A multivalent RNA affinity tag enables selective purification of tagged RNAs and bound proteins doi.org/10.1093/nar/...

A multivalent RNA affinity tag enables selective purification of tagged RNAs and bound proteins

Abstract. Efficient purification of specific RNAs from lysates remains a major challenge. Existing methods, such as MS2 tagging or oligonucleotide hybridiz

doi.org

Shocking.

Programming tip: never type out a 10-character command when you could instead press the up arrow for 5 minutes until you find that command in your history

A couple paid more than $800,000 for a gene-editing therapy for their daughter. She died, and it wasn’t made public. An exclusive from us and @science.org.

Exclusive: Death of girl in Chinese gene-editing trial was never made public

Parents want accountability after study went disastrously wrong, Science and Retraction Watch investigation reveals

science.org

This "scalable" template for oral examination requires 20 minutes per student per exam, or 67 total hours for a class of 200 students. It would appear the authors and I have a different understanding of what "scalable" means. www.insidehighered.com/opinion/care...

A Practical, Scalable Template for Oral Exams (opinion)

Yes, it can be done, even in classes with dozens of students.

insidehighered.com

Submit your research on designed branched sheethelix proteins now

Luca Pinello@lucapinello.bsky.social · 3w ago

1/ I'm excited to share that we're launching NECB 2026, the inaugural New England Computational Biology Symposium. Oct 1-2 at Microsoft Research New England, Cambridge. Two days of keynotes,talks, and posters to bring our community together across institutions.Space is limited. newenglandcompbio.org

Ironically, as @mart1oeil.eu showed, there are telltale signs that the piece was edited with ChatGPT: many links include "?utm_source=chatgpt.com" If even Science editorial team cannot afford good human editing, that's worrying

Jed Brown@jedbrown.org · 3w ago

Editor in Chief of Science: AI in scientific publishing: Slower, worse, and more expensive.

1/10 🚨Preprint! One RING to rule them all (phages)👑💍⚔️ Bacteria build giant DNA-scaffolded RNase rings (larger than the ribosome!) to shut down viral infection🧬🧵👇 biorxiv.org/content/10.6...

Couldn't care less about football but love meself a random chart

Simon Kuestenmacher@simongerman600.bsky.social · 3w ago

For the first time, the World Cup final four teams includes only the top 4 teams in FIFA world rankings (1+2+3+4). The combined ranks of the last four teams couldn't be lower (10). In the past, the four last teams have always added up to over 25. HT @chartycharts

🚨 We're hiring! My new lab at EMBL Hamburg is looking for a Research Technician. I'm looking for someone excited by phages, metabolism, enzymes, structural biology and bacterial immunity—if you know the right person, please share this post!🧵⬇️ Apply here: embl.wd103.myworkdayjobs.com/en-US/EMBL/j...

Research Technician (Osterman Group – Metabolism-driven immunity)

EMBL Hamburg / CSSB The European Molecular Biology Laboratory (EMBL) is Europe’s flagship life sciences organization, with more than 110 independent research groups across Heidelberg, Hamburg, Grenobl...

embl.wd103.myworkdayjobs.com

Domain swap is a staple in protein engineering. But instead of trial & error, can we predict an ideal crossover w/ comp modeling? Happy to share a wonderful collaboration w/ Jürgen @lassak-lab.bsky.social at my alma mater LMU Munich to convert a protein ligase to an aaRS for β amino acids t.ly/lNpVw

The @qedscience.bsky.social "impact" score generated a lot of discussion on ranking preprints, including ideas on multi-dimensional rankings that are user specific. Besides describing the ideas, we can now prototype them (with Claude in this case). Here is Salient salient-sgeu6fs5ua-uc.a.run.app

Not Claude advertising their new model to me using a left-handed helix

Please note the image is from AI and is not scientifically accurate. We intended to add visual appeal to the post, and apologize for posting this fanciful but unfortunately misleading depiction. In future posts, we will ensure scientific images are based on actual data.

Roche throws the axe 🪓 at illumina with Axelios open.substack.com/pub/albertvi...

Roche throws the axe at Illumina with Axelios

What changes, and what doesn't, now that SBX has a commercial SKU

open.substack.com

Can't believe it Sent a cryo dewar with crystals via #FedEx to @esrf.fr and they lost it. Since 2 weeks gone without a trace and of course they're doing nothing to try find it. Equipment worth €8000, dozens of priceless xtals from our projects gone 4ever 😭 Compensation? €600 from FedEx (by weight) 😡

Beautiful videos nicely explaining microtubules! Congratulations to Renaud Chabrier and Casten Janke 👍 www.youtube.com/watch?v=dVbv...

