We organized a workshop about our new SMT software FreeTrace at QLIFE in ENS Paris. www.edu.bio.ens.psl.eu/spip.php?art... It was our second workshop following Mifobio 2025! We warmly thank all the participants 😊
@asaki-kobayashi.bsky.social
Happy to share the last part of my PhD work academic.oup.com/nar/article/...
U2AF2 controls alternative splicing in speckle-proximal regions in an RS domain-dependent manner
Abstract. Splicing factor U2AF2 is known to play a pivotal role for 3′ splice site recognition at an early step of spliceosome assembly. Here, using proxim
academic.oup.com
Its been a pleasure to be a part of this work. Our group, and in particular Junwoo Park, describes a powerful tool to study single-molecule tracking. FreeTrace reconstruct trajectories under fractional Brownian motion, which enabled us to study anomalous diffusion 🔬 www.biorxiv.org/content/10.6...
Novel estimation of memory in molecular dynamics with extended and comprehensive single-molecule tracking software: FreeTrace
Single-molecule tracking (SMT) in live cells reveals how biomolecules explore crowded intracellular environments, yet most tracking software assumes Brownian motion, an approximation that fails when a...
biorxiv.org
SPT of DNA repair proteins after cell irradiation by Ashli Kobayashi @asaki-kobayashi.bsky.social and Romane Riou. Setup by Abbelight @abbelight.bsky.social and irradiator by Errol Laser @errol-laser.bsky.social. Look at that recruitment! #Mifobio2025 @gdrimabio.bsky.social
We are looking forward to the workshop "Biomolecular Condensates and Polymer Phase Transitions" which we are organizing together with the IPF Dresden (dpd.ipfdd.de). It will be held from May 25th - 28th, 2025 in Meißen, near Dresden. The registration deadline is May 5th, 2025.
The nucleoid of rapidly growing Escherichia coli localizes close to the inner membrane and is organized by transcription, translation, and cell geometry | Nature Communications https://www.nature.com/articles/s41467-025-58723-4
The nucleoid of rapidly growing Escherichia coli localizes close to the inner membrane and is organized by transcription, translation, and cell geometry - Nature Communications
The mechanisms underlying bacterial chromosome configuration are not fully understood. Here, Spahn et al. show that the Escherichia coli nucleoid adopts a condensed, membrane-proximal configuration during rapid growth, with transcription and translation acting as main drivers of nucleoid organization.
nature.com
I am thrilled to share this new preprint from our lab! We find (>800!) 30S binding sites in hundreds of 5'UTRs, but also new dynamic steps of translation initiation. www.biorxiv.org/content/10.1... Congrats to all co-authors at the RNA control of gene expression lab, IBPC CNRS (Thread, 1/6)
30S-seq redefines the bacterial Ribosome Binding Site
The translation initiation step is rate limiting for the efficiency of gene expression in all organisms. However, the mechanism of ribosome recruitment to mRNA start sites strikingly differs between e...
biorxiv.org
Happy to share our genetic toolkit designed to make your gene editing (life) easier, faster and very efficient! Also suitable to build saturated chromosomal variant libraries using oligo-recombineering with extra short homology arms! www.biorxiv.org/content/10.1...
bioRxiv Microbiology@biorxiv-microbiol.bsky.social · last yr.EASY-CRISPR: a toolbox for high-throughput single-step custom genetic editing in bacteria https://www.biorxiv.org/content/10.1101/2025.03.20.644322v1
I am glad to share our detailed super resolution imaging protocol for single particle tracking in human cell. doi.org/10.1101/2025...
A guide for Single Particle Tracking: from sample preparation and image acquisition to the analysis of individual trajectories
Live-cell Single-Particle Tracking (SPT) is a powerful super resolution microscopy approach. It allows us to observe live recordings of individual molecules in living cells at high temporal and spatia...
doi.org