New preprint alert! 🦠🔬 We quantitatively evaluated the swimming and flagellar movement of Chattonella, a red-tide alga. Chattonella has two unequal flagella, and we found a positive correlation between anterior flagellum length and swimming speed. #protistonsky www.biorxiv.org/content/10.6...

Our paper on dynein-2's track recognition mechanism is now published in The EMBO Journal @embojournal.org!! Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet | The EMBO Journal www.embopress.org/doi/full/10....

Cryo-ET and MD simulations reveal that dynein-2 is tuned for binding to the A-tubule of the ciliary doublet | The EMBO Journal

imageimageDynein-2 is a motor protein essential for retrograde intraflagellar transport (IFT) on the A-tubule of ciliary doublet microtubules. Here, biochemical and cryo-ET analyses demonstrate that d...

embopress.org

Excited to share that I've joined EABIAS (East Asia Bioimage Analysts’ Society) following the recent GloBIAS Bioimage Analysis Conference 2025! Happy to be part of this community. よろしくお願いします＆請多指教！ eabias.org/about/

Safely back in Hokkaido! Had a wonderful time at #GloBIAS2025 in Kobe. The atmosphere of mutual support was fantastic! Huge thanks to everyone who interacted with me! (Our university mascot, Muropyon, enjoyed the beautiful Kobe night view too! 🌃) #GloBIAS

Going to Kobe for GloBIAS Bioimage Analysis Conference 2025! A bit at a loss what to wear, as we're already using the heater here in Muroran, Hokkaido.🥶 #GloBIAS

Reposting our microPublication now indexed in PubMed: We analyzed the movement of the unicellular green alga Chlamydomonas using #DeepLabCut, a deep learning-based tracking tool originally developed for animal behavior studies. pubmed.ncbi.nlm.nih.gov/40331205/ Original videos: 🧵 #protistsonsky

Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking - PubMed

Tracking eukaryotic flagella and cilia often requires manual clicking, even in the age of digital imaging. We developed an autotracking method using DeepLabCut, a CNN-based, marker-less tracking tool ...

pubmed.ncbi.nlm.nih.gov

Azusa Kage@azusakage.bsky.social · last yr.

Our analysis of the rotational movement of Chlamydomonas uniflagellar mutant using #DeepLabCut has just been published in microPublication Biology! micropublication.org/journals/bio...

Reposting our microPublication now indexed in PubMed: We analyzed the movement of the unicellular green alga Chlamydomonas using #DeepLabCut, a deep learning-based tracking tool originally developed for animal behavior studies. pubmed.ncbi.nlm.nih.gov/40331205/ Original videos: 🧵 #protistsonsky

Revealing Rotational Characteristics of the Uniflagellate Mutant of Chlamydomonas reinhardtii through DeepLabCut-Based Autotracking - PubMed

Tracking eukaryotic flagella and cilia often requires manual clicking, even in the age of digital imaging. We developed an autotracking method using DeepLabCut, a CNN-based, marker-less tracking tool ...

pubmed.ncbi.nlm.nih.gov

Azusa Kage@azusakage.bsky.social · last yr.

Our analysis of the rotational movement of Chlamydomonas uniflagellar mutant using #DeepLabCut has just been published in microPublication Biology! micropublication.org/journals/bio...

Spring in northern Japan🌸

Our analysis of the rotational movement of Chlamydomonas uniflagellar mutant using #DeepLabCut has just been published in microPublication Biology! micropublication.org/journals/bio...

Our analysis of the rotational movement of Chlamydomonas uniflagellar mutant using #DeepLabCut has just been published in microPublication Biology! micropublication.org/journals/bio...

Oxygen gradients shape E. coli motility patterns as they run away from O2! - 😍

bioRxiv Biophysics@biorxiv-biophys.bsky.social · last yr.

Spatial self-organization of confined bacterial suspensions https://www.biorxiv.org/content/10.1101/2025.02.20.639202v1

Asked similar question before but I try again. Anyone knows proteins that localize like this? This protein is present in diplonemids/bodonids/euglenids/Naegleria, which have two flagella, but not in trypanosomatids that have just one flagellum. Potentially interesting for flagella/cilia community?

