr.

anti POLY-ADP-RIBOSE Antibody, clone AbD64138 | Bio-Rad

Anti poly-ADP-ribose antibody, clone AbD64138, recognizes poly-adenosine diphosphate (ADP)-ribosylation. ADP-ribosylation is a reversible post-translational modification that occurs in multicellular o

bio-rad-antibodies.com

The Matić Lab@maticlab.bsky.social · 2y ago

We’ve uploaded a bioRxiv preprint on new antibodies specific for poly-ADP-ribosylation (PARylation). These modular tools were developed and validated through novel applications of our Serine ADP-ribosylation-based technology. www.biorxiv.org/content/10.1...

MPI for Biology of Ageing@mpiage.bsky.social · 11mo ago

We were delighted to have Nobel laureate Venki Ramakrishnan with us to discuss his exciting new book, "Why We Die." Thanks so much, Venki, for your fascinating and thought-provoking talk - it was truly a pleasure to have you join us!

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Nature Chemical Biology@natchembio.nature.com · last yr.

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. A proteomics approach is developed using these domains to map ADP-ribosyl-ubiquitylation sites www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

nature.com

Maria Dilia Palumbieri@mariapalumbieri.bsky.social · last yr.

Thrilled to share my first first-author postdoc publication — a team effort with @aklvnbch.bsky.social. We present the first MS-based evidence of ADP-ribosylation–linked ubiquitin (ADPrUb) in cells after DNA damage. Grateful for all I’ve learned and looking forward to uncovering more about ADPrUb

The Matić Lab@maticlab.bsky.social · last yr.

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Andreas Kolvenbach@aklvnbch.bsky.social · last yr.

Really happy that our paper is finally released. We show that PARP1 and histones are targets of ADP-ribosyl-linked ubiquitylation during DNA damage and describe how to detect the dual modification. Great teamwork with @mariapalumbieri.bsky.social & thanks to everyone involved @maticlab.bsky.social !

The Matić Lab@maticlab.bsky.social · last yr.

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

The Matić Lab@maticlab.bsky.social · last yr.

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

nature.com

Anthony Leung@leunglab.bsky.social · last yr.

New Paper💡PARP enzymes regulate ADP-ribosylation, a protein modification linked to cancer, infections & neurodegeneration. But it comes with two forms—MARylation (single) & PARylation (multiple). Meet dELTA-MS, a mass spec method that identifies both in one experiment! pubs.acs.org/doi/full/10....

dELTA-MS: A Mass Spectrometry-Based Proteomics Approach for Identifying ADP-Ribosylation Sites and Forms

ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MARylation) and polymeric (PARylation) forms. Little is known about the specific contribut...

pubs.acs.org

Mike Cohen@michaelnadbio.bsky.social · 2y ago

I'm super excited to share our study in collaboration with @jnpruneda.bsky.social, published today in @embojournal.org! We show that PARPs are modified with a non-canonical ester-linked mono-ADP-ribosylated ubiquitin species for the first time in cells: www.embopress.org/doi/full/10....

Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose | The EMBO Journal

imageimageCertain E3 ligases have been found to ubiquitylate hydroxyl groups on free NAD+ and ADP-ribose in vitro, but the in vivo occurrence of this dual post-translational modification has remained ...

embopress.org