I am delighted to share our latest paper on mycomembrane protein assembly which came out last week in @pnas.org This was a fantastic collaboration between my former colleague, Elizabeth (#Betsy) Hart, and my first PhD student, Dieuwertje (#Didi) de Bruin. www.pnas.org/doi/10.1073/... (1/n)

Latest from the lab! Kevin England @kevinengland.bsky.social leads a study on how production of various alarmones (p,pp,pppGpp!) depletes GTP, remodels the transcriptome, and inhibits translation. Wonderful collaboration with Jade Wang's lab at UW Madison. academic.oup.com/nar/article/...

The alarmone pGpp inhibits translation through GTP depletion

Abstract. Bacteria produce the alarmone nucleotides ppGpp and pppGpp during stress to affect replication, transcription, and metabolism. ppGpp and pppGpp a

academic.oup.com

Our paper (and my first first-author) is out! The alarmone pGpp inhibits translation through GTP depletion academic.oup.com/nar/article/... 🦠🚨🤠 @narjournal.bsky.social

Structural basis for nutrient-activated channel opening in bacterial spore germination receptors https://www.biorxiv.org/content/10.64898/2026.08.27.747515v1

This is now published! For those interested in microbial mechanotransduction: www.cell.com/current-biol...

Microbial GAIN domains undergo autoproteolysis and enable release of diverse cell-surface-associated proteins

Brogan and Rudner report the identification of a microbial autoproteolytic domain that is structurally homologous to the GAIN domain of eukaryotic adhesion GPCRs. Rather than participating in transmem...

cell.com

anna brogan@apbrogan.bsky.social · 5mo ago

My latest work! We found that the autoproteolytic GAIN domain which mediates force responsive signaling in adhesion GPCRs is not unique to eukaryotes. The microbial counterparts anchor diverse adhesion, enzymatic, and toxin domains to the cell surface, enabling release by likely mechanical stimuli.

Another chapter of my thesis is out! We asked whether we could pair proteome-wide AlphaFold screening with Tn-seq to identify biologically relevant protein-protein interactions. We identify ClcR (formerly YerH) as a component of the Rod complex in Gram-positive bacteria. www.pnas.org/doi/10.1073/...

A broadly conserved gram-positive lipoprotein regulates cell elongation | PNAS

The cell wall peptidoglycan (PG) protects virtually all bacteria from osmotic lysis and specifies cell shape. Synthesis of this exoskeleton is carr...

pnas.org

I’m so honored to be included in this Hypothesis Fund cohort and grateful for this investment in our research! 🥲

Hypothesis Fund@hypothesisfund.bsky.social · 6mo ago

📣 Bold science alert! We're thrilled to announce our latest awardees who are pushing the boundaries of research in aging, regeneration, and bacterial resilience. bit.ly/HFAwardees Hear from the #HFScouts who championed these talented scientists👇 in our 🧵: 1/

rince & JB Editor's Choice: Lin, Prince & Feaga identified sporulation genes via analyzing almost 1500 genomes. They show that that an unusual membrane-localized variant of Spo0B is found throughout Paenibacillaceae. journals.asm.org/doi/10.1128/... @asm.org #JBacteriology

The sporulation phosphorelay protein Spo0B is membrane localized in Paenibacillus! The transmembrane domain appears to increase interaction with relay partners. Congrats to former lab undergrad @isabellalin.bsky.social and her grad mentor @cassidyprints.bsky.social journals.asm.org/doi/10.1128/...

Here we show that >90% of Paenibacillus encode a unique Spo0B with a transmembrane domain! TM domain helps it interact with its phosphorelay partners. Congrats to @isabellalin.bsky.social who left for grad school this week and her mentor @cassidyprints.bsky.social! www.biorxiv.org/content/10.1...

Conservation of sporulation genes and a transmembrane-containing Spo0B variant in Paenibacillus

Sporulation is a strategy employed by many bacteria to survive harsh environmental conditions. The genus Paenibacillus includes spore-forming species notorious for spoiling pasteurized dairy products ...

biorxiv.org

Bella Lin @isabellalin.bsky.social will be attending Harvard BBS for her PhD!! We’re so proud of you Bella! 🍾🎉