Microtubules | Scientific Animated Short Film 2026

YouTube video by Drawing Intelligence

youtube.com

Since the 1960s, the genetic code has been used to predict protein sequences from DNA and mRNA sequences. Our @nature.com article demonstrates that these predictions miss thousands of protein sequences present in human tissues. www.nature.com/articles/s41... 1/

Nikolai Slavov@slavov-n.bsky.social · 2y ago

We report many proteins not predicted by the genetic code. They are stable & abundant O( 10³ ) copies / cell. Generative mechanisms include codon-anticodon mismatches & RNA modifications. Their abundance depends on codon frequency & protein stability. www.biorxiv.org/content/10.1...

Here is an example of what I expect we will start seeing more of, a single author bioinformatics paper that looks like it was made with AI assistance. The figures look very much straight out of Claude Code. It does have a "Use of AI tools" disclaimer section journals.plos.org/ploscompbiol...

A zero-parameter first-principles gate framework for full-length TP53 missense variant interpretation

Author summary Most tools that predict whether a TP53 mutation is disease-causing rely on machine learning trained on large datasets. These models can be accurate but typically cannot explain why a pa...

journals.plos.org

wtaf biology, as if you hadn't had enough enzyme mechanisms for adding oxygen already

Zeynep Baharoglu@zeynepbaharoglu.bsky.social · 2mo ago

Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO www.nature.com/articles/s41... 🦠 cryo-electron microscopy structure of Bacillus subtilis TrhO–tRNAAla complex

Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO www.nature.com/articles/s41... 🦠 cryo-electron microscopy structure of Bacillus subtilis TrhO–tRNAAla complex

Unconventional monooxygenation by the O2-dependent tRNA wobble uridine hydroxylase TrhO - Nature Chemical Biology

Shin et al. report the cryo-electron microscopy structure and catalytic mechanism of TrhO, a cofactor-independent enzyme that hydroxylates tRNA wobble uridine using molecular oxygen through a…

nature.com

I wrote about AI in academia. "PhD-level thinking", LLM bias, grunt work, alignment, AGI, data center water use, AI politics -- something for everyone.

Thoughts on AI in academia

PhD-level thinking, LLM bias, alignment, AGI, data centers, and AI politics

open.substack.com

Grep this: Microsoft grafts (most) Linux commands onto Windows

Grep this: Microsoft grafts (most) Linux commands onto Windows

Coreutils serves over 75 Unix commands in Windows and PowerShell command lines

theregister.com

well if you liked this you might also be interested in the graph I made for the same course on the abundance of elements. Green are the CHNOPS element, which constitute 98% of living matter on earth. Fun to speculate about the ones way off the axis

Max Fürst@maxfus.bsky.social · 2mo ago

Made this graph for my undergrad lecture

Why does tryptophan have only one codon, UGG?

Paying peer reviewers works. Expanded Fast & Fair experiment @biologyopen.bsky.social: • 5.5 vs 37.7 working days to decision with reviews • ~3 vs ~9 reviewer invitations per manuscript • no reduction in editor-assessed review quality • similar acceptance rates www.biorxiv.org/content/10.6...

Earlier this week I posted an example of a fake western blot provided by ThermoFisher to demonstrate the validity of a p53 antibody. I considered it an amusing curiosity. In fact Thermo Fisher Scientific has systematically manipulated antibody validation data!

Took a while, but happy to share the published version of our article on ADDing, our highly efficient new enzymatic protein labelling platform that made it on the cover of ACS bioconjugate chemistry 😁 pubs.acs.org/toc/bcches/c...

Bioconjugate Chemistry

The molecular structure of flavin transferase ApbE in complex with an ADD tag-bearing protein substrate, GFP: The transferase is depicted in the process of installing the click handle onto the target threonine, facilitated by the use of the ADD substrate that is generated enzymatically with ADP-ribosyl cyclase from NAD+. View the article.

pubs.acs.org

Max Fürst@maxfus.bsky.social · last yr.

🚨 preprint 2️⃣ this month: our (purely experimental🧪) venture into #ChemBio We prouldy present: ADD-tagging of proteins (or "ADDing") —a super convenient enzymatic technique to install click chemistry handles on proteins. Led by superstar @wahyuwidodo.bsky.social www.biorxiv.org/content/10.1... A 🧵👇🏽

Screen 1M random protein sequences to discover that biology-like folds are accessible from random sequences with surprising frequency www.biorxiv.org/content/10.6...

PDBe-SIFTS is now open source 🎉 Developed in collaboration with PDBe (@pdbeurope.bsky.social) and UniProt, it enables fast, accurate residue-level mapping between protein sequences and structures, achieving >93% agreement with curated mappings. Get started: github.com/PDBeurope/SI...