Last week we had fun presenting our toxin research at the #EurekaScienceFestival at #ForumGroningen, together with Marloes Susan Eerelman and @dshatskiy.bsky.social . It was fantastic to see so many people interested in science, cryo-EM and toxin research! Thanks to the organizers!

A massive update: At least ~15 companies~ are selling scientists antibodies using faked validation data. We've documented 18,000+ manipulated images on 17,000+ products sold by leading laboratory suppliers including Thermo Fisher, Abcam, Santa Cruz Biotechnology, Millipore Sigma and Bio-Techne. 1/🧵

Are you excited to uncover the molecular mechanisms of actin filament assemblies that drive cell migration using cryo-EM? Only 1.5 week to apply for this exciting PhD position in my newly established lab at the Netherlands Cancer Institute in Amsterdam: www.werkenbijavl.nl/vacatures/ph...

PhD Student – Structural Biology of Actin Filament Assemblies | Werken bij AVL

werkenbijavl.nl

In our new article, we show how P. aeruginosa, a major cause of chronic respiratory infections in #cysticfibrosis (CF), uses the Type VI Secretion System #T6SS and specific #toxins to eliminate competing bacteria www.cell.com/cell-reports... #cryoEM @dshatskiy.bsky.social @jakecolautti.bsky.social

Thrilled to be one of 12 awardees of the prestigious #ERCStG from the LS1 panel this year! Over the next five years, we’ll study how intracellular bacterial pathogens #Listeria and #Shigella manipulate #actin to spread infection, with the aim of finding ways to stop them. Exciting times ahead!

Dr Ino Agrafioti@inoagrafioti.bsky.social · last yr.

WARM congratulations to: Tom DENDOOVEN, @martinpacesa.bsky.social, @mschuhmacher.bsky.social , @lfmilles.bsky.social, @preinerin.bsky.social,Markus Hopfler, @charleneboumendil.bsky.social, Gergo Gogl, @shifralansky.bsky.social, @alexanderbelyy.bsky.social,Erik Benson, @dbojar.bsky.social! 🤩 #ERCStG

🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins. Led by superstar @wahyuwidodo.bsky.social www.biorxiv.org/content/10.1... A 🧵👇🏽

If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”? If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein. Think I’ve finally found a way.

#Pseudomonas aeruginosa destroys other #bacteria using #T6SS and its effectors during infection in #cysticfibrosis patients. Here, we determined the #cryo-EM #structure of the effector Ptx2, which depolarizes target bacterial membranes. Check it out! www.biorxiv.org/content/10.1...

Structure of the F-tractin-F-actin complex. A new study by Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner @uni-muenster.de, and Alexander Belyy @alexanderbelyy.bsky.social @unigroningen.bsky.social: https://buff.ly/3EIqAur #Cytoskeleton #StructuralBiology #Biochemistry #Actin

New: Using F-tractin to visualize #actin in living cells? Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner, and Alexander Belyy @alexanderbelyy.bsky.social determined the F-tractin-F-actin structure and showed that this probe is nearly identical to #Lifeact. https://buff.ly/3EIqAur #cytoskeleton

Are you doing cell #biology? Then, let me guess, you likely stained #actin! Our @jcb.org article reveals the structure of the popular actin probe F-tractin in complex with F-actin and introduces an optimized version for better imaging! A thread! 👀👇 rupress.org/jcb/article/...

If you think you know everything about enzymes, check this out, we have a surprise for you 😃

Kasia Tych@biophysk.bsky.social · 2y ago

Proud to announce Yulia's paper in Nano Letters. We were planning to measure enzyme diffusion rates under different conditions using label-free single-molecule methods, and accidentally uncovered an interesting effect: pubs.acs.org/doi/10.1021/... 🧪