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CinderBio

@cinderbio.bsky.social

Extreme enzymes for #proteomics and specialty applications. > One-step/5-minute digests w/ orthogonal coverage to trypsin > 1-hr 100% de novo sequencing > Discover novel biomarkers Link to Cell Systems and other peer-reviewed papers at CinderBio.com.

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ASMS@asms.org · 3mo ago

Congratulations to Albert J.R. Heck, recipient of the 2026 #ASMS John B. Fenn Award for Distinguished Contribution! A leader in the field, his work continues to shape the future of #massspectrometry. We’re excited to celebrate his achievements at #ASMS2026 in San Diego.

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CinderBio@cinderbio.bsky.social · last yr.

Learn about de novo antibody sequencing by mass spec using only one run at poster TP-005 today! #asms2025 #proteomics @hecklab.bsky.social @tshamorkina.bsky.social @tkadava.bsky.social @joostsnijder.bsky.social

Joost Snijder@joostsnijder.bsky.social · last yr.

These new archaeal proteases are great for de novo antibody sequencing by mass spec. Great work by @tshamorkina.bsky.social , Laura and @tkadava.bsky.social together with @hecklab.bsky.social. www.biorxiv.org/content/10.1...

Tatiana Shamorkina@tshamorkina.bsky.social · last yr.

We are excited to have it out: a combination of archaeal proteases and hybrid fragmentation for antibody de novo sequencing. Poster TP-005 #asms2025 #teammassspec It was a true team effort @lperezpaneda.bsky.social @tkadava.bsky.social @joostsnijder.bsky.social and @hecklab.bsky.social

PastelBio@pastelbio.bsky.social · last yr.

Deep Coverage and Extended Sequence Reads Obtained with a Single Archaeal Protease Expedite de novo Protein Sequencing by Mass Spectrometry www.biorxiv.org/cont... --- #proteomics #prot-preprint

Gary Siuzdak@siuzdak.bsky.social · last yr.

Krakatoa doesn't have to just mean violent destruction. Close to real-time proteeomics was achieved via a combined effort between @CedarsSinai and Cinder with their unique hypothermophile enzyme "Krakatoa" pubs.acs.org/doi/10.1021/...

Toward Real-Time Proteomics: Blood to Biomarker Quantitation in under One Hour

Multistep multihour tryptic proteolysis has limited the utility of bottom-up proteomics for cases that require immediate quantitative information. The power of proteomics to quantify biomarkers of hea...

pubs.acs.org

Simion Kreimer@simionkreimer.bsky.social · last yr.

Awesome paper, where we present and evaluate the methodology that will someday enable real-time monitoring of circulating biomarkers. pubs.acs.org/doi/full/10....

Toward Real-Time Proteomics: Blood to Biomarker Quantitation in under One Hour

Multistep multihour tryptic proteolysis has limited the utility of bottom-up proteomics for cases that require immediate quantitative information. The power of proteomics to quantify biomarkers of health status cannot practically assist in clinical care if the dynamics of disease outpaces the turnaround of analysis. The recently available hyperthermoacidic archaeal (HTA) protease “Krakatoa” digests samples in a single 5 to 30 min step at pH 3 and >80 °C in conditions that disrupt most cells and tissues, denature proteins, and block disulfide reformation thereby dramatically expediting and simplifying sample preparation. The combination of quick single-step proteolysis with high-throughput dual-trapping single analytical column (DTSC) liquid chromatography–mass spectrometry (LC–MS) returns actionable data in less than 1 h from collection of unprocessed biofluid. The systematic evaluation of this methodology finds that over 160 proteins are quantified in less than 1 h from 1 μL of whole blood. Furthermore, labile Angiotensin I and II bioactive peptides along with a panel of protein species can be measured at 8 min intervals with a 20 min initial lag using targeted MS. With these methods, we analyzed serum and plasma from 53 individuals and quantified Angiotensin I and II and over 150 proteins including at least 46 that were not detected with trypsin. We discuss some of the implications of real-time proteomics including the immediate potential to advance several clinical and research applications.

pubs.acs.org

CinderBio@cinderbio.bsky.social · 3y ago

We want to give a public "Thank You!” to early adopters of CinderBio's initial HTA-Proteases. We are thrilled to contribute to experiments in clinical proteomics, epigenetics, immunotherapies, cancer drug discovery and more. We enjoy being this kind of busy. Thanks to all of you - Team CinderBio

CinderBio@cinderbio.bsky.social · 3y ago

Venny has moved! After reading this blog post by @proteomicsnews.bsky.social I did a little digging and found this page: bioinfogp.cnb.csic.es where it says the site is down for maintenance. However, Venny is temporarily available here csbg.cnb.csic.es/BioinfoGP/ve... Happy Venning! #proteomics

VENNY VENN IS DOWN!

Okay.  I'm not going to panic, but VENNY doesn't work.  If you aren't familiar, the VENNY website by Juan Carlos Oliveros (2007) was the fir...

proteomicsnews.blogspot.com