Bungo Akiyoshi@bungoakiyoshi.bsky.social · 2y ago

The world is full of crazy and sad news, but here is a protein that localizes to only one of the two flagella

We have posted our new #preprint regarding the track recognition mechanism of dynein-2 that works in #cilia on bioRxiv @biorxivpreprint.bsky.social!! www.biorxiv.org/content/10.1... 1/8 🧵

Dynein-2 is tuned for the A-tubule of the ciliary doublet through tubulin tyrosination

Eukaryotic cilia and flagella are thin structures present on the surface of cells, playing vital roles in signaling and cellular motion. Cilia structures rely on intraflagellar transport (IFT), which ...

biorxiv.org

Dynein-2 is tuned for the A-tubule of the ciliary doublet through tubulin tyrosination https://www.biorxiv.org/content/10.1101/2025.01.27.635025v1

It’s interesting that all versions of Alphafold distort large extended proteins to ‘globularise’ them. It’s fine for people that understand those helices are ‘bent’ but for non experts it’s confusing. 🫤 🤔 It happens a lot and isn’t well discussed in the community.

【青山たけしSNS】 いつもSNSをチェックいただき ありがとうございます Xでの発信をメインとしてますが Instagramも 2000名近くの方に閲覧いただく （Xとは若干内容が異なります） Threadsに加えて新たに Bluesky bsky.app/profile/aoyama… をはじめました 今後も室蘭の情報発信を続けます

Proud to be part of this paper published in @naturecomms.bsky.social. Using high-speed AFM, we have visualized the real-time translocation of substrate polypeptide by the single molecule of SecYEG-SecA!!! Please check this amazing video taken by high-speed AFM!!! www.nature.com/articles/s41...

Happy New Year from the northside of Japan🌅🎍❄️

New Year decorations at our lab🎍

Did you know #diatoms are motile? These tiny glass cells have no cilia or flagella and a rigid body yet can glide on surfaces using a specialised slit in the cell wall called the raphe! What is the functional morphology of the raphe? All revealed in our new preprint www.biorxiv.org/content/10.1...

International Society of Protistologists is funding two symposia at the 16th International Congress of Protistology in Seoul, South Korea. Proposals are due 30 January 2025 #protist #protistsonsky protistologists.org/programs/fun...

Funding - International Society of Protistologists

Student support, seed money, and more. Got a tip for a program that funds protist research? Contact the Vice President. Society funding: The Holz-Conner Travel Fund provides funding for travel […]

protistologists.org

🍾Our small #UExM adventure is only starting! #ProtistsOnSky We are happy to announce that the Moore Foundation will be funding our efforts in creating an Expansion Microscopy atlas of #Microbial #Eukaryotes with @gautamdey.bsky.social @UNIGE @embl.org LINK: www.unige.ch/sciences/chi... 1/4

📢 Please re-share! New Preprint Alert -- in collaboration w/ @prannoysuraneni.bsky.social @coralprof.bsky.social and others @univmiami.bsky.social @miamirosenstiel.bsky.social FlumeX: A Modular Flume Design for Laboratory-Based Marine Fluid-Substrate Studies @SSRN papers.ssrn.com/sol3/papers....

Joined #NewPISlack 👀

12月1日から、国立遺伝学研究所の所長として赴任しました近藤滋です。 日本の基礎科学を盛り上げるために、精一杯働く所存です。皆さん、ご協力をお願いいたします。 所長挨拶： www.nig.ac.jp/nig/ja/about... 学生へのメッセージ： www.nig.ac.jp/nig/ja/phd-p... どちらも、楽しく読んでいただけるように書きました。特に、学生へのメッセージの方ができが良いので、まずそちらからお読みいただけますと幸いです。

Hey researchers (especially non-native speakers of English), do you use generative AI to write papers? If so, how do you (or how should we) acknowledge it? I haven't, but am thinking how to write next one.

Better late than never #alphafold3 (Chlamydomonas reinhardtii cytoplasmic dynein 1b heavy chain from Phytozome)

Bacterial rheotaxis is ENHANCED in non-Newtonian fluids! This work is in collaboration with Arnold Mathijssen and Albane Thery. Read more about our experiments and simulations in our new PNAS manuscript at pnas.org/doi/10.1073/... @sorheology.bsky.social @pnas.org

I made a (quite incomplete) starter pack of Japanese or in-Japan researchers. Self-identify, and I will add you! 日本人/日本にいる研究者のスターターパックを作りました。リプしていただければ追加します！ go.bsky.app/7kMz86x

I thought I had a picture of Ginkgo in the Koishikawa Botanical Garden of the University of Tokyo, but actually it was "精子発見のソテツ" (cycad from which sperm were discovered). April 2024

Tim Stearns@stearnslab.bsky.social · 2y ago

Ginkgo leaves are blanketing NYC in yellow. Little known #cilia fact: the sperm of Ginkgo trees are large cells with ~1000 cilia arranged in a spiral that have to swim a short distance to fertilize the egg. 1/2