UK ‘falling short’ in fight against rise of superbugs resistant to antibiotics

Antimicrobial resistance contributing to estimated 35,000 deaths a year in UK, and government ‘a long way’ from containing the problem, says NAO

theguardian.com

Job Specification

my.corehr.com

a dog is standing on a table in front of a christmas tree and a fireplace

ALT: a dog is standing on a table in front of a christmas tree and a fireplace

media.tenor.com

Improved detection of differentially abundant proteins through FDR-control of peptide-identity-propagation

Quantitative analysis of proteomics data frequently employs peptide-identity-propagation (PIP) — also known as match-between-runs (MBR) — to increase the number of peptides quantified in a given LC-MS/MS experiment. PIP can routinely account for up to 40% of all quantitative results, with that proportion rising as high as 75% in single-cell proteomics. Therefore, a significant concern for any PIP method is the possibility of false discoveries: errors that result in peptides being quantified incorrectly. Although several tools for label-free quantification (LFQ) claim to control the false discovery rate (FDR) of PIP, these claims cannot be validated as there is currently no accepted method to assess the accuracy of the stated FDR. We present a method for FDR control of PIP, called “PIP-ECHO” (PIP Error Control via Hybrid cOmpetition) and devise a rigorous protocol for evaluating FDR control of any PIP method. Using three different datasets, we evaluate PIP-ECHO alongside the PIP procedures implemented by FlashLFQ, IonQuant, and MaxQuant. These analyses show that PIP-ECHO can accurately control the FDR of PIP at 1% across multiple datasets. Only PIP-ECHO was able to control the FDR in data with injected sample size equivalent to a single-cell dataset. The three other methods fail to control the FDR at 1%, yielding false discovery proportions ranging from 2–6%. We demonstrate the practical implications of this work by performing differential expression analyses on spike-in datasets, where different known amounts of yeast or E. coli peptides are added to a constant background of HeLa cell lysate peptides. In this setting, PIP-ECHO increases both the accuracy and sensitivity of differential expression analysis: our implementation of PIP-ECHO within FlashLFQ enables the detection of 53% more differentially abundant proteins than MaxQuant and 146% more than IonQuant in the spike-in dataset. ### Competing Interest Statement The authors have declared no competing interest.

biorxiv.org

AMI Webinar: connecting, inspiring and advocating for LGBTQIA+ researchers in microbiology

appliedmicrobiology.org

Pandemic Preparedness - Purpose & Data Driven Testing

YouTube video by The Pandemic Institute

youtu.be