1/4 Excited to share our new preprint on phage genome editing: Targeted DNA nicking enables efficient, single-step and counterselection-free editing of bacteriophage genomes Preprint: lnkd.in/dv4g5kW6

🚀 Excited to share that I’ve been awarded an EMBO Installation Grant to establish my research group at the EMBL Partnership Institute, Vilnius University Life Sciences Center! The grant provides €50,000/year for 5 years to build an internationally competitive lab and advance genome editing research.

Our paper is out! Hiding in plain sight among Cas12a nucleases, Cas12a3 cleaves not its RNA target but the 3′ ends of tRNA. Huge thanks to all who made this possible, especially the Beisel lab, Biao & Dirk for the structure, & @sebastianglatt.bsky.social for all things tRNA. doi.org/10.1038/s415...

RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity - Nature

Cas12a3 nucleases constitute a distinct clade of type V CRISPR–Cas bacterial immune systems that preferentially cleave the 3′ tails of tRNAs after recognition of target RNA to induce growth arres...

nature.com

Openness means more than access. We’re pleased to announce that we now publish Replication Studies, helping make science more reliable, transparent, and trustworthy. buff.ly/Gebypvq

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - @helmholtz-hiri.bsky.social go.nature.com/4n7zDpG

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology

Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

go.nature.com

🚀 New publication alert! 🚀 We just published in @natbiotech.nature.com: 👉 Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes doi.org/10.1038/s415...

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes - Nature Biotechnology

Append editing of ADP-ribosyl to thymine is used for precise modifications in bacteria and eukaryotes.

doi.org

🚀 Join the Patinios Lab at the VU LSC - EMBL Partnership Institute for Genome Editing Technologies! We are looking for a Postdoctoral Researcher to join our growing team! 🔎

Good news on this 35th anniversary of the #Restoration of #Independence in #Lithuania! A new ERC project from a Lithuanian researcher can now go ahead, as they were able to secure alternative EU funding for her ERC Consolidator Grant. How did this happen? Find out in the thread 🧵👇

Excited to see our SIBR work out in Trends in Biotechnology :) doi.org/10.1016/j.ti... This is the first study I have coordinated from start to finish, and it brings me immense satisfaction to see it published. For a brilliant description of the tech check this: www.linkedin.com/feed/update/...

SIBR2.0 is now out in a preprint!! doi.org/10.1101/2024...

Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system

Cupriavidus necator H16 is a promising microbial platform strain for CO2 valorisation. While C. necator is amenable to genome editing, existing tools are often inefficient or rely on lengthy protocols...

doi.org

How about a new genome editing tool?? #Append Editing, #ADPr-TAE, #Homologous recombination, #Base mutagenesis. doi.org/10.1101/2024... Thread: 🧵

Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes

Base editors create precise genomic edits by directing nucleobase deamination or removal without inducing double-stranded DNA breaks. However, a vast chemical space of other DNA modifications remains ...

doi.org